ISOLASI DNA METAGENOMIK DARI  TANAH HUTAN MANGROVE PANTAI SUWUNG BALI

I Nengah Wirajana; Darma Asih Yuliana; Ketut Ratnayani

doi:10.24843/JCHEM.2013.v07.i01.p03

ISOLASI DNA METAGENOMIK DARI TANAH HUTAN MANGROVE PANTAI SUWUNG BALI

I Nengah Wirajana
Darma Asih Yuliana
Ketut Ratnayani

DOI: https://doi.org/10.24843/JCHEM.2013.v07.i01.p03

Abstract

Metagenomic DNA isolation from mangrove forest soils of Suwung Beach-Bali was conducted to exploit the biocatalytic potential of microbial communities for the discovery of novel cellulase. The whole DNA isolation was conducted by modification of preparation step by Marco (2010) and DNA extraction step by Amorim et al (2008). The results of metagenomic DNA isolation were analyzed by spectrophotometry and agarose gel electrophoresis. The results showed that whole DNA was able be isolated successfully, but protein and humic acid were found as contaminant.

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Author Biographies

I Nengah Wirajana

Jurusan Kimia FMIPA Universitas Udayana, Bukit jimbaran

Darma Asih Yuliana

Jurusan Kimia FMIPA Universitas Udayana, Bukit jimbaran

Ketut Ratnayani

Jurusan Kimia FMIPA Universitas Udayana, Bukit jimbaran

How to Cite

WIRAJANA, I Nengah; ASIH YULIANA, Darma; RATNAYANI, Ketut. ISOLASI DNA METAGENOMIK DARI TANAH HUTAN MANGROVE PANTAI SUWUNG BALI. Jurnal Kimia (Journal of Chemistry), [S.l.], july 2013. ISSN 2599-2740. Available at: <https://ojs.unud.ac.id/index.php/jchem/article/view/5980>. Date accessed: 21 nov. 2024. doi: https://doi.org/10.24843/JCHEM.2013.v07.i01.p03.

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