AKTIVITAS ANTIBAKTERI SENYAWA GOLONGAN TRITERPENOID DARI BIJI PEPAYA (Carica papaya L.)

Abstract

Isolation, identification, and antibacterial activity examination have been conducted for triterpenoids
compounds of carica papaya L. Maseration of 500 g pawpaw seed powder in n-hexane gave 21.66 g extract which
reacts with Liebermann-Buched reagent resulting in colour change (yellow to purple). Column chromatography
separation of the extract resulted in 0.05 g yellow isolate (F3) which contains aldehyde triterpenoids. Infrared spectra
indicated aliphatic –C-H stretching, -CH2 bending, -CH3 bending, and C=O. Ultra violet – visible spectra showed
absorption at 228.5 nm and proad peak at 287.7 nm. Antibacterial axamination showed that 1000 ppm at the isolate
inhibits the growth of Escherichia coli and Staphylococcus auerus.