• I Putu Priyasana Program Studi Farmasi Universitas Udayana
  • Gusti Ayu Dinda Mayagita PRogram Studi Farmasi Universitas Udayana
  • Vallina Rahmadinha Program Studi Farmasi Universitas Udayana
  • Kristina Megi Limba Program Studi Farmasi Universitas Udayana
  • Pande Made Nova Armitasari PRogram Studi Farmasi Universitas Udayana


Opiates are a group of narcotics that are often abused. Forensic experts play crucial roles in handling this. The screening test results that the sample containing opiate compounds. Confirmation testing is carrie out by using TE and TAE mobile phase systems. The error window values ??obtained in the TE and TAE systems are 10 and 8. Data collection of compounds in the range of hRfc 16.09 to 36.09 in the TE system obtained two compounds, Morphine and Codeine). And in the range of hRfc 25.1-9.1 also found Morphine and Codein compounds. The results of the correlation spectrum of the sample and spectrum in the library showed a correlation value of 0.99079 (TE system) and 0.99477 (TAE system) to the codeine compound. Determination testing is carried out using the TE mobile phase. This test results obtained that the concentration of codeine compounds is 20.88 ng / µL. These results indicate that the sample contains codeine in the lethal dose range.


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[1] Ahadi, A., Partoazar, A., Abedi-Khorasgani, M. H., Shetab-Boushehri, S. V. Comparison of Liquid-Liquid Extraction-Thin Layer Chromatography with Solid-Phase Extraction-High Performance Thin Layer Chromatography in Detection of Urinary Morphine. Journal of Biomedical Research 2011; 25(5):362-367.
[2] Badan Narkotika Nasional. Pedoman Pemeriksaan Laboratoirum Narkotika, Psikotropika dan Obat Berbahaya. Jakarta: Badan Narkotika Nasional; 2008.
[3] Berg H, Lunaden E, Christophersen AS, and Strand DH. Determination of Opiates and Cocaine in Urine by High pH Mobile Phase Reversed Phase UPLC-MS/MS. Journal of Chromatography B. 2009; 877(2009): 421-432.
[4] Gandjar, I. B. and Rohman, A. Kimia Farmasi Analisis. Yogyakarta:Pustaka Pelajar; 2007.
[5] Liu RH and Gadzala DE. Handbook of Drug Analysis. Washington DC: American Chemical Society; 1997.
[6] Moffat AC, Osselton MD, and Widdop B. Clarke’s Analysis of Drugs and Poisons. Pharmaceutical Press; 2005
[7] Presiden RI. Undang-Undang Republik Indonesia Nomor 35 Tahun 2009 tentang Narkotika. Jakarta: Presiden Republik Indonesia; 2009.
[8] Rodrigues, A., M. Yegles, N. V. Elsue, and S. Schneider. Determination of Cannabinoid in Hair of CBD Rich Extracts Consumers using Gas Chromatography With Tandem Mass Spectrometry (GC/MS-MS). Forensic Science International; 2018.
[9] Sari PMNA and Leliqia NPE. Uji Skrining dan Determinasi Kodein dengan TLC-Spektrofotodensitometri. Indonesian Journal of Legal and Forensic Sciences. 2013; 3(1): 26-31.
[10]Skoog DA, Holler FJ, Crouch SR. Principles of instrumental analysis. 7th ed. USA: Cengage Learning; 2018
[11] Wirasuta, I.M.A.G. Analisis Toksikologi Forensik dan Interpretasi Temuan Analisis. Indonesian Journal of Legal and Forensic Science 2008; 1(1):47-55.
How to Cite
PRIYASANA, I Putu et al. SCREENING AND DETERMINATION OF CODEIN IN URINE SAMPLE. Indonesian Journal of Legal and Forensic Sciences (IJLFS), [S.l.], v. 10, n. 1, p. 30 - 37, june 2020. ISSN 2657-0815. Available at: <>. Date accessed: 21 jan. 2021. doi: