POTENSI ANTIIMPLAMASI SECARA IN VIVO EKSTRAK ETANOL BATANG ANTAWALI (Tinospora sinensis) PADA TIKUS WISTAR YANG DIINDUKSI  KARAGENAN

Ida Ayu Putu Sri Adnyasari; Ni Made Puspawati; I Made Sukadana

Ida Ayu Putu Sri Adnyasari Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Bali - Indonesia
Ni Made Puspawati Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Bali-Indonesia
I Made Sukadana Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana, Bali - Indonesia

Abstract

ABSTRAK: Penelitian ini bertujuan untuk menguji aktivitas antiinfamasi dari ekstrak etanol batang antawali (Tinospora sinensis) dan mengidentifikasi kandungan kimianya. Uji aktivitas antiinflamasi dilakukan secara in vivo dengan metode induksi karagenan, sedangkan identifikasi kandungan kimia secara kualiatif dengan uji fitokimia. Penelitian ini menggunakan 25 ekor tikus putih jantan galur Wistar yang dibagi menjadi 5 kelompok yaitu kontrol negatif (P₀), kontrol positif (P₁), dan kelompok ekstrak uji (P₂, P₃, dan P₄) dengan dosis 125 mg/kg BB, 250 mg/kg BB, dan 500 mg/kg BB. Hasil uji aktivitas antiinflamasi menunjukkan bahwa semakin rendah dosis yang diberikan, maka akan menghasilkan persentase hambatan inflamasi yang semakin besar. Pemberian dosis 125 mg/kg BB mampu menghasilkan persentase hambatan tertinggi, diikuti dengan dosis 250 mg/kg BB, serta 500 mg/kg BB yaitu masing-masing sebesar 64,71%; 52,94%; dan 29,41% pada menit ke-360. Ekstrak etanol batang antawali memiliki nilai ED₅₀sebesar 51,521 mg/kg BB. Secara statistik dengan uji One Way ANOVA menunjukkan nilai p<0,05. Hasil uji fitokimia menunjukan bahwa ekstrak etanol batang Tinospora sinensis mengandung senyawa alkaloid, fenolik, dan steroid.

ABSTRACT: This study aimed to evaluate the antiinflammatory activity of ethanolic extract of antawali stem (Tinospora sinensis) and to identify its phytochemical constituents. In vivo antiinflammatory activity test was performed on Wistar rat oedem induced by carrageenan, while identification of chemical constituents was done with phytochemical screening. In this study, 25 male Wistar strains were divided into 5 groups : negative control (P₀), positive control (P₁), test extract group (P₂, P₃, and P₄) with doses of 125 mg/kg BW, 250 mg/kg BW, and 500 mg/kg BW. Antiinflammatory activity test results showed that the lower the dose given, it will show a greater percentage of inflammatory inhibition. The dose of 125 mg/kg BW was able to produce the highest percentage of inhibition, followed by doses of 250 mg/kg BW, and 500 mg/kg BW at 64.71%; 52.94%; and 29.41% in the 360^th minute. The extract of ethanol stem antawali active as antiinflamasi with ED₅₀ that was equal to 51,521 mg/kg BB. Statistic One Way ANOVA test showed p value < 0,05. The result of phytochemical screening showed that stem ethanolic extract of Tinospora sinensis contains alkaloids, phenolic, and steroids.

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