MOLECULAR IDENTIFICATION OF BLASTOCYSTIS SP. IN LONG-TAILED MACAQUE AT ALAS PURWO PARK

Edward Yonas Kristijanto; Wiwik Misaco Yuniarti; Mufasirin Mufasirin; Poedji Hastutiek; Endang Suprihati; Boedi Setiawan; Puput Ade Wahyuningtyas; Doohan Mahendra

doi:10.24843/bulvet.2023.v15.i05.p05

Edward Yonas Kristijanto Master Student, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia
Wiwik Misaco Yuniarti Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia
Mufasirin Mufasirin Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia
Poedji Hastutiek Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia
Endang Suprihati Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia
Boedi Setiawan Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia
Puput Ade Wahyuningtyas Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia
Doohan Mahendra Balai Karantina Pertanian Kelas II Palu, Indonesia

DOI: https://doi.org/10.24843/bulvet.2023.v15.i05.p05

Abstract

Zoonotic diseases can be transmitted through close interactions between long-tailed monkeys. Blastocystis sp. is one of the parasites that can attack mammals and is most commonly found in the intestinal tract. This study aims to analyze the presence of infection and the phylogenetic tree of Blastocystis sp. in long-tailed monkeys in Alas Purwo National Park, Banyuwangi, East Java. Identification of Blastocystis sp. in this study using morphological and molecular methods. A total of 100 stools were examined microscopically using the floating method, showing that 61 samples were positive, followed by a PCR test with a target of 600bp. PCR results obtained three positive samples followed by squencing. Sequences processed in BLAST isolate samples TNAP2 and TNAP9 having homology with Blastocystis sp. Subtype 3 was 98-99%, while the TNAP7 isolate had a lower homology level of 78-79% and the level of phylogenetic analysis of the TNAP2 and TNAP9 isolates was related to Blastocystis sp. from the Philippines (KY610153.1) and Egypt (OP942294.1) and the TNAP7 isolate is related to Blastocystis sp. from Thailand (MH197670.1, MH197668.1). Isolate Blastocystis sp. from Alas Purwo National Park has high homology analysis results to Blastocystis type hominis from Rep. Czech and Chinese by 80-99%, it is possible to have a connection with the zoonotic problem.

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