CORRELATION OF CERVIC MUCOUS VISCOSITY WITH ESTROGEN CONCENTRATION DURING ESTRUS IN ACEH CATTLE
Abstract
The viscosity of cervical mucus is affected by the concentration of estrogen. This study aims to determine the relationship between the viscosity of cervical mucus with the concentration of estrogen in Aceh cattle in the success of mating. This study used four female Aceh cattle, aged 3-7 years and weighing 150-250 kg, had given birth, and had two regular estrus cycles. All aceh cattle were synchronized with PGF2? (dinoprost tromethamine inj., Lutalyse®, Zoetis US) by the two shoot method intramuscularly. Cervical mucus collection was performed by aspiration on the day of estrus. The cervical mucus viscosity measurement is done by looking at the spinnbarkeit value. Estrogen concentration was measured by enzyme linked immunosorbant assay (ELISA). The data obtained in this study were analyzed using the Pearson correlation test. The results showed that the average viscosity of cervical mucus in Aceh was 11.8±4.6 cm, and the average concentration of estrogen in Aceh cattle during estrus was 106.5±15.8 pg/ml. The correlation between cervical mucus viscosity and estrogen concentration was 0.983. The conclusion of this study is that the higher the concentration of estrogen, the viscosity of cervical mucus will decrease so that the spinnbarkeit value will increase. It is necessary to conduct further studies related to the electrochemical characteristics of cervical and vaginal mucus during estrus including the value of ferning typology, electrical resistance vaginal mucus (ERVM), pH, sodium, magnesium, calcium and other cations that affect estrus performance in Aceh cattle.
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References
Adinegara R. 2006. Penambahan etinil estradiol pada induksi ovulasi menggunakan klomifen sitrat. Pengaruh Terhadap Nilai Rheologi Lendir Serviks dan Ketebalan Endometrium. Tesis. Program Pasca Sarjana Universitas Diponegoro, Semarang.
Chaudhary R. 2019. Adrenalin. In: Vonk, J., Shackelford, T. (eds) Encyclopedia of Animal Cognition and Behavior. Springer, Cham. https://doi.org/10.1007/978-3-319-47829-6_1439-1.
Direktorat Jendral Peternakan dan Kesehatan Hewan Kementerian Pertanian. 2020. Statistik Peternakan dan Kesehatan Hewan. Kementrian Pertanian RI. Jakarta.
Hanumant D, Tiwari RP, Chaturvedani AK, Paikra D, Chandrakar C, Ratre P. 2019. Analysis of corporeal characteristics of cervico-vaginal mucus in cows. Pharma Innov. 8(3): 261-264.
Irmaylin SM, Hartono M, Santosa PE. 2014. Respon kecepatan timbilnya estrus dan lama estrus pada berbagai paritas sapi peranakan ongole (PO) setelah dua kali penyuntikan prostaglandin F2α (PGF2α). J. Ilmiah Peternakan Terpadu. 2(1): 41-49.
Kumar A, Mehrotra SS, Dangi SS, Singh G, Singh M, Mahla AS. 2012. Amylase activity in cervical mukus and serum during estrus in normal and repeat breeder cattle. Vet. World. 5(8): 486-488.
Lacroix G, Gouyer V, Gottrand F, and Desseyn JL. 2020. The cervicovaginal mucus barrier. Int. J. Mol. Sci. 21(21): 8266.
Laksmi DNDI, Trilaksana IGNB. 2020. The change in external genital and estrogen level of Bali cattle during estrus. J. Vet. Anim. Sci. 3(1): 40-50.
Mardiati SM. 2003. Kadar garam Na lendir servik serta kadar garam Na dan K lendir mulut pada berbagai struktur daun pakis (test ferning). Magister Ilmu Biomedik. Universitas Diponegoro, Semarang.
Nakano FY, Leão RDBF, Esteves SC. 2015. Insights into the role of cervical mucus and vaginal pH in unexplained infertility. Med. Express. 2(2): M150207.
Panjaitan B, Pambudi R, Amansyah R, Akmal M, Siregar TN. 2020. Kadar estrogen darah dan tingkat keasaman (pH) mukus serviks sapi aceh memengaruhi daya penetrasi spermatozoa. J. Vet. 21(3): 485-492.
Perumamthadathil CS, Johnson WH, Leblanc SJ, Foster RA, Chenier TS. 2014. Persistence of oxytocin receptors in the bovine uterus during the first 7 d after calving: an immunohistochemical study. Can. J. Vet. Res. 78(1): 72-77.
Purwaningsih W, Samsudewa D, Ondho YS. 2018. Profil lendir serviks rusa timor (Cervus timorensis) betina yang mendapat suplementasi mineral pada tiap fase berahi. J. Sain Peternakan Indonesia. 13(2): 202-213.
Putri. AN, Surhayati S, Santosa PE. 2014. Pengaruh paritas terhadap persentase estrus dan kebuntingan sapi peranakan Ongole yang disinkronisasi estrus dengan menggunakan prostaglandin F2 (PGF2). J. Pet. 2(2): 31-36.
Ramli M, Siregar TN, Thasmi CN, Wahyuni S, Sayuti A. 2016. Hubungan antara intensitas estrus dengan konsentrasi estradiol pada sapi aceh pada saat inseminasi. J. Medika Vet. 10(1): 27-30.
Silaban NL, Setiatin ET, Sutopo. 2012. Tipologi ferning sapi jawa brebes betina berdasarkan periode birahi. Anim. Agricult. J. 1(1): 777-788.
Singh LK, Pandey M, Baithalu RK, Fernandes A, Ali SA, Jaiswal L, Pannu S, Neeraj, Mohanty TK, Kumaresan A, Datta TK, Kumar S, Mohanty AK. 2022. Comparative Proteome Profiling of Saliva Between Estrus and Non-Estrus Stages by Employing Label-Free Quantitation (LFQ) and Tandem Mass Tag (TMT)- LC-MS/MS Analysis: An Approach for Estrus Biomarker Identification in Bubalus bubalis. Front. Genet. 13:867909.
Siregar TN, Armansyah T, Panjaitan B, Gholib G, Herrialfian H, Sutriana A, Abidin Z, Reynaldi MA, Razak F, Artaliani Y, and Yuswar Y. 2019. Changes in cervical mucus as an indicator of fertility in Aceh Cattle. Adv. Anim. Vet. Sci. 7(4): 306-314.
Suharto K. 2003. Penampilan potensi reproduksi sapi perah friesian holstein akibat pemberian kualitas ransum berbeda dan infusi larutan iodium povidon 1% intra uteri. Program Studi Magister Ilmu Ternak Program Pasca Sarjana Fakultas Peternakan Universitas Diponegoro, Semarang.
Susilawati T. 2011. Tingkat keberhasilan inseminasi buatan dengan kualitas dan deposisi semen yang berbeda pada sapi Peranakan Ongole. TERNAK TROPIKA J. Trop. Anim. Prod. 12(2): 15-24.
Thasmi CN, Siregar TN, Wahyuni S, Aliza D, Hamdan H, Panjaitan B, Asmilia N, Husnurrijal H. 2017. Estrus performance and steroid level of repeat breeding Aceh cattle synchronized with PGF2 alfa. Veterinaria. 66(1): 36-41.
Thornton DJ, Rousseau K, McGuckin MA. 2008. Structure and function of the polymeric mucins in airways mucus. Annu. Rev. Physiol. 70: 459-486.
Trisunuwati P. 2016. The role of leaf water clover (Marsilia crenata) squeeze towards estrogen blood level and uterine histology in rats (Rattus norvegicus). J. Ternak Tropis. 17(2): 1-7.
Verma KK, Prasad S, Kumaresan A, Mohanty TK, Layek SS, Patbandha TK, Chand S. 2014. Characterization of physico-chemical properties of cervical mucus in relation to parity and conception rate in Murrah buffaloes. Vet. World. 7(7): 467-471.