OOCYTE MORPHOMETRY IN BITCHES FROM VARIOUS AGES AND REPRODUCTIVE MATURITY STATUS
Abstract
Dog is one of the animals that has been human’s pet for thousands of years. The aim of this study was to find the morphometry of canine oocyte from various age and reproductive maturity status. In this study, 22 pairs of ovarium samples were collected and stored with Phosphate Buffer Saline (PBS) as transport media. Oocytes were collected using the method of slashing, where multiple slashes were made on the cortex of ovaries and then flushed with NaCl. Petri dish containing NaCl were then observed under the microscope to find the oocyte. Oocyte diameter was obtained by averaging two measurements made perpendicular to each other. If oocytes are obscured by cumulus cells, measurement was taken diagonally. Measurements taken were diameter of oocyte (including zona pellucida) and diameter of ooplasma. Based on statistical analysis of this study using one-way ANOVA, showed oocyte and ooplasma diameters from groups of prepubertal, <1 year old post-pubertal (juvenile), 1-4 years old (mature), and >4 years old (old) are almost indistinguishable. Oocytes from mature dogs had the largest diameter (oocyte = 108,0±27,8µm and ooplasma = 85,7±19,5µm) and was sigfinicantly larger than oocytes from prepubertal dogs (oocyte = 102,5±29,4µm and ooplasma = 84,8±25,4µm). Juvenile dogs had the smallest diameter (oocyte = 94,1±28,9µm and ooplasma = 78,8±25, µm), followed by old dogs (oocyte = 98,5±38,7µm and ooplasma 83,01±35,1µm). Further investigations of oocyte morphometry are needed while focusing more on follicle size, estrus cycle, and oocyte quality.
Downloads
References
Chastant-Maillard S, Viaris De Lesegno C, Chebrout M, Thoumire S, Meylheuc T, Fontbonne A, Chodkiewicz M, Saint-Dizier M, Reynaud K. 2011. The canine oocyte: uncommon features of in vivo and in vitro maturation. Reprod. Fertility Develop. 23(3): 391.
Diagone KV, Vicente WRR, Pacheco MR, Mateus O. 2008. Oocyte morphometry in female dogs (canis familiaris, linnaeus, 1758). Anatomia, Histologia, Embryologia. J. Vet. Med. Series C. 37(2): 81–85.
Fibrianto YH, Pangestiningsih TW, Hanna A, Astuti P, Airin CM, Asyhari, Anindito A, Rachmawati N, Kurniawan K, Wibowo PY. 2008. Kuantitas dan kualitas sel telur anjing lokal dari berbagai stadium estrus. Indon. J. Vet. Sci. 26(1): 10-16.
Greer ML. 2014. Canine reproduction and neonatology. 1st ed. Teton NewMedia. USA.
Guaitolini CRDF, Taffarel MO, Teixeira NS, Sudano MJ, Freitas PMC, Lopes MD, Landin-Alvarenga FDC, de Oliveira CA, Luz MR. 2012. Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing. Theriogenology. 78(3): 576–582.
Haenisch-Woehl A, Kölle S, Neumüller C, Sinowatz F, Braun J. 2003. Morphology of canine cumulus-oocyte complexes in pre-pubertal bitches. Anatomia, Histologia, Embryologia. 32(6): 373–377.
Hewitt D, England G. 1998. The effect of oocyte size and bitch age upon oocyte nuclear maturation in vitro. Theriogenology. 49(5): 957–966.
Hu M, Du Z, Zhou Z, Long H, Ni Q. 2020. Effects of serum and follicular fluid on the in vitro maturation of canine oocytes. Theriogenology. 143: 10–17.
ImageJ. https://imagej.nih.gov/ij/download.html diakses pada tanggal 20 Mei 2022.
Junaidi A. 2021. Reproduksi dan obstetri pada anjing. Gadjah Mada University Press. IDN.
Kindersley D. 2013. The dog encylopedia. First American Edition. DK Publishing. USA.
Kustritz RM. 2009. Clinical canine and feline reproduction: evidence-based answers (1st Ed.). Wiley-Blackwell. USA.
Lee H, Yin X, Jin Y, Kim N, Cho S, Bae I, Kong I. 2008. Germinal vesicle chromatin configuration and meiotic competence is related to the oocyte source in canine. Anim. Reprod. Sci. 103(3–4): 336–347.
Lee HS, Yin XJ, Kong IK. 2006. Sensitivity of canine oocytes to low temperature. Theriogenology. 66(6-7): 1468–1470.
Lunn MO, Wright SJ. 2013. Distinct subtypes of zona pellucida morphology reflect canine oocyte viability and cumulus-oocyte complex quality. Theriogenology. 80(5): 498–506.
No J, Zhao M, Lee S, Ock SA, Nam Y, Hur TY. 2018. Enhanced in vitro maturation of canine oocytes by oviduct epithelial cell co-culture. Theriogenology. 105: 66–74.
Noakes DE, Parkinson TJ, England GCW. 2018. Veterinary reproduction and obstetrics (10th Ed.). Elsevier Health Sciences. DE.
Otoi T, Ooka A, Murakami M, Karja NK, Suzuki T. 2001. Size distribution and meiotic competence of oocytes obtained from bitch ovaries at various stages of the oestrous cycle. Reprod. Fertility Develop. 13(3): 151.
Paulini F, Silva RC, de Paula Rôlo JLJ, Lucci CM. 2014. Ultrastructural changes in oocytes during folliculogenesis in domestic mammals. J. Ovarian Res. 7(1): 1-12.
Pereira LMC, Bicudo SD, Lopes MD. 2012. Oocyte maturation in bitches. Anim. Reprod. 9(3): 205-209.
Pereira LMC, Bicudo SD, Lopes MD. 2012. Oocyte maturation in bitches. Anim. Reprod. 9(3): 205-209.
Reynaud K, Fontbonne A, Saint-Dizier M, Thoumire S, Marnier C, Tahir M, Meylheuc T, Chastant-Maillard S. 2012. Folliculogenesis, ovulation and endocrine control of oocytes and embryos in the dog. Reprod. Domest. Anim. 47: 66–69.
Rocha A, Bastos R, Cunha I, Adona P, Santos J. 2006. Quantity and quality of oocytes recovered from donor bitches of different ages. Theriogenology. 66(6–7): 1465–1467.
Rocha A, Bastos R, Cunha I, Adona P, Santos J. 2006. Quantity and quality of ocytes recovered from donor bitches of different ages. Theriogenology. 66(6–7): 1465–1467.
Saleh W. 2017. Assessment of different methods of bovine oocytes collection, maturation and Invitro fertilization of abattoir specimens. Iraqi J. Vet. Sci. 31(1): 55–65.
Schatten H, Constantinescu GM. 2008. Comparative reproductive biology. Iowa (USA): Blackwell Publishing.
Songsasen N, Fickes A, Pukazhenthi BS, Wildt D. 2009. Follicular morphology, oocyte diameter and localisation of fibroblast growth factors in the domestic dog ovary. Reprod. Domest. Anim. 44: 65–70.
Songsasen N, Wildt D. 2007. Oocyte biology and challenges in developing in vitro maturation systems in the domestic dog. Anim. Reprod. Sci. 98(1–2): 2–22.
Wang GD, Zhai W, Yang HC, Wang L, Zhong L, Liu YH, Fan RX, Yin TT, Zhu CL, Poyarkov AD, Irwin DM, Hytönen MK, Lohi H, Wu CI, Savolainen P, Zhang YP. 2015. Out of southern east asia: the natural history of domestic dogs across the world. Cell Res. 26(1): 21–33.
Zarcula SM, Godja G, Milovanov C, Tulcan C, Cernescu H, Otava G, Bonca G, Ciobota A, Hutu I, Micru C. 2014. Morphological aspects of cumulus-oocyte complexes in different species. Lucrări Ştiinţifice USAMV Iaşi – seria Med. Vet. 57(3/4): 91-97.
Zoppei A, Pinto Neto A, Cattelam J, Martinez A, Trenkel C, Steffens R, Gruchouskei L. 2021. Quantification of multi-oocyte follicles in ovaries of bitches. Arquivo Brasileiro de Med. Vet. Zoot. 73(6): 1269–1277.
