TOTAL PLATE NUMBER OF BACTERIA AND NON-COLIFORM BACTERIA NUMBER IN HEALTHY DOG AND DIARRHEA

  • Indra Manik Pradipta Mahasiswa Kedokteran Hewan, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar Bali, Indonesia, 80234
  • I Gusti Ketut Suarjana Laboratorium Bakteriologi dan Mikologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar Bali, Indonesia, 80234
  • Ketut Tono Pasek Gelgel Laboratorium Bakteriologi dan Mikologi Veteriner, Fakultas Kedokteran Hewan Universitas Udayana, Jl. PB. Sudirman Denpasar Bali, Indonesia, 80234

Abstract

This study aims to determine the total bacterial plate count and the number of non-coliform bacteria in healthy and diarrheal dogs isolated from dog feces at the Denpasar and Badung veterinary clinics. The samples used were fresh feces from healthy dogs and diarrhea with a total of 20 samples (10 healthy and 10 diarrheal). The method of this study was carried out by isolating bacteria on Nutrient Agar media using the pour method for total plate number of bacteria and Eosin Methylene Blue Agar media using the scatter method for non-coliform bacteria. The data obtained were tested using the Independent T Test. The results showed the number in healthy dogs was 3.7 x 107 (CFU/g) for ALTB and 1.5 x 107 (CFU/g) for non-coliform bacteria, while in diarrhea dogs it was 1.5 x 109 (CFU/g) for ALTB and 7.3 x 107 (CFU/g) for non-coliform bacteria. From the results of the study, it was concluded that ALTB and the number of non-coliform bacteria had a significant difference between healthy dogs and diarrhea in which healthy dogs had fewer bacterial colonies than diarrhea dogs which had more bacterial colonies (P?0,05). Further research is needed regarding the effect of non-coliform bacteria from dogs on the surrounding environment.

Downloads

Download data is not yet available.

References

Akhira D, Fahrima Y, Hasan M. 2013. Identifikasi parasit nematoda saluran pencernaan anjing pemburu (canis familiaris) di Kecamatan Lareh Sago Halaban Provinsi Sumatera Barat. J. Med. Vet. 7(1): 42-45.
Amin LZ. 2015. Tatalaksana diare akut. Count. Med. Edu. 42(7): 504-508.
Bambang A, Fatimawali KN. 2014. Analisis cemaran bakteri coliform dan identifikasi Escherichia coli pada air isi ulang dari depot di Kota Manado. J. Ilm. Farm. 3(3): 325-334.
Chlebicz A, Zewska KS. 2018. Campylobacteriosis, salmonellosis, yersiniosis, and listeriosis as zoonotic foodborne diseases: a review. Int. J. Enviro. Res Pub.Health. 15(5): 863.
Dewandaru RA, Indarjulianto S, Yanuartono, Nururrozi A, Purnamaningsih H, Hayati R. 2019. Diare disebabkan infeksi escherichia colipada anjing. J. Trop. Anim. Vet. Sci. 9(2): 38-43.
Febriza MA, Adrian QJ, Sucipto A. 2021. Penerapan ar dalam media pembelajaran klasifikasi bakteri. J. Bio. Edu. UIN. 11(1): 10-18.
Hubbard KS. 2007. Risk of vomiting and dhiarrhoea in dogs. Vet. Rec. 161(22): 755-757.
Kurniati AM. 2016. Mikrobiota saluran cerna. Tinjauan dari Aspek Pemilihan Asupan Makanan. JK Unila. 1(2): 380-384.
Kusumaningsih A. 2010. Beberapa bakteri patogenik penyebab foodborne disease pada bahan pangan asal ternak. Wartazoa. 104.
Luthfia B, Nawfa R. 2011. Pengaruh permeabilisasi sel zymomonas mobilis dan konsentrasi substrat untuk pembentukan etanol secara enzimatik, Pp.1–7.
Rabiulfa P, Rudyanto MD, Sudarmini MW. 2021. Angka lempeng total bakteri pada daging sapi yang dipasarkan keluar Bali. Indon. Med. Vet. 10(1): 12-20.
Rahayu SA, Gumilar MH. 2017. Uji cemaran air minum masyarakat sekitar Margahayu Raya Bandung dengan identifikasi bakteri Escherichia Coli. IJPST. 4(2): 50-56.
Rusli, Amalia F, Dwyana Z. 2018. Potensi bakteri lactobacillus acidophilus sebagai antidiare dan imunomodulator. BIOMA: J. Biol. Makassar. 3(2): 25-30
Sulaksana KA, Suarjana IGT, Besung INK. 2017. Perbandingan jumlah bakteri non-coliform pada feses sapi bali berdasarkan tingkat kedewasaan dan tipe pemeliharan. Bul. Vet. Udayana. 9(2): 139-142.
Tresna S, Rejeki IPS, Wardhani P. 2020. Description of fecal culture result in diarrhea patients due to antibiotic use. Indon. J. Clin. Pathol. Med. Lab. 26(2): 193–197.
Wiliantari PP, Besung IN, Pg KT. 2018. Bakteri coliform dan non coliform yang diisolasi dari saluran pernapasan sapi Bali. Bul. Vet. Udayana. 10(1): 40-41.
Yuswananda NP. 2015. Identifikasi bakteri salmonella sp. pada makanan jajanan di Masjid Fathullah Ciputat. Program Studi Pendidikan Dokter dan Ilmu Kesehatan Universitas Islam Negeri Syarif Hidayatullah.
Published
2022-11-15
How to Cite
PRADIPTA, Indra Manik; SUARJANA, I Gusti Ketut; GELGEL, Ketut Tono Pasek. TOTAL PLATE NUMBER OF BACTERIA AND NON-COLIFORM BACTERIA NUMBER IN HEALTHY DOG AND DIARRHEA. Buletin Veteriner Udayana, [S.l.], p. 430-436, nov. 2022. ISSN 2477-2712. Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/88708>. Date accessed: 16 may 2024. doi: https://doi.org/10.24843/bulvet.2023.v15.i03.p11.
Section
Articles