SENSITIVITY PATTERN OF E. COLI ISOLATED FROM CLOACA OF LAYING HENS WITH DIARRHEA AGAINTS STREPTOMISIN, KANAMISIN AND DOKSISIKLIN
Abstract
The aim of this study was to determine the sensitivity of E. coli bacteria isolated from cloacal swabs of laying hens with diarrhea to streptomycin, kanamycin and doxycycline. A total of 16 samples were used from farms in the villages of Utu (Tabanan), Perean (Tabanan), Demulih (Bangli) and Pasedahan (Karangasem). The sensitivity test of E. coli bacteria used the disc diffusion method from Kirby Bauer. Data analysis was carried out descriptively with the cross tabulation method. The results of 16 isolates of E. coli which were divided into 4 age groups isolated from laying hens cloacal swabs showed a pattern of sensitivity to streptomycin 62.5% resistant, 18.75% intermediates and 18.75% sensitive. The pattern of sensitivity to kanamycin showed an intermediate 6.25% and the sensitivity result was 93.75%. To doxycycline resistance 50%, intermediate 18.75% and sensitive 31.25%. There is an increase in E coli resistance to streptomycin at >4 months of age, while doxycycline at 2-4 months of age and increasing at >4 months of age.
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References
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Bauer AW, Kirby WMM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a stan-dardized single disc method. Am. J. Clin. Pathol. 45(4): 493-496.
Besung INK, Suarjana IGK, Gelgel KTP. 2019. Resistensi antibiotik pada E. coli yang diisolasi dari ayam petelur. Bul. Vet. Udayana. 11(1): 28-32.
Bhaskara IBM, Budiasa K, Gelgel KTP. 2012. Uji kepekaan E. coli sebagai penyebab kolibasilosis pada babi muda terhadap antibiotika oksitetrasiklin, streptomisin, kanamisin dan gentamisin. Indon. Med. Vet. 11(1): 66-75.
Barus DO, Gelgel KTP, Suarjana IGK. 2013. Uji kepekaan bakteri E. coli asal ayam pedaging terhadap antibiotik doksisiklin, gentamisin dan tiamfenikol. Indon. Med. Vet. 2(5): 538-545.
Clinical and Laboratory Standard Institute (CLSI). 2020. Performance standards for antimicrobial susceptibility testing. 30th edition; Thirty Informational Suppletment M100. Clinical and Laboratory Standard Institute. USA
Frans JVP. 2012. Antibiotics. Peranan aminoglikosida dalam mengatasi infeksi serius. MEDICINUS Sci. J. Pharm. Dev. Med. Appl. 25(2): 190-195.
Fransiska. 2019. Ototoksisitas aminoglikosida. Keluwih. 1(1): 37-47.
Handriana IKJ, Gelgel KTP, Suarjana IGK. 2015. Pola kepekaan E. coli yang diisolasi dari feses burung kicau penderita diare terhadap antibiotik sulfametoksazol, ampisilin, dan oksitetrasiklin. Bul. Vet. Udayana. 7(2): 157-163.
Hardjosaputra SL. 2008. Data obat di Indonesia. PT. Muliapurna Jayaterbit, Jakarta. Pp. 299, 444.
Jiang HX, Lu DH, Chen ZL, Wang XM, Chen JR, Liu YH, Liao XP, Liu JH, Zeng ZL. 2009. High prevalence and widespread distribution of multiresistant E. coli Isolates in pigs and poultry in China. Vet. J. 187(1): 99-103.
Masruroh CA, Sudarwanto, Mirnawati B, Latif, Hadri. 2016. Tingkat kejadian E. coli penghasil extended spectrum B-lacktamase pada feses ayam pedaging di Kota Bogor. J. Sain Vet. 34(1): 42-49.
Messaiuml C, Boukhors K, Khelef, Hamdi H. 2013. Antimicrobial susceptibility of Escherichia coli strains isolated from broiler chickens affected by colibacillosis in Setif. J. Microbiol. Res. 7(21): 2668-2672.
Niasono AB, Latif H, Purnawarman T. 2019. Resistensi antibiotik terhadap bakteri Escherichia coli yang diisolasi dari peternakan ayam pedaging di Kabupaten Subang, Jawa Barat. J. Vet. 20(2): 187-195.
Pudjiatmoko. 2014. Manual penyakit unggas. Subdit Pengamatan Penyakit Hewan Direktorat Kesehatan hewan Direktorat jenderal Peternakan dan Kesehatan hewan Kementerian Pertanian, Jakarta. Pp. 103-153.
Purnasari C, Manggau MA, Kasim H. 2018. Studi pengaruh dosis dan lama penggunaan terapi aminoglikosida terhadap fungsi ginjal. Maj. Farm. Farmakol. 22(3): 76-80.
Suardana IW, Utama IH, Putriningsih PAP, Rudyanto MJ. 2014. Uji kepekaan antibiotika Isolat Escherichia coli O157:H7 asal feses ayam. Bul. Vet. Udayana. 6(1): 19-27.
Tyasningsih W, Yutianti Y, Rahmani J, Setiawan B, Harijani N, Budiarto, Effendi MH, Salamah, Witaningru AM. 2020. Antimicrobial resistance profile of Escherichia Coli bakteria collected from cloaca swab of broiler chicken at Surabaya Tradisional Market, Indonesia. Pollut. Res. 40(1): 317-321.
Wattimena JR, Sugiarso NC, Sukandar EY, Widianto MB, Soemardji AA, Setiadi AR. 1987. Farmakodinamika dan terapi antibiotika edisi 1. Gadjah Mada University Press, Yogyakarta. Pp. 128-129.
Werdiningsih S, Yulianti NT, Nurhidayah, Nugraha E, Patriana U, Sari RA, Widyarimbi D, Rusmiati E, Ishrianti NMR. 2014. Profil distribusi beberapa sediaan doksisiklin pada organ/jaringan ayam broiler. Balai Besar Pengujian Mutu dan Sertifikasi Obat Hewan. Bogor. Bul. Pengujian Mutu Obat Hewan. 21(4): 4-7.
Whittam TS, Donnenberg MS. 2011. Pathogenesis and evolution of virulence in enteropathogenic and enterohemorrhagic E. Coli. J. Clin. Invest. 107(5): 539-548.
Wibisono FJ, Sumiarto B, Untari T, Effendi MH, Permatasari DA, Witaningrum AM. 2020. Prevalensi dan analisis faktor risiko multidrug resistance bakteri Escherichia coli pada ayam komersial di Kabupaten Blitar jurnal ilmu peternakan dan veteriner tropis. J. Trop. Anim. Vet. Sci. 10(1): 15-22.
Widyasari EM, Misyetti HAW, Teguh, Witri. 2013. Karakteristik fisikokimia kit kering kanamycin. Indon. J. Nucl. Sci. Technol. 43(2): 117-126.
Zaman S, Hussain M, Nye R, Mehta V, Mamun KT, Hossain N. 2017. A review on antibiotic resistance: alarm bells are ringing. Cureus. 9(6): 1-9.
Published
2022-11-15
How to Cite
DWIPAYANA, I Made Adhi Kusuma; GELGEL, Ketut Tono Pasek; SUARJANA, I Gusti Ketut.
SENSITIVITY PATTERN OF E. COLI ISOLATED FROM CLOACA OF LAYING HENS WITH DIARRHEA AGAINTS STREPTOMISIN, KANAMISIN AND DOKSISIKLIN.
Buletin Veteriner Udayana, [S.l.], p. 423-429, nov. 2022.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/88649>. Date accessed: 15 may 2024.
doi: https://doi.org/10.24843/bulvet.2023.v15.i03.p10.
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