ECOENZYME INHIBITORY TEST AGAINST STAPHYLOCOCCUS SP BACTERIAL GROWTH. ISOLATED FROM THE ECTODERMAL TISSUE OF DOG SKIN
Abstract
Dogs are very susceptible to skin diseases such as dermatitis and bacteria that are often found in cases of dermatitis are Staphylococcus spp. Environmentally friendly herbal medicines and believed to be antimicrobial are ecoenzymes. The ecoenzymes in this study were made from papaya skin (Carica papaya L.), soursop skin (Annona muricata L.), neem leaf (Azadirachta indica A. Juss) and lemongrass (Cymbopogon winterianus Jowitt). This study aims to determine the inhibitory power of ecoenzymes against Staphylococcus spp. bacteria isolated from dog skin ectodermal tissue. This research uses a quantitative method with modification of agar plate diffusion (Kirby Bauer) with well diffusion technique. This study was experimental and used a completely randomized design with 6 treatments, namely ecoenzyme concentrations of 30%, 50%, 70%, 100%, positive control and negative control. Data analysis used ANOVA and continued with the Games-Howell test. The results showed that the ecoenzyme used had inhibition against Staphylococcus spp. bacteria at concentrations of 50%, 70% and 100%.
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References
Akeel RA, Mateen A, Janardhan K, Gupta VC. 2017. Analysis of anti-bacterial and anti oxidative activity of azadiractha indica bark using various solvent extracts. Saudi J. Biol. 24(1): 11-14.
Amin MF, Ariwibowo T, Febria. 2021. Efek antibakteri tumbuhan pepaya (carica papaya L.) Terhadap Porpyromonas Gingivalis. J. Ked.n Gigi Terpadu. 3(1): 86-90.
Arifin LW, Syambarkah A, Purbasari HS, Ria R, Puspita VA. 2009. Introduction of Eco-enzyme to support organic farming in Indonesia. Asian J. Food Agro-Industry. Special issue: 357-358.
Bajwa J. 2016. Canine superficial pyoderma and therapeutic considerations. The Can. Vet. J. 57(2): 204-206.
Boro SEE, Suartha IN, Sudimartini LM. 2018. Efektivitas ekstrak daun mimba terhadap micrococcus luteus yang diisolasi dari anjing penderita dermatitis kompleks. Indon. Med. Vet. 7(5): 588-596.
Dewi MA, Anugrah R, Nurfitri YA. 2016. Uji aktivitas antibakteri ekoenzim terhadap escherchia coli dan shigella dysenteriae. Proc. Sem. Nas. Farmasi. (SNIFA) 2 UNJANI, 978-602-73060-2-8.
Ed-har AA, Widyastuti R, Djajakirana G. 2017. Isolasi dan identifikasi mikroba tanah pendegradasi selulosa dan pektin dari rhizosfer aquilaria malaccensis. Bul. Tanah Lahan. 1(1): 58-64.
Fatoni A, Zusfahair, Lestari P. 2008. Isolasi dan karakterisasi protease ekstraseluler dari bakteri dalam limbah cair tahu. J. Natur Indon.10(2): 83-88.
Hodille E, Badiou C, Bouveyron C, Bes M, Tristan A, Vandenesch F, Lina G, Dumitrescu O. 2018. Clindamycin suppresses virulence expression in inducible clindamycin-resistant staphylococcus aureus strains. Ann. Clin. Microbiol. Antimicrob. 17(38): 1-6.
In YW, Kim JJ, Kim HJ, Oh SW. 2012. Antimikrobial activities of acetic acid, citric acid, and lactic acid againts shigella species. J. Food Safety. 33(1): 79-83.
Lyer V, Raut J, Dasgupta A. 2021. Impact of pH on growth of staphylococcus epidermidis and staphylococcus aureus in vitro. J. Med. Microbiol. 70(9).
Mavani HAK, Tew IM, Wong L, Yew HZ, Mahyuddin A, Ghazali RA, Pow EHN. 2020. Antimicrobial efficacy of fruit peels eco-enzyme against enterpcoccus faecalis: an in vitro study. Int. J. Environ. Res. Pub. Health. 17: 5107.
Medleau L, Hnilica KA. 2006. Small animal dermatology. A Colour Atlas and Therapheutic Guide. Second Edition. Saunders Elseiver. St Louis Missouri.
Medvedova A, Valik L. 2011. Structure and function of food engineering. In Tech, DOI:10.5772/48175.
Morell EA, Baikin DM. 2010. Methicillin-resistant staphylococcus aureus: a pervasive pathogen highlights the need for new antimicrobial developm ent. J. Biol. Med. 83: 223-233.
Nuria MC. 2010. Antibacterial activities from jangkang (homalocladium platycladum (f. muell) bailey) leaves. Mediagro. 6(2): 9-15.
Saepudin SRH, Hanifah S. 2007, Perbandingan penggunaan antibiotika pada pengobatan pasien infeksi saluran kemih yang menjalani rawat inap di salah satu RSUD di Yogyakarta tahun 2004 dan 2006, Fakultas Mipa Jurusan Farmasi, Universitas Islam Indonesia, Yogyakarta.
Sanu EM, Sanam MUE, Tangkonda E. 2015. Uji sensitivitas antibiotika terhadap staphylococcus aureus yang diisolasikan dari luka kulit anjing di Desa Merbaun, Kecamatan Amarasi Barat Kabupaten Kupang. J. Kajian Vet. 3(2): 175-189.
Sapitri A, Mayasari U. 2021. Formulasi sediaan obat kumur dari infusa daun sereh wangi (Cymbopogon winterianus Jowitt ex Bor). J. Health Sain. 2(3): 286-293.
Saraswati HA. 2013. Aktivitas antibbakteri ekstrak etanol daging buah sirsak (annona muricata L.) terhadap staphylococcus aureus, streptococcus pneumoniae, shigella sonnei dan pseudomonas aeruginosa beserta profil kromatografi lapis tipis. Skripsi. Surakarta: Universitas Muhammadiyah Surakarta.
Soedarmo SSP. 2008. Buku ajar infeksi dan pediatri tropis. Ikatan Dokter Anak Indonesia.
Surjowardojo P, Susilorini TE, Benarivo V. 2016. Daya hambat dekok kulit apel malang (malus sylvestris mill) terhadap pertumbuhan escherichia coli dan streptococcus agalactiae penyebab mastitis pada sapi perah. Ternak Trop. 17(1): 11-21.
Susilawati DE. 2019. Staphylococcus penyebab dermatitis pada anjing: isolasi. Identifikasi, dan Uji Sensitivitas Staphylococcus sp. Terhadap Amoxicillin. Skripsi. Yogyakarta: Universitas Gadjah Mada.
Todar K. 2002. Staphylococcus bacteriology at uw-bacteriology 330 home page 1-7.
Toelle NN, Lenda V. 2014. Identifikasi dan karakteristik staphylococcus sp. dan streptococcus sp. dari infeksi ovarium pada ayam petelur komersial. J. Ilmu Ternak. 1(7): 32-37.
Wang N, Weilan AN, Klompas M. 2013. Staphylococcus intermedius infections: case report and literature review. Infect. Dis. Rep. 5(1): e3.
Win YC. 2011. Ecoenzyme activating the earth’s self-healing power. Alih Bahasa: Gan Chiu Har. Malaysia: Summit Print SDN. BHD; 6, 8, 9-14.
Amin MF, Ariwibowo T, Febria. 2021. Efek antibakteri tumbuhan pepaya (carica papaya L.) Terhadap Porpyromonas Gingivalis. J. Ked.n Gigi Terpadu. 3(1): 86-90.
Arifin LW, Syambarkah A, Purbasari HS, Ria R, Puspita VA. 2009. Introduction of Eco-enzyme to support organic farming in Indonesia. Asian J. Food Agro-Industry. Special issue: 357-358.
Bajwa J. 2016. Canine superficial pyoderma and therapeutic considerations. The Can. Vet. J. 57(2): 204-206.
Boro SEE, Suartha IN, Sudimartini LM. 2018. Efektivitas ekstrak daun mimba terhadap micrococcus luteus yang diisolasi dari anjing penderita dermatitis kompleks. Indon. Med. Vet. 7(5): 588-596.
Dewi MA, Anugrah R, Nurfitri YA. 2016. Uji aktivitas antibakteri ekoenzim terhadap escherchia coli dan shigella dysenteriae. Proc. Sem. Nas. Farmasi. (SNIFA) 2 UNJANI, 978-602-73060-2-8.
Ed-har AA, Widyastuti R, Djajakirana G. 2017. Isolasi dan identifikasi mikroba tanah pendegradasi selulosa dan pektin dari rhizosfer aquilaria malaccensis. Bul. Tanah Lahan. 1(1): 58-64.
Fatoni A, Zusfahair, Lestari P. 2008. Isolasi dan karakterisasi protease ekstraseluler dari bakteri dalam limbah cair tahu. J. Natur Indon.10(2): 83-88.
Hodille E, Badiou C, Bouveyron C, Bes M, Tristan A, Vandenesch F, Lina G, Dumitrescu O. 2018. Clindamycin suppresses virulence expression in inducible clindamycin-resistant staphylococcus aureus strains. Ann. Clin. Microbiol. Antimicrob. 17(38): 1-6.
In YW, Kim JJ, Kim HJ, Oh SW. 2012. Antimikrobial activities of acetic acid, citric acid, and lactic acid againts shigella species. J. Food Safety. 33(1): 79-83.
Lyer V, Raut J, Dasgupta A. 2021. Impact of pH on growth of staphylococcus epidermidis and staphylococcus aureus in vitro. J. Med. Microbiol. 70(9).
Mavani HAK, Tew IM, Wong L, Yew HZ, Mahyuddin A, Ghazali RA, Pow EHN. 2020. Antimicrobial efficacy of fruit peels eco-enzyme against enterpcoccus faecalis: an in vitro study. Int. J. Environ. Res. Pub. Health. 17: 5107.
Medleau L, Hnilica KA. 2006. Small animal dermatology. A Colour Atlas and Therapheutic Guide. Second Edition. Saunders Elseiver. St Louis Missouri.
Medvedova A, Valik L. 2011. Structure and function of food engineering. In Tech, DOI:10.5772/48175.
Morell EA, Baikin DM. 2010. Methicillin-resistant staphylococcus aureus: a pervasive pathogen highlights the need for new antimicrobial developm ent. J. Biol. Med. 83: 223-233.
Nuria MC. 2010. Antibacterial activities from jangkang (homalocladium platycladum (f. muell) bailey) leaves. Mediagro. 6(2): 9-15.
Saepudin SRH, Hanifah S. 2007, Perbandingan penggunaan antibiotika pada pengobatan pasien infeksi saluran kemih yang menjalani rawat inap di salah satu RSUD di Yogyakarta tahun 2004 dan 2006, Fakultas Mipa Jurusan Farmasi, Universitas Islam Indonesia, Yogyakarta.
Sanu EM, Sanam MUE, Tangkonda E. 2015. Uji sensitivitas antibiotika terhadap staphylococcus aureus yang diisolasikan dari luka kulit anjing di Desa Merbaun, Kecamatan Amarasi Barat Kabupaten Kupang. J. Kajian Vet. 3(2): 175-189.
Sapitri A, Mayasari U. 2021. Formulasi sediaan obat kumur dari infusa daun sereh wangi (Cymbopogon winterianus Jowitt ex Bor). J. Health Sain. 2(3): 286-293.
Saraswati HA. 2013. Aktivitas antibbakteri ekstrak etanol daging buah sirsak (annona muricata L.) terhadap staphylococcus aureus, streptococcus pneumoniae, shigella sonnei dan pseudomonas aeruginosa beserta profil kromatografi lapis tipis. Skripsi. Surakarta: Universitas Muhammadiyah Surakarta.
Soedarmo SSP. 2008. Buku ajar infeksi dan pediatri tropis. Ikatan Dokter Anak Indonesia.
Surjowardojo P, Susilorini TE, Benarivo V. 2016. Daya hambat dekok kulit apel malang (malus sylvestris mill) terhadap pertumbuhan escherichia coli dan streptococcus agalactiae penyebab mastitis pada sapi perah. Ternak Trop. 17(1): 11-21.
Susilawati DE. 2019. Staphylococcus penyebab dermatitis pada anjing: isolasi. Identifikasi, dan Uji Sensitivitas Staphylococcus sp. Terhadap Amoxicillin. Skripsi. Yogyakarta: Universitas Gadjah Mada.
Todar K. 2002. Staphylococcus bacteriology at uw-bacteriology 330 home page 1-7.
Toelle NN, Lenda V. 2014. Identifikasi dan karakteristik staphylococcus sp. dan streptococcus sp. dari infeksi ovarium pada ayam petelur komersial. J. Ilmu Ternak. 1(7): 32-37.
Wang N, Weilan AN, Klompas M. 2013. Staphylococcus intermedius infections: case report and literature review. Infect. Dis. Rep. 5(1): e3.
Win YC. 2011. Ecoenzyme activating the earth’s self-healing power. Alih Bahasa: Gan Chiu Har. Malaysia: Summit Print SDN. BHD; 6, 8, 9-14.
Published
2023-03-04
How to Cite
SINTHALAROSA, Margaretha Dhea; SUARTHA, I Nyoman; SUDIPA, Putu Hendrywaesa.
ECOENZYME INHIBITORY TEST AGAINST STAPHYLOCOCCUS SP BACTERIAL GROWTH. ISOLATED FROM THE ECTODERMAL TISSUE OF DOG SKIN.
Buletin Veteriner Udayana, [S.l.], p. 278-285, mar. 2023.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/87207>. Date accessed: 24 apr. 2024.
doi: https://doi.org/10.24843/bulvet.2023.v15.i02.p14.
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