GROWTH INHIBITION TEST OF ECO-ENZYME AGAINTS STREPTOCOCCUS SPP. ISOLATED FROM THE ECTODERMAL TISSUE OF DOG’S SKIN
Abstract
Dogs skin are susceptible to skin diseases cause by bacterial infection. Although Streptococcus spp. is a component of the normal flora of the skin, streptococcal infection can cause diseases in dogs such as abscesses, local infections, and life-threatening septicemia. Eco-enzyme fermentation products are suspected to have high antibacterial activity due to the content of acetic acid. This study aims to determine the antibacterial activity of eco-enzyme in inhibiting the growth of Streptococcus spp. using the agar well diffusion method with a completely randomized design experiment of 6 treatments and 4 trials which include eco-enzyme with concentrations of 30%, 50%, 70%, 100%, aquadest as the negative control, and Penicillin G as the positive control. The parameter measured is the diameter of the inhibition zone formed. The data were then analyzed with Analysis of Variance (ANOVA). The results of the study showed that the eco-enzyme with concentrations of 30%, 70%, and 100% were able to inhibit the growth of Streptococcus spp.
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References
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Suprianto. 2008. Potensi ekstrak sereh wangi (Cymbopongon nardus L.) sebagai anti Streptococcus mutans. Dissertations. Bogor: Institut Pertanian Bogor.
Surjowardojo P, Susilorini TE, Benarivo V. 2016. Daya hambat dekok kulit apel malang (Malus sylvestris Mill) terhadap pertumbuhan Escherichia coli dan Streptococcus agalactiae penyebab mastitis pada sapi perah. Ternak Trop. 17(1): 11-21.
Arifin WL, Syambarkyah, Argya, Purbasari, Sutsuga H, Ria, Rizkita, Puspita VA. 2009. Introduction of Eco-enzyme to support organic farming in Indonesia. Asian J. Food Agro-Industry. 357-358.
Aviany HB, Pujiyanto S. 2020. Analisis efektivitas probiotik di dalam produk kecantikan sebagai antibakteri terhadap bakteri Staphylococcus epidermidis. Berkala Bioteknol. 3(2): 25-29.
Chen J. 2021. Cultivation media for bacteria. https://learn.chm.msu.edu/vibl/content/differential/
Clinical and Laboratory Standards Institute. 2020. Performance standards for antimicrobial susceptibility testing. CLSI eCLIPSE 30th Ed.
Cortesia C, Vilcheze C, Bernut A, Contreras W, Gomez K, Waard J, Jacobs WR, Kremer L, Takiff H. 2014. Acetic acid, the active component of vinegar, is an effective tuberculocidal disinfectant. mBio. 5(2): e00013-14.
Damayanti SS, Komala O, Effendi EM. 2018. Identifikasi bakteri dari pupuk organik cair isi rumen sapi. Ekologia. 18(2): 63-71.
Dewi MA, Anugrah R, Nurfitri YA. 2017. Uji aktivitas antibakteri ekoenzim terhadap Escherichia coli dan Shigella dysenteriae. Dissertations. Cimahi: Universitas Jendral Achmad Yani.
Fitriasari PD, Amalia N, Farkhiyah S. 2020. Isolasi dan uji kompatibilitas bakteri hidrolitik dari tanah tempat pemrosesan akhir Talangagung, Kabupaten Malang. Berita Biol. 19(2): 151-152.
Henaulu AH, Kaihena M. 2020. Potensi antibakteri ekstrak etanol daun kecipir (Psophocarpus tetragonolobus (L.)DC) terhadap pertumbuhan Escherichia coli dan Staphylococcus aureus in vitro. Biofaal J. 1(1): 44-54.
Hermawan, Hana A. 2007. Pengaruh ekstrak daun sirih (piper betle 1) terhadap pertumbuhan Staphylococcus aureus dan Eschericia coli dengan metode difusi disk. Dissertations. Surabaya: Universitas Erlangga.
Kundukad B, Schussman M, Yang K, Seviour T, Yang L, Rice SA, Kjelleberg S, Doyle PS. 2017. Mechanistic action of weak acid drugs on biofilms. Sci. Rep. 7: 4783.
Lamm CGAC, Ferguson TW, Lehenbauer BC, Love. 2010. Streptococcal infection in dogs a retrospective study of 393 cases. Vet. Pathol. 47(3): 387-395.
Mavani HAK, Tew IM, Wong L, Yew HZ, Mahyuddin A, Ghazali RA, Pow EHN. 2020. Antimicrobial ecacy of fruit peels eco-enzyme against enterococcus faecalis: an in vitro study. Int. J. Environ. Res. Pub. Health 2020. 17: 1-12.
Medleau L, Hnilica KA. 2006. Small animal dermatology. A Colour Atlas and Therapheutic Guide. Second Edition. Saunders Elseiver. UK. Pp. 25-61.
Muljono P, Fatimawali, Manampiring AE. 2016. Uji aktivitas antibakteri ekstrak daun mayana jantan (Coleus atropurpureus Benth) terhadap pertumbuhan bakteri Streptococcus Sp. dan Pseudomonas Sp. J. e-Biomed. 4(1): 164-172.
Rahayu S. 2015. Deteksi streptococcus agalactiae penyebab mastitis subklinis pada sapi perah di Kecamatan Cendana Kabupaten Enrekang. Dissertations. Makassar: Universitas Hasanuddin.
Rahma RPA, Bahar M, Harjono Y. 2017. Uji daya hambat filtrat zat metabolit Lactobacillus plantarum terhadap pertumbuhan Shigella dysenteriae secara In Vitro. J. Ilmiah Biol. 5(1): 32-41.
Rastina R, Sudarwanto M, Wientarsih I. 2015. Aktivitas antibakteri ekstrak etanol daun kari (Murraya koenigii) terhadap Staphylococcus aureus, Escherichia coli, dan Pseudomonas sp. J. Ked. Hewan. 9(2): 185-188.
Rochyani N, Utpalasari RL, Dahliana I. 2020. Analisis hasil konversi eco enzyme menggunakan nenas (Ananas comosus) dan pepaya (Carica papaya L.). J. Redoks. 5(2): 135-136.
Rusdianasari R, Syakdani A, Zaman M, Sari FF, Nasyta NP, Amalia R. 2021. Production of disinfectant by utilizing Eco-enzyme from fruit peels waste. Int. J. Res. Voc. Stud. 1(3): 01-07.
Saepudin, Sulistiawan RH, Hanifah S. 2007. Perbandingan penggunaan antibiotika pada pengobatan pasien infeksi saluran kemih yang menjalani rawat inap di salah satu RSUD di Yogyakarta Tahun 2004 dan 2006. Dissertations. Yogyakarta: Universitas Islam Indonesia.
Sizu B, Shah NP. 2003. Effects of pH, temperature, supplementation with whey protein concentrate, and adjunct cultures on the production of exopolysaccharides by Streptococcus thermophilus 1275. J. Dairy Sci. 86(11): 3405-3415.
Suprianto. 2008. Potensi ekstrak sereh wangi (Cymbopongon nardus L.) sebagai anti Streptococcus mutans. Dissertations. Bogor: Institut Pertanian Bogor.
Surjowardojo P, Susilorini TE, Benarivo V. 2016. Daya hambat dekok kulit apel malang (Malus sylvestris Mill) terhadap pertumbuhan Escherichia coli dan Streptococcus agalactiae penyebab mastitis pada sapi perah. Ternak Trop. 17(1): 11-21.
Published
2022-06-22
How to Cite
WELFALINI, Sheira Tannia; SUARTHA, I Nyoman; SUDIPA, Putu Henrywaesa.
GROWTH INHIBITION TEST OF ECO-ENZYME AGAINTS STREPTOCOCCUS SPP. ISOLATED FROM THE ECTODERMAL TISSUE OF DOG’S SKIN.
Buletin Veteriner Udayana, [S.l.], p. 169-176, june 2022.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/86815>. Date accessed: 21 nov. 2024.
doi: https://doi.org/10.24843/bulvet.2023.v15.i02.p02.
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