BACTERIAL SENSITIVITY TEST KLEBSIELLA SPP. ISOLATED FROM KINTAMANI DOG DIARRHEA AGAINST ANTIBIOTICS
Abstract
This study aims to determine the pattern of sensitivity of bacteria Klebsiella spp. isolated from kintamani dogs with diarrhea. The sample of this study were 4 isolates of Klebsiella spp. isolated from kintamani dogs with diarrhea. The method used in this study is the Kirby-Bauer disc diffusion method in duplicate using the antibiotics Kanamycin, Sulfamethoxazole-trimethoprim, and Streptomycin. The data obtained were expressed descriptively qualitatively by measuring the antibiotic inhibition zone formed. The results showed that isolates of Klebsiella spp. 75% intermediate (3 isolates) and 25%(1 isolate) sensitive to Kanamycin, all samples sensitive to Sulfamethoxazole-trimethoprim and 75% (3 isolates) sample intermediate and 25% (1 isolate) sensitive to Streptomycin.
Downloads
Download data is not yet available.
References
Abebe GM. 2020. Detection of biofilm formation and antibiotic resistance in klebsiella oxytoca and klebsiella pneumoniae from animal origin foods. Int. J. Microbiol. Biotechnol. 5(3): 120-130.
Anholt RM, Berezowski J, Ribble CS, Russell ML, Stephen C. 2014. Using informatics and the electronic medical record to describe antimicrobial use in the clinical management of diarrhea cases at 12 companion animal practices. PLoS One. 9(7): e103190.
Clinical Laboratory Standards Institute (CLSI). 2020. Performance standards for antimicrobial susceptibility testing. 30th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute
Dewandaru RA, Indarjulianto S, Yanuartono, Nururrozi A, Purnamaningsih A, Hayati R. 2019. Diare disebabkan infeksi escherichia coli pada anjing. J. Trop. Anim. Vet. Sci. 9(2): 38-43.
Duse A, Persson-Waller K, Pedersen K. 2021. Microbial aetiology, antibiotic susceptibility and pathogen specific risk factors for udder pathogens from clinical mastitis in dairy cows. Animals. 11(7): 1-17.
Etebu E, Arikekpar I. 2016. Antibiotics: Classification and mechanisms of action with emphasis on molecular perspectives. Int. J. Appl. Microbiol. Biotechnol. Res. 4: 90-101.
Evayana M, Dwinata IM, Puja IK. 2017. Prevalensi infeksi cacing toxocara canis pada anjing kintamani di Desa Sukawana, Kecamatan Kintamani, Kabupaten Bangli, Bali. Indon. Med. Vet. 6(2): 115-123.
German AJ, Halladay LJ, Noble PJM. (2010). First-choice therapy for dogs presenting with diarrhoea in clinical practice. Vet. Rec. 167(21): 810–814.
Gómez-Beltrán DA, Schaeffer DJ, Ferguson DC, Monsalve LK, Villar D. 2021. Antimicrobial prescribing practices in dogs and cats by colombian veterinarians in the city of medellin. Vet. Sci. 8(5): 1-11.
Grosset JH, Singer T. (2013). Streptomycin. Brenner’s Encyclopedia of Genetics. 5: 568–569.
Hansur L, Ugi D, Hamball H. 2019. Uji kepekaan bakteri asam laktat kandidat probiotik terhadap antibiotik kanamisin, oleandomisin, dan polimiksin B. eJKI. 7(1): 61-65.
Hossain S, De Silva BCJ, Dahanayake PS, Heo G. 2019. Phylogenetic relationships, virulence and antimicrobial resistance properties of Klebsiella spp. isolated from pet turtles in Korea. Let. Appl. Microbiol. 70: 71-78.
Koneman EW, Procop GW, Church DL, Hall GS, Janda WM, Woods GL. 2017. Koneman's color atlas and textbook of diagnostic microbiology. Philadelphia: Lippincott Williams & Wilkins.
Krause KM, Serio AW, Kane TR, Connolly LE. (2016). Aminoglycosides: an overview. Cold Spring Harbor Perspectives in Med. 6(6): 1-18.
Majewski M, Józefiak A, Kimsa-Furdzik M, Dziubdziela L, Hudak-Nowak M, Wilczyński J, Anusz K. 2021. Antimicrobial resistance of Escherichia coli and Klebsiella spp. conventionally sampled from factory-farmed chickens clinical submissions. Ann. Agric. Environ. Med. 28(2): 271–276.
Marks SL, Kather EJ. 2003. Bacterial-associated diarrhea in the dog: a critical appraisal. Small Anim. Pract. 33(5): 1029–1060.
Marks SL, Rankin SC, Byrne BA, Wesse, JS. 2011. Enteropathogenic bacteria in dogs and cats: diagnosis, epidemiology, treatment, and control. J. Vet. Intern. Med. 25(6):1195–1209.
Munirah, Rasidah E, Melani N, Zakiah, Nasir M. 2018. Uji aktivitas antibakteri etanol daun ketapang (terminalia catappa l.) warna hijau dan warna merah serta kombinasinya. Indon. J. Pharm. Nat. Prod. 1(2): 8-13.
Mustika OC, Pinatih KJP, Suardana IW. 2015. Uji kepekaan Escherichia coli O157:H7 feses sapi di Kecamatan Kuta Selatan Badung Bali terhadap antibiotik. Indon. Med. Vet. 4(4): 342-350.
Nazara AL, Suarjana IGK, Tono KPG. 2022. Bakteri klebsiella sp. asal babi penderita porcine respiratory disease complex resistan terhadap ampicillin dan peka sulfamethoxazole-trimethoprim dan kanamycin. Indon. Med. Vet. 11(1): 66-75.
Pilla R, Suchodolski JS. 2019. The role of the canine gut microbiome and metabolome in health and gastrointestinal disease. Front. Vet. Sci. 6(498): 1-12.
Puja IK. 2007. Anjing kintamani maskot fauna Kabupaten Bangli. Penerbit Universitas Udayana Bali.
Purba DA, Tono KPG, Suarjana IGK. 2022. Uji kepekaan streptococcus spp.yang diisolasi dari penyakit saluran pernapasan kompleks babi terhadap kanamycin, streptomycin dan doxyxycline. Bul. Vet. Udayana. 14(3): 202-209.
Samanta I, Bandyopadhyay S. 2020. Klebsiella. Antimicrobial Resistance in Agriculture. Elsevier Pp. 153–169.
Santosa PE. 2016. Efektivitas berbagai preparat antibiotika terhadap kasus omphalitis pada ayam broiler. J. Ilmiah Pet. Terpadu. 4(4): 319 – 322.
Sharif NM, Sreedevi B, Chaitanya RK, Sreenivasulu D. 2017. Beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh, India. Vet. World. 10(8): 950-954.
Shi Y, Yang H, Chu M, Niu X, Huo X, Gao Y, Zeng J, Zhang T, Li YG, Outi K, Lou K, Li X, Dang, Li C. 2020. Klebsiella. Beneficial Microbes in Agro-Ecol. Pp. 233–257.
Upmanyu N, Malviya VN. (2020). Antibiotics: mechanisms of action and modern challenges. Microorganisms for Sustainable Environment and Health. Pp. 367–382.
Widyasari AM, Misyetti TH, Ambar, Nuraeni. 2013. Karakteristik fisikokimia kit kering kanamycin. J. Sains Teknol. Nuklir Indon. 43(2): 117-126.
Zhang X, Wang J, Wu Q, Li L, Wang Y, Yang H. (2019). Determination of kanamycin by high performance liquid chromatography. Molecules. 24(10): 1902.
Anholt RM, Berezowski J, Ribble CS, Russell ML, Stephen C. 2014. Using informatics and the electronic medical record to describe antimicrobial use in the clinical management of diarrhea cases at 12 companion animal practices. PLoS One. 9(7): e103190.
Clinical Laboratory Standards Institute (CLSI). 2020. Performance standards for antimicrobial susceptibility testing. 30th ed. CLSI supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute
Dewandaru RA, Indarjulianto S, Yanuartono, Nururrozi A, Purnamaningsih A, Hayati R. 2019. Diare disebabkan infeksi escherichia coli pada anjing. J. Trop. Anim. Vet. Sci. 9(2): 38-43.
Duse A, Persson-Waller K, Pedersen K. 2021. Microbial aetiology, antibiotic susceptibility and pathogen specific risk factors for udder pathogens from clinical mastitis in dairy cows. Animals. 11(7): 1-17.
Etebu E, Arikekpar I. 2016. Antibiotics: Classification and mechanisms of action with emphasis on molecular perspectives. Int. J. Appl. Microbiol. Biotechnol. Res. 4: 90-101.
Evayana M, Dwinata IM, Puja IK. 2017. Prevalensi infeksi cacing toxocara canis pada anjing kintamani di Desa Sukawana, Kecamatan Kintamani, Kabupaten Bangli, Bali. Indon. Med. Vet. 6(2): 115-123.
German AJ, Halladay LJ, Noble PJM. (2010). First-choice therapy for dogs presenting with diarrhoea in clinical practice. Vet. Rec. 167(21): 810–814.
Gómez-Beltrán DA, Schaeffer DJ, Ferguson DC, Monsalve LK, Villar D. 2021. Antimicrobial prescribing practices in dogs and cats by colombian veterinarians in the city of medellin. Vet. Sci. 8(5): 1-11.
Grosset JH, Singer T. (2013). Streptomycin. Brenner’s Encyclopedia of Genetics. 5: 568–569.
Hansur L, Ugi D, Hamball H. 2019. Uji kepekaan bakteri asam laktat kandidat probiotik terhadap antibiotik kanamisin, oleandomisin, dan polimiksin B. eJKI. 7(1): 61-65.
Hossain S, De Silva BCJ, Dahanayake PS, Heo G. 2019. Phylogenetic relationships, virulence and antimicrobial resistance properties of Klebsiella spp. isolated from pet turtles in Korea. Let. Appl. Microbiol. 70: 71-78.
Koneman EW, Procop GW, Church DL, Hall GS, Janda WM, Woods GL. 2017. Koneman's color atlas and textbook of diagnostic microbiology. Philadelphia: Lippincott Williams & Wilkins.
Krause KM, Serio AW, Kane TR, Connolly LE. (2016). Aminoglycosides: an overview. Cold Spring Harbor Perspectives in Med. 6(6): 1-18.
Majewski M, Józefiak A, Kimsa-Furdzik M, Dziubdziela L, Hudak-Nowak M, Wilczyński J, Anusz K. 2021. Antimicrobial resistance of Escherichia coli and Klebsiella spp. conventionally sampled from factory-farmed chickens clinical submissions. Ann. Agric. Environ. Med. 28(2): 271–276.
Marks SL, Kather EJ. 2003. Bacterial-associated diarrhea in the dog: a critical appraisal. Small Anim. Pract. 33(5): 1029–1060.
Marks SL, Rankin SC, Byrne BA, Wesse, JS. 2011. Enteropathogenic bacteria in dogs and cats: diagnosis, epidemiology, treatment, and control. J. Vet. Intern. Med. 25(6):1195–1209.
Munirah, Rasidah E, Melani N, Zakiah, Nasir M. 2018. Uji aktivitas antibakteri etanol daun ketapang (terminalia catappa l.) warna hijau dan warna merah serta kombinasinya. Indon. J. Pharm. Nat. Prod. 1(2): 8-13.
Mustika OC, Pinatih KJP, Suardana IW. 2015. Uji kepekaan Escherichia coli O157:H7 feses sapi di Kecamatan Kuta Selatan Badung Bali terhadap antibiotik. Indon. Med. Vet. 4(4): 342-350.
Nazara AL, Suarjana IGK, Tono KPG. 2022. Bakteri klebsiella sp. asal babi penderita porcine respiratory disease complex resistan terhadap ampicillin dan peka sulfamethoxazole-trimethoprim dan kanamycin. Indon. Med. Vet. 11(1): 66-75.
Pilla R, Suchodolski JS. 2019. The role of the canine gut microbiome and metabolome in health and gastrointestinal disease. Front. Vet. Sci. 6(498): 1-12.
Puja IK. 2007. Anjing kintamani maskot fauna Kabupaten Bangli. Penerbit Universitas Udayana Bali.
Purba DA, Tono KPG, Suarjana IGK. 2022. Uji kepekaan streptococcus spp.yang diisolasi dari penyakit saluran pernapasan kompleks babi terhadap kanamycin, streptomycin dan doxyxycline. Bul. Vet. Udayana. 14(3): 202-209.
Samanta I, Bandyopadhyay S. 2020. Klebsiella. Antimicrobial Resistance in Agriculture. Elsevier Pp. 153–169.
Santosa PE. 2016. Efektivitas berbagai preparat antibiotika terhadap kasus omphalitis pada ayam broiler. J. Ilmiah Pet. Terpadu. 4(4): 319 – 322.
Sharif NM, Sreedevi B, Chaitanya RK, Sreenivasulu D. 2017. Beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh, India. Vet. World. 10(8): 950-954.
Shi Y, Yang H, Chu M, Niu X, Huo X, Gao Y, Zeng J, Zhang T, Li YG, Outi K, Lou K, Li X, Dang, Li C. 2020. Klebsiella. Beneficial Microbes in Agro-Ecol. Pp. 233–257.
Upmanyu N, Malviya VN. (2020). Antibiotics: mechanisms of action and modern challenges. Microorganisms for Sustainable Environment and Health. Pp. 367–382.
Widyasari AM, Misyetti TH, Ambar, Nuraeni. 2013. Karakteristik fisikokimia kit kering kanamycin. J. Sains Teknol. Nuklir Indon. 43(2): 117-126.
Zhang X, Wang J, Wu Q, Li L, Wang Y, Yang H. (2019). Determination of kanamycin by high performance liquid chromatography. Molecules. 24(10): 1902.
Published
2023-03-06
How to Cite
ANANTA KUSUMA, Anak Agung Gede Agung; SUARJANA, I Gusti Ketut; GELGEL, Ketut Tono Pasek.
BACTERIAL SENSITIVITY TEST KLEBSIELLA SPP. ISOLATED FROM KINTAMANI DOG DIARRHEA AGAINST ANTIBIOTICS.
Buletin Veteriner Udayana, [S.l.], p. 205-210, mar. 2023.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/86801>. Date accessed: 21 nov. 2024.
doi: https://doi.org/10.24843/bulvet.2023.v15.i02.p07.
Issue
Section
Articles
