NEWCASTLE DISEASE MATERNAL ANTIBODIES DETECTION IN BROILERS
Abstract
Nevertheless, it is often found that the after vaccination antibody titers is not as expected due to the existance of maternal antibodies. A passive maternal antibodies can inhibit formation of imunoglobulin and neutralize the antigen vaccines. According to that above mentioned explanation, this research aims to detect the maternal antibodies of Newcastle Disease in broilers to carefully identify when the chicken should be vaccinated. As the main object of study, 10 unvaccinated broilers would be included in this research. These samples would be grouped by 3 different times of collecting blood, namely at the age of 7th, 14th, and 21st days. Blood sampling is subsequently carried out through branchialis vein that concludes 10 serum samples from each day. The respective samples will be serologically examined by conducting the haemaglutination (HA) and haemaglutination inhibition (HI) analysis to generate the antibody titers. All the data produced in this research will statistically processed by using descriptive statistical and regression tests on the SPSS software. The results showed a decrease in the maternal antibody titer of Newcastle Disease in broilers aged 7 days with a titer of 6.1 HI log 2, to 2.8 HI log 2 at 14 days of age, and 1.8 HI log 2 at 21 days of age. The Newcastle Disease vaccination program is most appropriate when the broiler is 10 days old because the maternal antibody titer is already at the positive threshold, namely 4 HI log 2.
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References
Alexander, DJ, Senne DA. 2008. Newcastle disease, other avian paramyxovirus, and pneumovirus infection. Dalam: Disease of the Poultry, 12 Edition. Saif, Y.M. Blackwell Publishing, Iowa. Hal. 75-92.
Ali MZ, Hasan B. 2018. Follow up of maternally derived antibodies titer against economically important viral diseases in chicken. Poult. Sci. J. 6(2): 149-154.
Jalil MA, Samad MA, Islam MT. 2009. Evaluation of maternally derived antibodies against Newcastle disease virus and its effect on vaccination in broiler chicks. Bangladesh J. Vet. Med. 7(2): 296-302.
Callahan GN, Yates RM, Warren AL. 2014. Basic veterinary immunology. University Press of Colorado.
Darmawi, Fakhrurrazi, Wiliana, Maryulia D, Mahdi A. Faisal J, Zakiah HM. 2015. Deteksi antibodi serum ayam kampung (gallus domesticus) terhadap virus newcastle disease di Kota Banda Aceh. J. Med. Vet. 9(1): 5-8.
Gharaibeh S, Mahmoud K. 2013. Decay of maternal antibodies in broiler chickens. Poult. Sci. 92(9): 2333-2336.
Hamal KR, Burgess SC, Pevzner IY, Erf GF. 2006. Maternal antibody transfer from dams to their egg yolks, egg whites, and chicks in meat lines of chickens. Poult. Sci. 85(8): 1364-1372.
OIE. 2012. Newcastle disease chapter 2.3.14. www.oie.int [Diakses pada 19 Desember 2021]
Paniago M. 2007. Vaccination Againts Newcastle Disease in the Hatcheries. Ceva Animal Health Asia. Selangor. www.thepoultrysite.com.
Kencana GAY, Kardena IM. 2011. Gross pathological observation of acute Newcastle Disease in domestic chicken. Proc. Seminar Internasioanal Perhimpunan Mikrobiologi Indonesia (PERMI) dan International Union of Microbiological Societies (IUMS) di Denpasar, 22-24 Juni 2011.
Kencana GAY, Suartha N, Simbolon MP, Handayani AN, Ong S, Syamsidar AK. 2015. Respons antibodi terhadap penyakit tetelo pada ayam yang divaksin tetelo dan tetelo-flu burung. J. Vet. 16(2): 283-290.
Kencana GAY, Suartha IN, Paramita NMAS, Handayani AN. 2016. Vaksin kombinasi Newcastle Disease dengan Avian Influenza memicu imunitas protektif pada ayam petelur terhadap penyakit tetelo dan flu burung. J. Vet. 17(2): 257-264.
Niewiesk S. 2014. Maternal antibodies: clinical significance, mechanism of interference with immune responses, and possible vaccination strategies. Front. Immunol. 5: 446.
Nurkholis DR, Tantalo S, Santosa PE. 2014. Pengaruh pemberian kunyit dan temulawak melalui air minum terhadap titer antibody AI, IBD, dan ND pada Broiler. J. Ilm. Pet. Terpadu. 2(2): 37-43.
Nursahara A. 2019. Pengaruh pemberian jati cina (cassia angustifolia) sebagai imunostimulan titer antibodi pada puyuh yang divaksin ND (Newcastle disease). Kumpulan Karya Ilmiah Mahasiswa Fakultas sains dan Tekhnologi. 1(1): 422-422.
Sampurna IP, Nindhia TS. 2017. Metodologi penelitian dan karya ilmiah. Denpasar: Fakultas Kedokteran Hewan Universitas Udayana.
Saputro B, Santosa PE, Kurtini T. 2014. Pengaruh cara pemberian vaksin nd live pada broiler terhadap titer antibodi, jumlah sel darah merah dan sel darah putih. J. Ilm. Pet. Terpadu. 2(3).
Sa'idu L, Abdu PA. 2008. Outbreak of Viscerotropic Velogenic form of Newcastle dis-ease in vaccinated six weeks old pullets. Sokoto J. Vet. Sci. 7(1): 37-40.
Suardana IBK, Dewi NMRK, Mahardika IGNK. 2009. Respon imun itik bali terhadap berbagai dosis vaksin avian influenza H5N1. J. Vet. 10(3): 150-155.
Szenborn L, Tischer A, Pejcz J, Rudkowski Z, Wojcik M. 2003. Passive acquired immunity against measles in infants born to naturally infected and vaccinated mothers. Med. Sci. Monit.. 9(12): 541-546.
Tizard IR. 2017. Veterinary Immunology-E-Book. Elsevier Health Sciences.
Utomo HR, Setiyawan H, Santoso SI. 2015. Analisis profitabilitas usaha peternakan ayam broiler dengan pola kemitraan di Kecamatan Limbangan Kabupaten Kendal profitability analysis of livestock broiler business with partnership pattern in the District Limbangan Kendal. Anim. Agric. J. 4(1): 7-14.
