GROSS PATHOLOGY AND HISTOPATHOLOGY DESCRIPTION OF NERVOUS SYSTEM OF BALI DUCK AFTER EXPERIMENTAL INFECTION WITH AVIAN ORTHOAVULAVIRUS 1 TABANAN-1/ARP/2017 ISOLATE
Abstract
The present study aims to determine the gross pathology and histopathological changes in the organs of the cerebrum, cerebellum, spinal cord, and sciatic nerve in bali ducks infected with Avian orthoavulavirus type 1 Tabanan-1/ARP/2017 isolate. The present study used 15 male bali ducks aged 21 days which had been acclimatized for two weeks. Ten ducks were treated for infection (P1) were infected with AOAV-1 Tabanan-1/ARP/2017 isolate at a dose of 0.5 ml/27 HAU intraocularly, and 5 control ducks (P0) were given Phosphate Buffered Saline solution as much as 0.5 ml intraocularly. Then the dead ducks were necropsied for gross pathology examination. The nervous organs were processed and stained for hematoxylin-Eosin, then performed histopathological examination. Gross pathological change was found encephalomalacia in the cerebrum and cerebellum. Histopathological changes were dominated by congestion, perivascular edema, endothelial proliferation, neuropil vacuolization, vasculitis, and gliosis of central nervous system organs. Meanwhile, the histopathological appearance of sciatic nerve is congestion.
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References
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Yusoff K, Tan W. 2001. Newcastle disease virus: Macromolecules and opportunities. Avian Pathol. 30(5): 439-455.
Adi AAAM, Astawa NM, Putra KSA, Hayashi Y, Matsumoto Y. 2010. Isolation and characterization of a pathogenic newcastle disease virus from a natural case in Indonesia. J. Vet. Med. Sci. 72: 313-319.
Adi AAAM, Astawa NN, Wandia IN, Putra IGAA, Winaya IBO, Krisnandika AAK, Wijaya AAGO. 2019b. Karakteristik molekuler virus avian orthoavulavirus 1 genotip vii yang diisolasi dari Tabanan Bali. J. Vet. 20(4): 593-602.
Amarasinghe GK, Ayllón MA, Bào Y, Basler CF, Bavari S, Blasdell KR, Briese T, Brown PA, Bukreyev A, Balkema-Buschmann A. 2019. Taxonomy of the order Mononegavirales: update 2019. Arch. Virol. 164(7): 1967–1980.
Brown C, King DJ, Seal BS. 1999. Pathogenesis of Newcastle disease in chickens experimentally infected with viruses of different virulence. Vet. Pathol. 36: 125-132.
Butt SL, Moura VMBD, Susta L, Miller PJ, Hutcheson JM, Cardenas-Garcia S, Brown CC, West FD, Afonso CL, Stanton JB. 2019. Tropism of Newcastle disease virus strains for chicken neurons, astrocytes, oligodendrocytes, and microglia. BMC Vet. Res. 15: 317.
Dai Y, Cheng X, Liu M, Shen X, Li J, Yu S, Zou J, Ding C. 2014. Experimental infection of duck origin virulent Newcastle disease virus strain in duck. BMC Vet. Res. 10: 164.
Dai Y, Liu M, Cheng X, Shen X, Wei Y, Zhou S, Yu S, Ding C. 2013. Infectivity and pathogenicity of newcastle disease virus strains of different avian origin and different virulence for mallard ducklings. Avian Dis. 57: 8-14.
Ecco R, Susta L, Afonso CL, Miller PJ, Brown C. 2011. Neurological lesions in chickens experimentally infected with virulent Newcastle disease virus isolates. Avian Pathol. 40(2): 145-152.
Jones TC, Hunt RD, King NW. 1997. Rabies. USA. William & Wilkins.
Kattenbelt JA, Stevens MP, Gould AR. 2006. Sequence variation in the newcastle disease virus genome. Virus Res. 116: 168-184.
Kencana GAY, Kardena IM, Mahardika IGNK. 2012. Peneguhan diagnosis penyakit newcastle disease lapang pada ayam buras di Bali menggunakan teknik RT-PCR. J. Ked. Hewan. 6(1): 28-31.
Kencana GAY. 2012. Penyakit virus unggas. Denpasar. Udayana University Press. Pp. 34-52.
Kiernan JA. 2015. Histological and histochemica methods: theory and practice. 5th ed. United Kingdom. Scion Publishing. Pp. 330-334.
Kingston DJ, Dharsana R, Chavez ER. 1978. Isolation of mesogenic Newcastle disease virus from an acute disease in Indonesian ducks. Trop. Anim. Health Prod. 10: 161-164.
Mohammed FF, Mousa MR, Khalefa HS, El-Deeb AH, Ahmed KA. 2019. New insights on neuropathological lesions progression with special emphasis on residence of velogenic newcastle disease viral antigen in the nervous system of experimentally infected broiler chickens. Explor Anim. Med. Res. 9(2): 145-157.
Moura VMBD, Susta L, Cardenas-Garcia S, Stanton JB, Miller PJ, Afonso CL, Brown CC. 2015. Neuropathogenic capacity of lentogenic, mesogenic, and velogenic newcastle disease virus strains in day-old chickens. Vet. Pathol. 53(1): 53–64.
Nishikawa M, Paulillo AC, Nakaghi LSO, Nunes AD, Campioni JM, Doretto JL. 2007. Newcastle disease in white Pekin ducks: response to experimental vaccination and challenge. Braz. J. Poult. Sci. 9: 123–125.
Onapa MO, Christensen H, Mukiibi GM, Bisgaard M. 2006. A preliminary study of the role of ducks in the transmission of Newcastle disease virus to in-contact rural free-range chickens. Trop. Anim. Health Prod. 38: 285–289.
Salbahaga DP, Supartika IKE, Berata IK. 2012. Distribusi lesi negri’s bodies dan peradangan pada otak anjing penderita rabies di Bali. Indon. Med. Vet. 1(3): 352-360.
Susta L, Diego GD, Sean C, Cardenas-Garcia S, Roy SS, Miller PJ, Brown CC, Afonso CL. 2015. Expression of chicken interleukin-2 by a highly virulent strain of Newcastle disease virus leads to decreased systemic viral load but does not significantly affect mortality in chickens. J. Virol. 12: 122.
Wulan OH, Niken Y, Hastari W, Raden W. 2017. Detection of newcastle disease virus by immunohistochemistry on the brains of laying birds with clinical signs torticollis and curled toe paralysis. The Vet. Med. Int. Conf. 2017: 286–295.
Yusoff K, Tan W. 2001. Newcastle disease virus: Macromolecules and opportunities. Avian Pathol. 30(5): 439-455.
Published
2022-05-12
How to Cite
SARI, Yeni Ratna; ADI, Anak Agung Ayu Mirah; WINAYA, Ida Bagus Oka.
GROSS PATHOLOGY AND HISTOPATHOLOGY DESCRIPTION OF NERVOUS SYSTEM OF BALI DUCK AFTER EXPERIMENTAL INFECTION WITH AVIAN ORTHOAVULAVIRUS 1 TABANAN-1/ARP/2017 ISOLATE.
Buletin Veteriner Udayana, [S.l.], p. 10-19, may 2022.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/86259>. Date accessed: 21 nov. 2024.
doi: https://doi.org/10.24843/bulvet.2023.v01.i01.p02.
Section
Articles
