THE EFFECTS OF CHITOSAN GEL IN WHITE RAT TOTAL LEUKOCYTES ON INCISION WOUND HEALINGS
Abstract
This study aims to determine the effect of chitosan gel on the number of white mouse leukocytes (Rattus norvegicus) on incision wound healing. This study used 10 female white rats that were 2 months old and weighed ± 165 g, that was designed with a Split-plot design using 10 rats and was divided into 2 treatment groups with each treatment group consisting of 5 replications. Treatment I (control) incision wound smeared with gentamicin ointment, treatment II incision wound smeared with chitosan gel 2 times a day for 14 consecutive days. This study used chitosan gel with a 5% concentration. Blood sampling through the lateral tail vein as much as 0.5 ml and observation of the number of leukocytes was carried out on days 0, 3, 7 and 14 after treatment. Examination of the number of leukocytes is done with a hemocytometer and using the Neubauer method. The parameters of this study are the number of leukocytes in the incision wounds healing process in white rats. The research data were analyzed using analysis of variance based on the Split-plot Design with the SPSS program. The number of leukocytes in group I (gentamicin) vs group II (chitosan) on days 0; 3; 7; and 14 was 7.89×104/mm3 vs 7.30×104/mm3; 6.28×104/mm3 vs 10.23×104/mm3; 7.27×104/mm3 vs 10.28×104/mm3; and 7.02×104/mm3 vs 7.52×104/mm3, respectively. From the results of this study it can be concluded that the use of chitosan gel is better than gentamicin ointment in the healing process of incision wounds in white rats. The results of thisstudy can be followed by conducting research on other animals for wound treatment applications.
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References
Aulia A, Candra A. 2015. Pengaruh pemberian seduhan daun kelor (Moringa oleifera lam) terhadap jumlah leukosit tikus putih (Rattus norvegicus). J. Nut. Coll. 4(2): 308-313.
Burgaleta C, Martinez-Beltran J, Bouza E. 1982. Comparative effects of moxalactam and gentamicin on human polymorphonuclear leukocyte functions. Antimicrob. Agens Chemotherapy. 21(5): 718-72.
Cockbill S. 2002. Wounds The Healing Process. The Welsh School of Pharmacy, University College, Cardiff.
Darwin CO. 2016. Gambaran sel darah putih pada respon inflamasi pasca pemasangan implan yang dilapisi platelet rich plasma dan tanpa dilapisi platelet rich plasma. Thesis. Fakultas Kedokteran Gigi Universitas Hasanuddin Makasar, Makassar.
Huttenlocher A, Horwitz AR. 2007. Wound healing with electric potential. N. Engl. J. Med. 356(3): 303-304.
Mackay D, Alan LM. 2003. Nutritional support for wound healing. Alt. Med. Rev. 8(4): 359-377.
Masir O, Manjas M, Putra AE, Agus S. 2012. Pengaruh cairan Cultur Filtrate Fibroblast (CFF) terhadap penyembuhan luka penelitian eksperimental pada Rattus norvegicus galur wistar. J. Kes. Andalas, 1(3): 112-117.
Nur M. 2014. Pengaruh kitosan terhadap jumlah osteoklas dan osteoblas pada tikus galur wistar model menopause. J. Pus. Kes. 4(3): 76-87.
Perdanakusuma DS. 2007. Anatomi Fisiologi Kulit dan Penyembuhan Luka. Airlangga University School of Medicine, Surabaya.
Putri RF, Tasminatun S. 2012. Efektivitas salep kitosan terhadap penyembuhan luka bakar kimia pada Rattus norvegicus. Mut. Med. 12(1): 24-30.
Rafdinal I, Amiruddin, Asmilia N, Zuraidawati, Sayuri A, Zuhrawati, Daud R. 2016. Perbedaan jumlah leukosit setelah transplantasi kulit secara autograft dan isograft pada anjing lokal (Canis lupus familiaris). J. Med. Vet. 4(2): 144-146.
Sukorini U, Dwi KN, Rizki M, Bambang HPJ. 2010. Pemantapan Mutu Internal Laboratorium Klinik. Yogyakarta, Alfamedia.
Ueno H, Mori T, Fujinaga T. 2001. Topical formulations and wound healing applications of chitosan. Adv. Drug Delivery Rev. 52(2): 105-115.
Ueyama Y, Misaki M, Ishihara Y, Matsumura T. 1994. Effects of antibiotics on human polymorphonuclear leukocyte chemotaxisin vitro. British J. Oral Maxillofacial Surg. 32(2): 96-99.
Wardono AP, Pramono BH, Husein RAJ, Tasminatun S. 2012. Pengaruh kitosan secara topikal terhadap penyembuhan luka bakar kimiawi pada kulit Rattus norvegicus. Mut. Med. 12(3): 178-187.
