TOTAL COLIFORM BACTERIA IN PIG FARM WASTE IN BADUNG DISTRICT OF BALI PROVINCE
Abstract
Pig farm waste is residual waste from a pig farming activity such as urine, feces, feed residue and cage washing water. The purpose of this study was to determine the Total Coliform bacteria in pig farm waste in Badung District of Bali province. This research is an observational study, the location of the farm was chosen by purposive sampling. Sampling was carried out in four sub-districts, namely Petang sub-district, Abiansemal sub-district, Mengwi sub-district, and North Kuta sub-district. The volume of pig farm waste samples taken 500 ml of each farm then mixed into one per sub-district. The volume of pig farm waste samples taken 500 ml per farm. In each sub-district, has 3 three farmer waste then mixed into one sample per sub-district. In this study, the Coliform bacterial culture method used the spread method on Eosin Methelyne Blue Agar media. Positive samples are indicated by green metallic and pink colonies. The obtained data were analyze using One Way Anova. The results total Coliform are in Petang sub-district 175x108 CFU/mL, Abiansemal sub-district 142x108 CFU/mL, Megwi sub-district 114x108 CFU/mL and North Kuta sub-district 99x108 CFU/mL. Based on the results One Way Anova, it can be concluded the total number of Coliform bacteria in pig farm waste in Petang sub-district, Abiansemal sub-district, Mengwi sub-district and North Kuta sub-district was not significantly different (P>0,05). Pig farm waste with total Coliform bacteria that exceeds the standard limit needs to be treated first before being distributed to the environment.
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References
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Suardana IW, Putri PJRA, Besung INK. 2016. Isolasi dan identifikasi Escherichia coli O157:H7 pada feses sapi di Kecamatan Petang, Kabupaten Badung-Bali. Bul. Vet. Udayana. 8(1): 30-35.
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Sumadi IK. 2015. Pengaruh suplementasi starbio dalam pakan dengan 40% dedak padi terhadap penampilan babi landrace. Maj. Ilmiah Pet. 18(1): 30-34.
Supadma AAN, dan Arthagama DM. 2008. Uji formulasi kualitas pupuk kompos yang bersumber dari sampah organik dengan penambahan limbah ternak ayam, sapi, babi dan tanaman pahitan. J. Bumi Lestari. 8(2): 113-121.
Utomo S, Wahyuningsih. 2010. Dosis campuran limbah sapi dan limbah babi terhadap produksi gasbio. J. Agri. Sains. 1(1): 7-14
Venglovsky J, Sasakova N, Gregova G, Papajova I, Toth F, Szaboova T. 2018. Devitalisation of pathogens in stored pig slurry and potential risk related to its application to agricultural soil. Environ. Sci. Pollut. Res. 25(22): 21412-21419.
Waluyo T. 2016. Deteksi jumlah bakteri Coliform pada proses pembentukan biogas feses sapi potong reaktor tipe fixed-dome. Students e-J. 5(4): 1-6.
Antara S, Gunam IBW. 2002. Dunia Mikroba (Bahaya Mikrobiologis pada Makanan). Denpasar: Pusat Kajian Keamanan Pangan. Universitas Udayana
Besung INK. 2012. Kejadian kolibasilosis pada anak babi. Maj. Ilmiah Peternakan. 13(1): 1-12.
BPS Provinsi Bali. 2020. Keadaan Meteorologi dan Geofisika Menurut Kabupaten/Kota di Provinsi Bali.
Budaarsa K. 2014. Potensi ternak babi dalam menyumbangkan danging di Bali. Proc. Seminar Nasional Ternak Babi. Denpasar, 5 Agustus 2014.
Cappuccino JG, Sherman N. 2002. Microbiology a Laboratory Manual. The Benjamin/Cummings. Publishing Company, Inc. Menlo Park. California.
Conboy MJ, Goss MJ. 2001. Identification of an assemblage of indicator organisms to assess timing and source of bacterial contamination in groundwater. Water, Air, and Soil Pollut. 129(1): 101-118.
Cools D, Merckx R, Vlassak K, Verhaegen J. 2001. Survival of E. coli and Enterococcus spp. derived from pig slurry in soils of different texture. Appl. Soil Ecol. 17(1): 53-62.
Effendi H. 2007. Telaah kualitas air bagi pengelola sumber daya dalam sudarmadji. hidrologi dan klimatologi kesehatan. bahan ajar. Skripsi. Jurusan Kesehatan Lingkungan. Universitas Gajah Mada. Yogyakarta.
Entry JA, Phillips I, Stratton H, Sojka RE. 2003. Polyacrylamide+ Al2 (SO4)3 and polyacrylamide+ CaO remove Coliform Bacteria and nutrients from swine wastewater. Environmen. Pollut. 121(3): 453-462.
Fairbrother JM, Nadeau É. 2019. Colibacillosis. Dis. Swine. 2019: 807-834.
Fardiaz S. 1992. Mikrobiologi Pangan 1. PT Gramedia Pustaka Utama, Jakarta.
Grudziński M, Pietruszka A, Sawicki W. 2015. Anaerobic digestion in sanitization of pig slurry and biomass in agricultural biogas plant. J. Microbiol. Biotechonol. Food Sci. 4(6): 524-526.
Hutajulu YME, Asnita R, Hartono, Silalahi MI, Pane PY. 2021. Analisis pencemaran air sungai denai akibat pembuangan limbah perternakan babi di lingkungan jermal baru. Jumantik. 6(1): 57-64.
Karnchanawong S. Koottatep S. 1993. Monitoring and evaluation of shallow well water quality near a waste disposal site. Enviromen. Int. 19: 579-587.
Khotimah S. 2013. Kepadatan bakteri Coliform di sungai kapuas Kota Pontianak. Proc. Semirata 2013.
Kowalski Z, Makara A, Fijorek K. 2013. Changes in the properties of pig manure slurry. Acta. Biochim. Polonica. 60(4): 845-850.
Lestari NPI, Permatasari AAAP. 2018. Pengaruh suhu dan waktu simpan terhadap populasi total bakteri, Coliform dan Escherichia coli pada ikan nila (Oreochromis niloticus). J. Med. Sains. 2(2): 96-103.
Marszałek M, Kowalski Z, Makara A. 2014. Physicochemical and microbiological characteristics of pig slurry. Tech. Trans. Chem. 18: 81-91.
Mieszkin S, Furet JP, Corthier G, Gourmelon M. 2009. Estimation of pig fecal contamination in a river catchment by real-time PCR using two pig-specific bacteroidales 16S Rrna genetic markers. Appl. Environ. Microbiol. 75(10): 3045-3054.
Monroy F, Aira M, Domínguez J. 2009. Reduction of total Coliform numbers during vermicomposting is caused by short-term direct effects of earthworms on microorganisms and depends on the dose of application of pig slurry. Sci. Total Environ. 407(20): 5411-5416.
Park JH, Kim YJ, Seo KH. 2018. Spread of multidrug-resistant Escherichia Coli harboring integron via swine farm waste water treatment plant. Ecotoxicol. Environ. Safety.149: 36-42.
Paruch AM, Mæhlum T. 2012. Specific features of Escherichia coli that distinguish it from Coliform and thermotolerant Coliform bacteria and define it as the most accurate indicator of faecal contamination in the environment. Ecological Indicators. 23: 140-142.
Rudiyansyah A, Wahyuningsih NE, Kusumanti E. 2015. Pengaruh Suhu, kelembaban, dan sanitasi terhadap keberadaan bakteri Eschericia Coli dan Salmonella di kandang ayam pada peternakan ayam broiler Kelurahan Karanggeneng Kota Semarang. J. Kes. Mas. 3(2): 196-201
Sabaaturohma CL, Gelgel KTP, Suada IK 2020. Jumlah cemaran bakteri Coliform dan non-Coliform pada air di RPU di Denpasar melampaui baku mutu nasional. Indon. Med. Vet. 9(1): 139-147.
Saputri TS, Makhfud E. 2020. The density of the bacteria Coliform as an indicator of biological pollution in the sepuluh coastal waters of The Bangkalan Regency. Juvenil: J. Ilmiah Kelautan dan Perikanan. 1(2): 243-249.
Sihombing DTH. 1997. Ilmu Ternak Babi. Fakultas Peternakan IPB, Bogor.
Sihombing DTH. 2006. Petunjuk Praktis Beternak Babi. Fakultas Peternakan IPB.
Suardana IW, Putri PJRA, Besung INK. 2016. Isolasi dan identifikasi Escherichia coli O157:H7 pada feses sapi di Kecamatan Petang, Kabupaten Badung-Bali. Bul. Vet. Udayana. 8(1): 30-35.
Suarjana IGK. 2009. Kualitas air minum ternak ayam petelur di Desa Piling Kecamatan Penebel Kabupaten Tabanan ditinjau dari jumlah bakteri Coliform. Bul. Vet. Udayana. 1(2): 55-60.
Sumadi IK. 2015. Pengaruh suplementasi starbio dalam pakan dengan 40% dedak padi terhadap penampilan babi landrace. Maj. Ilmiah Pet. 18(1): 30-34.
Supadma AAN, dan Arthagama DM. 2008. Uji formulasi kualitas pupuk kompos yang bersumber dari sampah organik dengan penambahan limbah ternak ayam, sapi, babi dan tanaman pahitan. J. Bumi Lestari. 8(2): 113-121.
Utomo S, Wahyuningsih. 2010. Dosis campuran limbah sapi dan limbah babi terhadap produksi gasbio. J. Agri. Sains. 1(1): 7-14
Venglovsky J, Sasakova N, Gregova G, Papajova I, Toth F, Szaboova T. 2018. Devitalisation of pathogens in stored pig slurry and potential risk related to its application to agricultural soil. Environ. Sci. Pollut. Res. 25(22): 21412-21419.
Waluyo T. 2016. Deteksi jumlah bakteri Coliform pada proses pembentukan biogas feses sapi potong reaktor tipe fixed-dome. Students e-J. 5(4): 1-6.
Published
2022-05-12
How to Cite
ALEGRIA, Cesarina Pascalia dos Santos; SUADA, I Ketut; AGUSTINA, Kadek Karang.
TOTAL COLIFORM BACTERIA IN PIG FARM WASTE IN BADUNG DISTRICT OF BALI PROVINCE.
Buletin Veteriner Udayana, [S.l.], p. 668-675, may 2022.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/79949>. Date accessed: 19 apr. 2024.
doi: https://doi.org/10.24843/bulvet.2022.v14.i06.p09.
Section
Articles
