TOTAL PROTEIN LEVEL OF BALI CATTLE POST TRANSPORTATION IN SLAUGHTERHOUSES PESANGGARAN DENPASAR
Abstract
This study aims to determine the effect of transportation on changes in total protein levels in Bali cattle after a trip to the Pesanggaran Slaughterhouse (RPH) Denpasar. Transportation can lead to stress and dehydration. To diagnose the occurrence of stress can be done by biochemical tests. The biochemical test carried out in this study was to measure the levels of total protein in the blood. The object used is Bali cattle that have not been rested after transportation as many as 20 heads at the Denpasar Pesanggaran Slaughterhouse. Blood was taken through the auricular vein and put into a vacutainer tube containing a gel separator to separate serum from blood. Blood serum samples were taken to the Bali Provincial Health Laboratory for immediate examination of total protein levels using the Photometer 5010V5+ machine. The total protein content of the examined samples had the lowest level of 5.8 g/dL and the highest 8.5 g/dL with an average of 7.5 g/dL. From the results of the study, the total protein content of Bali cattle after transportation was considered normal, this indicated that there was no effect of transportation on the total protein content.
Downloads
References
Anton A, Kasip LM, Wirapribadi L, Depamede SN, Asih ARS. 2016. Perubahan status fisiologis dan bobot badan sapi bali bibit yang diantarpulaukan dari Pulau Lombok ke Kalimantan Barat. J. Ilmu Teknol. Peternakan Indon. 2(1): 86-95.
Aradom S. 2013. Animal transport and welfare with special emphasis on transport time and vibration. Doctoral Thesis. Uppsala. Swedish University of Agricultural Sciences, Uppsala.
Besung INK, Watiniasih NL, Mahardika IGNK, Agustina KK, Suwiti NK. 2019. Mineral levels of Bali cattle (Bos javanicus) from different types of land in Bali, Nusa Penida, and Sumbawa Islands (Indonesia). Biodiversitas. 20(10): 2931-2936.
Bulitta FS, Aradom S, Gebresenbet G. 2015. Effect of transport time of up to 12 hours on welfare of cows and bulls. J. Serv. Sci. Manag. 8: 161-182.
Dewi AKS, Mahardika IG, Dharmawan NS. 2018. Total eritrosit, kadar hemoglobin, nilai hematokrit sapi bali lepas sapih diberi pakan kandungan protein dan energi berbeda. Indon. Med. Vet. 7(4): 413-421.
Frandson RD. 1992. Anatomi dan fisiologi ternak edisi keempat. Gadjah Mada University Press. Yogyakarta.
Gallo CB, Huertas SM. 2016. Main animal welfare problems in ruminant livestock during preslaughter operations: a South American view. Animal. 10(2): 357-364.
Genswein KS, Faucitano L, Dadgar S, Shand P, González LA, Crowe TG. 2012. Road transport of cattle, swine and poultry in North America and its impact on animal welfare, carcass and meat quality: a Review. J. Meat Sci. 92(3): 227-243.
Girindra A. 1988. Biokimia I. Jakarta: Gramedia.
Handiwirawan E, Subandryo. 2004. Potensi keragaman sumber daya genetik Sapi bali. Wartazoa. 14(3): 107-115.
Hartung J, Marahrens M, Holleben KV. 2003. Recommendations for future development in cattle transport in Europe. Dtsch. Tierärztl. Wschr. 110(3): 128-130.
Irfan IZ, Izfandiari A, Choliq C. 2014. Profil protein total, albumin, globulin dan rasio albumin globulin sapi pejantan. J. Ilmu Ternak Vet. 19(2): 123-129.
Issaq HJ, Conrads TP, Janini GM, Veenstra TD. 2002. Methods for fractionation, separation and profiling of proteins and peptides. Electrophoresis. 23: 3048–3061.
Kaneko JJ. 1997. Serum proteins and the dysproteinemias. Di dalam Kaneko JJ, JW. Harvey, ML Bruss, editor. Clinical Biochemistry of Domestic Animals. Edisi 5. Academic press. London.
Kaslow JE. 2010. Analysis of serum protein. Santa Ana: 720 North Tustin Avenue Suite 104, CA.
Lemeshow S. 1997. Besar sampel dalam penelitian kesehatan. Yogyakarta: Gadjah Mada University Press.
Pieper R, Gatlin CL, Makusky AJ, Russo PS, Schatz CR, Miller SS, Su Q, McGrath AM, Estock MA, Parmar PP, Zhao M, Huang ST, Zhou J, Wang F, Esquer-Blasco R, Anderson NL, Taylor J, Steiner S. 2003. The human serum proteome: dis-play of nearly 3700 chromatographically separated protein spots on two-dimensional electrophoresis gels and identification of 325 distinct proteins. Proteomics. 3: 1345–1364.
Schwartzkopf-Genswein K, Grandin T. 2014. Cattle transport by road. CAB International.
Senja NO, Widyastuti SK, dan Erawan IGMK. 2020. Kadar protein total serum sapi bali betina di sentra pembibitan sapi bali Desa Sobangan, Badung. Indon. Med. Vet. 9(4): 502-511.
Soares FS, Dryden GM. 2011. A body condition scoring system for Bali cattle. Asian-Australasian J. Anim. Sci. 24(11): 1587–1594.
Swanson JC, Morrow-Tesch J. 2001. Cattle transport: Historical, research, and future perspectives. J. Anim. Sci. 79: E102–E109.
Oka IGL, Suyadnya IP, Putra S, Suarna IM, Suparta N, Saka I K, Suwiti NK, Antara IM, Puja IN, Sukanata IW, Oka AA, Mudita IM. 2012. Sapi bali sumberdaya genetik asli Indonesia. Denpasar, Udayana University Press. 351 Hlm.
