BLOOD RESULTS IN PIGS INFECTED WITH STREPTOCOCCUS SUIS INTRANASALLY AND INTRAVENOUSLY
Abstract
Research has been carried out to elucidate clinical, as well as differences in blood profiles in pigs infected with Streptococcus suis isolated from Bali by intravenous and intranasal infection routes. S.suis isolate of PPB5 were inoculated intranasally and intravenously to three pigs respectively. The pigs used in this study were 8 weeks old. In intranasal infection before inoculation, 1% acetic acid was first applied to the nasal cavity, then S. suis inoculum was sprayed (2 x 106). In intravenous infection, inoculum fluid is injected into the auricular vein. Clinical changes in pigs were observed until day 15. Blood samples were taken before being inoculated with bacteria, 3rd day after inoculation, and before being euthanized. On the 3rd day after inoculation, the number of eosinophils was significantly different in the two treatments, while the other blood profiles were not significantly different. All blood indicators in the two treatments were not significantly different before the animals were euthanized on day 15. The increase number of erythrocytes and monocytosis was found in intranasal infections at that day. Hypohaemoglobinemia and decreased PCV are seen in both intranasal and intravenous infections. Further research is needed with a greater number of replications. The S. suis vaccine needs to be developed immediately to reduce the threat to public health and economic losses for farmers.
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Boschert K, Flecknell PA, Fosse RT, Framstad T, Ganter M, Sjostrand U, Stevens J, Thurmon J. 1996. Ketamine and its use in the Pig. Lab. Anim. 30: 209-219.
Destriana Y. 2011. Uji efektivitas lidah buaya (Aloe vera) melalui pakan komersil sebagai imunostimulan pada benih lele dumbo (Clarias gariepinus) terhadap infeksi bakteri Aeromonas hydrophila. Program Studi Perikanan. Fakultas Perikanan dan Ilmu Kelautan. Universitas Padjadjaran. Bandung. Pp. 78.
Gottschalk M, Segura M, Xu J. 2007. Streptococcus suis infections in humans: the Chinese experience and the situation in North America. Anim Health Res Rev. 8: 29–45.
Gottschalk M, Segura M. 2000. The pathogenesis of meningitis caused by Streptococcus suis: the unresolved questions. Vet. Microbiol. 76: 259-272.
Gottschalk M, Xu J, Lecours MP, Grenier D, Fittipaldi N, Segura M. 2010. Streptococcus suis infections in humans: what is the prognosis for western countries? (Part II). Clin. Microbiol. Newslett. 32: 97–102.
Halbur P, Thanawongnuwech R, Brown G, Kinyon J, Roth J, Thacker E, Thacker B. 2000. Efficacy of antimicrobial treatments and vaccination regimens for control of porcie reproductive and respiratory syndrome virus and Streptococcus suis coinfection of nursery pigs. J. Clin. Microbiol. 38(3): 1156-1160.
Hardi EH. 2011. Kandidat vaksin potensial Streptococcus agalactiae untuk pencegahan penyakit streptococcosis pada ikan nila (Oreochromis niloticus). Disertasi. Sekolah Pascasarjana. Institut Pertanian Bogor. Bogor. Pp. 162.
Hedrick RP, Gilad O, Yun SC, Spangerberg, JV, Marty GD, Nordhausen RW, Kebus MJ, Bercovier H, Eldar A. 2000. A herpes virus associated with mass mortality of juvenile and adult koi, a stain of common carp. American fisheries society. J. Aquatic Anim. Health. 12: 44-57.
Kim D, Han K, Oh Y, Kim CH, Kang I, Lee J, Gottschalk M, Chae C. 2010. Distribution of capsular serotypes and virulence markers of Streptococcus suis isolated from pigs with polyserositis in Korea. Can. J. Vet. Res. 74: 314-316.
Salasia SIO, Haryanto BD, Suarjana IGK, Purwanto A, Haryadi M. 2002. Potensi Zoonotik Streptococcus equi subsp. zooepidemicus: Karakterisasi isolat asal manusia, kera dan babi di Bali. J. Sain Vet. 20(1): 48-52.
Salasia SIO, Nugroho W, Ruff N. 2011. Streptococcus suis infection of pigs in Papua. Humboldt Kolleg-ICONS 2011. Malang.
Samsuri, Utama IH. 2001. Kepekaan Streptococcus equi Subsp. Zooepidemicus terhadap antimikroba kanamisin, streptomisn, cetriakson dan danofloksasin. J. Vet. 2(4): 111-115.
Santika, A, Ciptoroso, Zainun, Z, Sumarjo, Suroso. 2009. Peningkatan daya tahan tubuh ikan mas terhadap infeksi koi herpes virus (khv) melalui teknik vaksinasi (uji lapang). Laporan Tinjauan Hasil BBPBAT. Sukabumi. Pp. 134-148.
Sapulete IM. 2015. Kajian terhadap kadar imunoglobulin A (IgA) serum yang diinduksi olahraga pada pagi hari. J. e-Biomedik (eBm). 3(2): 562-565.
Sheehan B, Labrie L, Lee YS, Wong FS, Chan J, Wendover N, Grisez L. 2009. Streptococcosis in tilapia, vaccination effective against, main strep species. Glob. Aquaculture Alliance. Pp. 72-74.
Tizard IR. 1988. Pengantar Imunologi Veteriner. Ed. 2. Diterjemahkan oleh Partodirejo, M dan Hardjosworo, S. Penerbit Universitas Airlangga. Surabaya. Pp. 497.
Tribun Bali. 2017. Breaking news: Dua warga badung bali positif meningitis suis, Terkena Dari Hidangan Babi!. Tribun Bali.htm. Tanggal Akses 11 Januari 2019
Wertheim HF, Nghia HD, Taylor W, Schultsz C. 2009. Streptococcus suis: an emerging human pathogen. Clin. Infect. Dis. 48: 617–625.
Wisselink HJ, Smith HE, Stockhofe-Zurwieden N, Peperkamp K, Vecht U. 2000. Distribution of capsular types and production of muramidase released protein (MRP) and extracellular factor (EF) of Streptococcus suis strains isolated from diseased pigs in seven European countries. Vet. Microbiol. 74: 237-248.