THE SECONDARY ANTIBODY TITER AGAINST STREPTOCOCCUS SUIS VACCINE WITH DIFFERENT ADJUVANTS IN RAT

  • Sumanna Rumapea Mahasiswa Program Profesi Dokter Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia
  • I Gusti Ngurah Kade Mahardika Laboratorium Biomedik dan Biologi Molekuler Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. Raya Sesetan, Gg. Markisa No.6, Denpasar, Bali, Indonesia
  • I Nengah Kerta Besung Laboratorium Mikrobiologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB Sudirman, Denpasar, Bali Indonesia

Abstract

Streptococcus suis is a pathogenic bacteria which can cause an infection in animals nor humans. This study aimed to determine the difference in secondary antibody from the administration of S. suis vaccine with different adjuvants. This research is experimental with complete randomized design. Total of 15 rats was divided into 3 groups. Each groups was given S. suis vaccine with adjuvant  MontanideTM ISA 78; MontanideTM ISA 201; MontanideTM Gel 01 by IM. The serum were taken from sinus orbitalis on the week 0 (pravacination), 1st week, 2nd week and 3rd week after booster. The secondary antibody titer were measured by indirect ELISA method. Data were analyzed using analysis of variance, then continued by LSD test. The result showed that S. suis vaccine with different  adjuvants had a significant effect (P<0,05). S. S. suis vaccine with MontanideTM ISA 78 (w/o) produced the highest secondary antibody (OD = 0,331), then S. suis vaccine with MontanideTM Gel 01 (OD = 0,256). The lowest titer antibody was produced by S. suis vaccine with  MontanideTM ISA 201 (w/o/w) adjuvant (OD = 0,208). From the results of this study, it can be concluded that the S.suis vaccine formulation with MontanideTM ISA 78 (w/o) is the best vaccine formulation in production of secondary antibody.

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References

Alsaid SA, Abdul MDM, Atwa MH, Soliman SM. 2020. Evaluation of the cellular humoral immune respone of sheep vaccinated with inactivated rift valley fever vaccine adjuvanted with montanideTM Gel 01. J. Appl. Sci. 5(1): 22-34.
Aucouturier, J., L. Dupuis, V. Ganne. 2001. adjuvants designed for veterinary and human vaccines. Vaccine. 19(17-19): 2666–2672.
Bouguyon. E., Elodie G, Alexander S, Pauline M, Stepan R, Oleg G, Sebastian D, Nicolas B, dan Juliette B A. 2015. A new adjuvant combined with inactivated influenza enhances specific CD8 T cell respone in mice and decreases symptoms in swine upon challenge. Viral Immunol. 28(9): 1-9.
Burakova Y, Rachel M, Scott M, John RS, Jishu S. 2018. Adjuvants for animals. Viral Immunol. 31(1): 11-22.
Deville S, Juliette BA, Francois B, Vladimir B, Laurent D. 2012. Efficacy of intranasal and spray delivery of adjuvanted live vaccine against nfectious bronchitis virus in experimentally infected poultry. Proc. Vaccinol. 6: 85-92.
Gottschalk M, Segura M. 2000. The pathogenesis of the menigitis caused by Streptococcus suis: the unresolved question. J. Vet. Microbiol. 76: 259-272.
Hartati Y. 2005. Respon kekebalan vaksin influenza pada ayam indukan pedaging strain hubbard. Skripsi. Bogor: Institut Pertanian Bogor.
Hadie W, Angela ML, Sularto, Evi T. 2010. Imunitas maternal terhadap aeromonas hydrophila: pengaruhnya terhadap fekunditas dan daya tetas ikan patin siam (Pangasionodon hypophthalmus). J. Riset Akuakultur. 8: 229-235.
Haizhou Z, Zou J, Dai J, Gao Z. 2012. Water in oil emulsion system and preparation process thereof. J. Eur. Patent Office. 12(82): 1-5.
Kencana GAY, Suartha IN, Paramita NMAS, Handayani AN. 2016. Vaksin kombinasi newcastle disease dengan avian influenza memicu imunitas protektif pada ayam petelur terhadap penyakit tetelo dan flu burung. J. Vet. 17(2): 257-264.
Krasucka DM, Katarzyna K, Aneta DW. Wojciech AC. 2014. Determination of the Viscosity and Density of Veterinary Vaccines. Acta Poloniae Pharm.-Drug Res. 71(6): 1090-1094.
Kurnianto AB, Kencana GAY, Astawa INM. 2017. Respon antibodi sekunder terhadap penyakit tetelo pada ayam peterlur pascavaksinasi ulangan dengan vaksin tetelo aktif. J. Vet. 17(3): 331-336.
Malole. 2005. Bahan Teori dan Praktikum Apresiasi Teknik Virologi dan PCR Penyakit Hewan Aquatik. Balai Besar Karantina Ikan Soekarno-Hatta. Jakarta.
Rajput Z, Iqbal HUS, Xiao C, Ariko AG. 2007. Adjuvant Effects of Saponins on Animal Immune Responses. J. Zhejiang Univ. Sci. B 8(3): 153-161.
Santoso K, Ulfatin KH, Dordia AR, Sri M, Muhammad YR, Denny WH, Abdul Z, Ardilasunu W, Arifin BN, Usamah A, Agus W, Ridi A, Ronald T, Edi S. 2021. Perbandingan deteksi titer antibodi pascavaksinasi rabies berbasis kolorimetri menggunakan ELISA reader dan kamera telepon genggam. J. Vet. 22(1): 79-85.
Slipranata M, Lestari FB, Sandi NA, Salasia SIO. 2016. Potensial ekstrak daun sage (Salvia offcinalis.L) sebagai Anti Streptococcus suis penyebab zoonotik meningitis. J. Sain Vet. 34(2): 198-202.
Suartha IN, Wibawan IWT, Putra IGNN, Dewi NMRK, Mahardika IGNK. 2011. Pemilihan adjuvant pada vaksin avian influenza. J. Ked. Hewan 5(2): 49-52.
Suwartini NK, Kencana GAY, Suartha IN. 2018. Respon imun ayam petelur pascavaksinasi newcastle disease pada peternakan komersial di Desa Denbantas, Kecamatan Tabanan. Indon. Med. Vet. 7(3): 185-193.
Vialle R, Dupuis L, Deville S, Betrand F, Gaucheron J, Aucouturier J. 2010. Microgel particulate adjuvant: characterisation and mechanism of action. Proc. Vaccinol. 2(1):12-16.
Vogel FR. 2000. Improving vaccine performance with adjuvants. Clin. Infect. Dis. 30: S266–S270.
Wisselink HJ, Smith HE, Stockhofe ZN, Peperkamp K, Vecht U. 2000. Distribution of capsular types and production muramidase released protein (MRP) and extracellular factor (EF) of Streptococcus suis strains isolated from diseased pigs in seven european countries. Vet. Microbiol. 74: 237-248.
Published
2022-01-30
How to Cite
RUMAPEA, Sumanna; MAHARDIKA, I Gusti Ngurah Kade; BESUNG, I Nengah Kerta. THE SECONDARY ANTIBODY TITER AGAINST STREPTOCOCCUS SUIS VACCINE WITH DIFFERENT ADJUVANTS IN RAT. Buletin Veteriner Udayana, [S.l.], p. 676-684, jan. 2022. ISSN 2477-2712. Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/75229>. Date accessed: 28 mar. 2024. doi: https://doi.org/10.24843/bulvet.2022.v14.i06.p10.
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Articles