THE SECONDARY ANTIBODY TITER AGAINST STREPTOCOCCUS SUIS VACCINE WITH DIFFERENT ADJUVANTS IN RAT
Abstract
Streptococcus suis is a pathogenic bacteria which can cause an infection in animals nor humans. This study aimed to determine the difference in secondary antibody from the administration of S. suis vaccine with different adjuvants. This research is experimental with complete randomized design. Total of 15 rats was divided into 3 groups. Each groups was given S. suis vaccine with adjuvant MontanideTM ISA 78; MontanideTM ISA 201; MontanideTM Gel 01 by IM. The serum were taken from sinus orbitalis on the week 0 (pravacination), 1st week, 2nd week and 3rd week after booster. The secondary antibody titer were measured by indirect ELISA method. Data were analyzed using analysis of variance, then continued by LSD test. The result showed that S. suis vaccine with different adjuvants had a significant effect (P<0,05). S. S. suis vaccine with MontanideTM ISA 78 (w/o) produced the highest secondary antibody (OD = 0,331), then S. suis vaccine with MontanideTM Gel 01 (OD = 0,256). The lowest titer antibody was produced by S. suis vaccine with MontanideTM ISA 201 (w/o/w) adjuvant (OD = 0,208). From the results of this study, it can be concluded that the S.suis vaccine formulation with MontanideTM ISA 78 (w/o) is the best vaccine formulation in production of secondary antibody.
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References
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