PRIMARY ANTIBODY TITERS AGAINST STREPTOCOCCUS SUIS IN WHITE RATS VACCINATED WTH DIFFERENT ADJUVANTS
Abstract
Streptococcus suis is a gram-positive bacterium causing various diseases in pigs and a zoonosis pathogen. Therefore, an effective vaccine for S. suis is crucial to reduce the economic loss due to this pathogen. Most of the inactivated vaccines required additional substances to help increase the vaccine’s efficacy in inducing antibody titers using adjuvant. The study aimed to know the difference of primary antibody titer of inactivated S. suis vaccine with different adjuvant addition. The adjuvant employed was water in oil (w/o) Montanide ISA 78 VG, water in oil in water (w/o/w) Montanide ISA 201 VG, and gel Montanide 01 PR. The test animals were white rats intramuscularly (IM) vaccinated under a 0.5 ml dosage. Blood sampling was conducted in week-1, -2, and -3 post-vaccination. Then, the blood serum was tested using the ELISA test. Quantitative results of ELISA were observed from the optical density (OD) value measured using the ELISA reader. The results show a significant difference in the OD value with a significance value of 0.001 (P<0.01). The best antibody response was indicated by the highest OD value. Vaccine with adjuvant w/o has the highest OD value i.e. 0,175, when compared with adjuvant gel i.e. 0,143, and adjuvant w/o/w i.e 0,103.
Downloads
Download data is not yet available.
References
Abbas AK, Lichtman AH, Pober JS. 2005. Cellular and molecular immunology. Ed 5. WB Saunders Company. Philadelphia.
Aucouturier J, Dupuis L, Ganne V. 2001. Adjuvants designed for veterinary and human vaccines. Vaccine, 19(17-19), 2666-2672.
Besung INK, Suarjana IGK, Agustina KK, Winaya IBO, Soeharsono H, Suwiti NK, Mahardika IGN. 2019. Isolation and identification of Streptococcus suis from sick pigs in Bali, Indonesia. BMC Res. Notes. 12(1): 1-6.
Bouguyon E, Goncalves E, Shevtsov A, Maisonnasse P, Remyga S, Goryushev O, Deville S, Bertho N, Ben Arous, J. 2015. A new adjuvant combined with inactivated influenza enhances specific CD8 T cell response in mice and decreases symptoms in swine upon challenge. Viral Immunol. 28(9): 524-531.
Esgleas M, Dominguez-Punaro MDLC, Li Y, Harel J, Dubreuil JD, Gottschalk M. 2009. Immunization with SsEno fails to protect mice against challenge with Streptococcus suis serotype 2. FEMS Microbiol. Letters. 294(1): 82-88.
Feng Y, Zhang H, Wu Z, Wang S, Cao M, Hu D, Wang C. 2014. Streptococcus suis infection: an emerging/reemerging challenge of bacterial infectious diseases?. Virulence. 5(4): 477-497.
Hadioetomo RS. 1985. Mikrobiologi Dasar-dasar Praktek. 42-96. Gramedia. Jakarta.
Higgins R, Gottschalk M. 1990. Review article: An update on Streptococcus suis identification. J. Vet. Diagn. Invest. 2: 249-252.
Hsueh KJ, Cheng LT, Lee JW, Chung YC, Chung WB, Chu CY. 2017. Immunization with Streptococcus suis bacterin plus recombinant Sao protein in sows conveys passive immunity to their piglets. BMC Vet. Res. 13(1): 1-9.
Jansen T, Hofmans MP, Theelen MJ, Schijns VE. 2005. Structure–activity relations of water-in-oil vaccine formulations and induced antigen-specific antibody responses. Vaccine. 23(8), 1053-1060.
Klimka A, Michels L, Glowalla E, Tosetti B, Krönke M, Krut O. 2015. Montanide ISA 71 VG is advantageous to Freund's adjuvant in immunization against S. aureus Infection of mice. Scandinav. J. Immunol. 81(5): 291-297.
Li W, Hu X, Liu L, Chen H, Zhou R. 2011. Induction of protective immune response against Streptococcus suis serotype 2 infection by the surface antigen HP0245. FEMS Microbiol. Letters. 316(2): 115-122.
Kresno SB. 2001. Imunologi: Diagnosis dan Prosedur Laboratorium. Ed 4. Jakarta. Penerbit Fakultas Kedokteran, Universitas Indonesia.
Okwumabua O, O'Connor M, Shull E. 2003. A polymerase chain reaction (PCR) assay specific for Streptococcus suis based on the gene encoding the glutamate dehydrogenase. FEMS Microbiol. Letters. 218(1): 79-84.
Parker R, Deville S, Dupuis L, Bertrand F, Aucouturier J. 2009. Adjuvant formulation for veterinary vaccines: Montanide™ Gel safety profile. Proc. Vaccinol. 1(1): 140-147.
Petrovsky N, Aguilar JC. 2004. Vaccine adjuvants: current state and future trends. Immunol. Cell Biol. 82(5): 488-496.
Reed SG, Orr MT, Fox CB. 2013. Key roles of adjuvants in modern vaccines. Nat. Med. 19(12): 1597-1608.
Rajput ZI, Hu S, Xiao C, Arijo AG. 2007. Adjuvant effects of saponins on animal immune responses. J. Zhejiang Univ. Sci. B. 8(3): 153-161.
Segura M. 2015. Streptococcus suis vaccines: candidate antigens and progress. Expert Rev. Vac. 14: 1587–1608.
Segura M, Fittipaldi N, Calzas C, Gottschalk M. 2017. Critical Streptococcus suis virulence factors: are they all really critical?. Trends Microbiol. 25(7): 585–599.
Soeripto. 2002. Pendekatan konsep kesehatan hewan melalui vaksinasi. Bogor. J. Litbang Pertanian. 21(2): 48-55.
Spencer T. 1993. Standardization of serology. Penyakit Hewan. 25(46A): 1-6.
Suartha IN, Wibawan IWT, Putra IGNN, Dewi NMRK, Mahardika IGNK. 2011. Pemilihan adjuvant pada vaksin Avian Influenza. Indonesia J. Vet. Sci. 5(2): 49-52.
Susilawathi NM, Tarini NMA, Fatmawati NND, Mayura PI, Suryapraba AAA, Subrata M, Mahardika GN. 2019. Streptococcus suis–associated meningitis, Bali, Indonesia, 2014–2017. Emerg. Infect. Dis. 25(12): 2235-2242.
Tizard I. 1988. An Introduction to Vetenrinary Immunology. Surabaya. Airlangga University Press.
Verdier F, Barrow PC, Burge J. 2003. Reproductive toxicity testing ofvaccines. Toxicol. 185(3): 213–219.
Vialle R, Dupuis L, Deville S, Bertrand F, Gaucheron J, Aucouturier J. 2010. Microgel particulate adjuvant: characterisation and mechanisms of action. Proc. Vaccinol. 2(1): 12-16.
Wertheim HF, Nghia HD, Taylor W, Schultsz C. 2009. Streptococcus suis: an emerging human pathogen. Clin. Infect. Dis. 48(5): 617-625.
Widyastuti DA. 2013. Profit darah tikus putih wistar pada kondisi subkronis pemberian natrium nitrit. J. Sain Vet. 31(2): 201-215.
Wisselink HJ, Vecht U, Smith HE, Stockhofe‐Zurwieden N. 2001. Protection of pigs against challenge with virulent Streptococcus suis serotype 2 strains by a muramidase‐released protein and extracellular factor vaccine. Vet. Rec. 148(15): 473-477.
Aucouturier J, Dupuis L, Ganne V. 2001. Adjuvants designed for veterinary and human vaccines. Vaccine, 19(17-19), 2666-2672.
Besung INK, Suarjana IGK, Agustina KK, Winaya IBO, Soeharsono H, Suwiti NK, Mahardika IGN. 2019. Isolation and identification of Streptococcus suis from sick pigs in Bali, Indonesia. BMC Res. Notes. 12(1): 1-6.
Bouguyon E, Goncalves E, Shevtsov A, Maisonnasse P, Remyga S, Goryushev O, Deville S, Bertho N, Ben Arous, J. 2015. A new adjuvant combined with inactivated influenza enhances specific CD8 T cell response in mice and decreases symptoms in swine upon challenge. Viral Immunol. 28(9): 524-531.
Esgleas M, Dominguez-Punaro MDLC, Li Y, Harel J, Dubreuil JD, Gottschalk M. 2009. Immunization with SsEno fails to protect mice against challenge with Streptococcus suis serotype 2. FEMS Microbiol. Letters. 294(1): 82-88.
Feng Y, Zhang H, Wu Z, Wang S, Cao M, Hu D, Wang C. 2014. Streptococcus suis infection: an emerging/reemerging challenge of bacterial infectious diseases?. Virulence. 5(4): 477-497.
Hadioetomo RS. 1985. Mikrobiologi Dasar-dasar Praktek. 42-96. Gramedia. Jakarta.
Higgins R, Gottschalk M. 1990. Review article: An update on Streptococcus suis identification. J. Vet. Diagn. Invest. 2: 249-252.
Hsueh KJ, Cheng LT, Lee JW, Chung YC, Chung WB, Chu CY. 2017. Immunization with Streptococcus suis bacterin plus recombinant Sao protein in sows conveys passive immunity to their piglets. BMC Vet. Res. 13(1): 1-9.
Jansen T, Hofmans MP, Theelen MJ, Schijns VE. 2005. Structure–activity relations of water-in-oil vaccine formulations and induced antigen-specific antibody responses. Vaccine. 23(8), 1053-1060.
Klimka A, Michels L, Glowalla E, Tosetti B, Krönke M, Krut O. 2015. Montanide ISA 71 VG is advantageous to Freund's adjuvant in immunization against S. aureus Infection of mice. Scandinav. J. Immunol. 81(5): 291-297.
Li W, Hu X, Liu L, Chen H, Zhou R. 2011. Induction of protective immune response against Streptococcus suis serotype 2 infection by the surface antigen HP0245. FEMS Microbiol. Letters. 316(2): 115-122.
Kresno SB. 2001. Imunologi: Diagnosis dan Prosedur Laboratorium. Ed 4. Jakarta. Penerbit Fakultas Kedokteran, Universitas Indonesia.
Okwumabua O, O'Connor M, Shull E. 2003. A polymerase chain reaction (PCR) assay specific for Streptococcus suis based on the gene encoding the glutamate dehydrogenase. FEMS Microbiol. Letters. 218(1): 79-84.
Parker R, Deville S, Dupuis L, Bertrand F, Aucouturier J. 2009. Adjuvant formulation for veterinary vaccines: Montanide™ Gel safety profile. Proc. Vaccinol. 1(1): 140-147.
Petrovsky N, Aguilar JC. 2004. Vaccine adjuvants: current state and future trends. Immunol. Cell Biol. 82(5): 488-496.
Reed SG, Orr MT, Fox CB. 2013. Key roles of adjuvants in modern vaccines. Nat. Med. 19(12): 1597-1608.
Rajput ZI, Hu S, Xiao C, Arijo AG. 2007. Adjuvant effects of saponins on animal immune responses. J. Zhejiang Univ. Sci. B. 8(3): 153-161.
Segura M. 2015. Streptococcus suis vaccines: candidate antigens and progress. Expert Rev. Vac. 14: 1587–1608.
Segura M, Fittipaldi N, Calzas C, Gottschalk M. 2017. Critical Streptococcus suis virulence factors: are they all really critical?. Trends Microbiol. 25(7): 585–599.
Soeripto. 2002. Pendekatan konsep kesehatan hewan melalui vaksinasi. Bogor. J. Litbang Pertanian. 21(2): 48-55.
Spencer T. 1993. Standardization of serology. Penyakit Hewan. 25(46A): 1-6.
Suartha IN, Wibawan IWT, Putra IGNN, Dewi NMRK, Mahardika IGNK. 2011. Pemilihan adjuvant pada vaksin Avian Influenza. Indonesia J. Vet. Sci. 5(2): 49-52.
Susilawathi NM, Tarini NMA, Fatmawati NND, Mayura PI, Suryapraba AAA, Subrata M, Mahardika GN. 2019. Streptococcus suis–associated meningitis, Bali, Indonesia, 2014–2017. Emerg. Infect. Dis. 25(12): 2235-2242.
Tizard I. 1988. An Introduction to Vetenrinary Immunology. Surabaya. Airlangga University Press.
Verdier F, Barrow PC, Burge J. 2003. Reproductive toxicity testing ofvaccines. Toxicol. 185(3): 213–219.
Vialle R, Dupuis L, Deville S, Bertrand F, Gaucheron J, Aucouturier J. 2010. Microgel particulate adjuvant: characterisation and mechanisms of action. Proc. Vaccinol. 2(1): 12-16.
Wertheim HF, Nghia HD, Taylor W, Schultsz C. 2009. Streptococcus suis: an emerging human pathogen. Clin. Infect. Dis. 48(5): 617-625.
Widyastuti DA. 2013. Profit darah tikus putih wistar pada kondisi subkronis pemberian natrium nitrit. J. Sain Vet. 31(2): 201-215.
Wisselink HJ, Vecht U, Smith HE, Stockhofe‐Zurwieden N. 2001. Protection of pigs against challenge with virulent Streptococcus suis serotype 2 strains by a muramidase‐released protein and extracellular factor vaccine. Vet. Rec. 148(15): 473-477.
Published
2022-01-30
How to Cite
PRAMESTI, Pipit Dwi; MAHARDIKA, I Gusti Ngurah Kade; BESUNG, I Nengah Kerta.
PRIMARY ANTIBODY TITERS AGAINST STREPTOCOCCUS SUIS IN WHITE RATS VACCINATED WTH DIFFERENT ADJUVANTS.
Buletin Veteriner Udayana, [S.l.], p. 363-373, jan. 2022.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/74994>. Date accessed: 13 nov. 2024.
doi: https://doi.org/10.24843/bulvet.2022.v14.i04.p08.
Section
Articles
