SEROPREVALENCE AND PROFILE ANTI-VIRUS OF NEWCASTLE DISEASE POST-VACCINATION ON LOCAL CHICKEN IN BOBONARO DISTRICT TIMOR-LESTE
Abstract
The objective of this study was to determine anti-Newcastle disease virus (NDV) antibody titres in sera samples of pre-vaccinated and post-vaccinated local chickens in the villages of Lahomean, Odomau Raifun and Ritabou, Bobonaro District Timor-Leste. Eighty sera samples of pre-vaccinated and 79 samples of post-vaccinated chickens were collected from village chicken farms bred extensively in all four villages. The sampling techniques adopted were simple random and purposive sampling methods to select subjects based on predetermined criteria. Antibody titers against NDV in serum samples were examined by using hemagglutination test (HA) and hemagglutination inhibition test (HI). The data obtained in this studi were presented descriptively. The results showed that of 80 sera samples collected from pre-vaccinated chickens, 53 (66.25%) sera samples were seronegative with the antibody titers of < 23 (21 – 23) HI units and 27 (33,75%) sera samples were seropositive with antibody titers of 24 – 210 HI units. As for 79 sera samples collected from post-vaccinated chickens, 46 (57%) samples were seronegaitive with the antibody titers of 21 – 23 HI and 33 (43%) sera samples were seropositive with antibody titer of 24 – 211 HI units. The results showed that presence of anti-NDV antibody in prevaccinated chickens was very likely due to the natural infection and vaccination increased the titers and the number of seropositive chickens.
Downloads
References
ADHPI. 2011. Seminar ND Genotip 7B. http://www.adhpi.org/seminar/seminar-nd-genotip-7b/.
Alexander DJ. 1991. Newcastle Disease and Other Paramyxovirus Infections. In: Disease of Poultry 9th Ed. Calnex BW, HJ Barnes, CW Beard, MW Reid, HW Yoder (Eds). Iowa State University Press. Amess. Pp. 496-519.
Alexander DJ. 2000. Newcastle disease and other avian paramyxoviruses. Rev. Sci. Tech. (2): 443-62
Alexander DJ, Jones RC. 2003. Newcastle Disease, Other Avian Paramyxovirus, and Pneumovirus Infections. In: Y.M Saif (Ed.) Disease of poultry, 11th Edition. Iowa State Press. United State. Pp. 63-92.
Akoso BT. 1998. Kesehatan Unggas Panduan Bagi Petugas Teknis, Penyuluhan dan Peternak. Yogyakarta. Kanisius.
Anamu S, Rohi OK. 2005. Studi serologi dengan uji hambatan hemaglutinasi terhadap angsa yang dapat bertindak sebagai pembawa Newcastle Disease di DI Yogyakarta. J. Sain Vet. 1: 8-12.
Bwala DG. 2009. Challenge studies in chickens to evaluate afficasy of commercial newcastle disease vaccines against the strains of the newcastle disease virus prevalent in Sout Africa since 2002. Theses. Faculty of Veterinary Science, University of Pretoria.
Cattoli G, Susta L, Terregino C, Brown C. 2011. Newcastle disease: a review of field recognition and current methods of laboratory detection. JVDI. 23(4): 637-656.
Darmawi F, Wiliana MD, Mahdi A, Faisal J, Zakiah HM. 2015. Deteksi antibodi serum ayam kampung (gallus domesticus) terhadap virus Newcastle Disease di Kota Banda Aceh. JMVet. 0853-1943.
Dortmans JCFM, Koch G, Rottier PJM, Peeters BPH. 2011. Virulence of Newcastle disease virus. Vet. Res. 42: 122.
Halvorson DA. 2002. The Control of H5 orH7 Mildly Pathogenic AI/ND: a role for inactivated vaccine. Avianpathol. Carfax Publishing Ltd. Oxford.
Hewajuli DA, Dharmayanti NLPI. 2015. Peran sistem kekebalan non-spesifik dan spesifik pada unggas terhadap Newcastle Disease. Wartazoa. 25(3): 135-146.
Karaca KD, Swayne, E, Grosenbaugh D, Bublot M, Robles, AS Nordgren. 2005. Immunogenicity of fowlpox virus expressing the avian influenza virus H5 gene in cats. Clin. Diagn. Lab. Immunol. 12(11):1340-1342.
Kencana GAY, Kardena IM, dan Mahardika IGN. 2012. Peneguhan diagnosis penyakit Newcastle disease lapang pada ayam buras di Bali menggunakan teknik RT-PCR J. Ked. Hewan. 6(1): 23-32.
Kencana GAY, Suartha IN, Mesakh PS, Handayan AN, Steffi Ong, Syamsidar, Kusumastuti A. 2015. Respons antibodi terhadap penyakit tetelo pada ayam yang divaksin tetelo dan tetelo-flu burung. J. Vet. 16(2): 283-290.
Kencana GAY, Suartha N, Paramita, NMAS, Handayani AN. 2016. Vaksin kombinasi newcastle disease dengan avian influenza memicu imunitas protektif pada ayam petelur terhadap penyakit tetelo dan flu burung. J. Vet. 17(2): 257-264.
Kencana GAY, Suartha IN, NDR Bonar, TAS Lumban. 2017. Respons imun ayam petelur pascavaksinasi Newcastle Disease. JSV. 35(1): 34-52.
Kim SH, Wanasen N, Palduri A, Xiao S, Collins PL, Samal SK. 2013. Newcastle disease virus fusion protein is the major contributor to protective immunity of genotipe-matched vaccine. Plos One. 8(8): 1-10.
Mayo MA. 2002a. Virus taxonomy-houston. Arc. Virol. 147: 1071-1079.
Mayo MA. 2002b. A summary of taxonomic changes recently approved by ACTV. Arc. Virol. 147: 1655-1663.
Sari PM. 2007. Evaluasi penggunaan bubuk bawang putih (allium sativum) terhadap kandungan lemak darah ayam kampung yang diinfeksi cacing Ascaridia galli. Skripsi. Program Studi Ilmu Nutrisi dan Makanan Ternak Fakultas Peternakan Institut Pertanian Bogor, Bogor.
Syukron MU, IN Suartha, NS Dharmawan. 2013. Serodeteksi penyakit tetelo pada ayam di Timor Leste. Indon. Med. Vet. 2(3):360-368.
Tizard IR. 1987. Pengantar Imunologi Veteriner. Edisi kedua. Universitas Airlangga.
Thrusfield M. 2007. Veterinary Epidemiology. 3rd Edition, Blackwell Science Ltd.Oxford. www.blackwellpublishing.com.
OIE. 2004. Newcastle Disease. Manual of diagnostic tests and vaccines for terrestrial animals, 2: 1–15.
OIE. 2012. Newcastle Disease. 2012. Version adopted by the World Assembly of Delegates, 2: 3-14.
Pramudyati SP. 2009. Petunjuk Teknis Beternak Ayam Buras. Balai Pengkajian Teknologi Pertanian (BPTP), Sumatera Selatan.
