IN-VITRO SCOLICIDAL EFFECTS OF GRAPE AND APPLE VINEGAR ON THE HYDATID CYST PROTSCOLECES
Abstract
At present, surgery remains the preferred treatment for hydatid cyst. Various chemical scolicidal agents have been used for inactivation of protoscoleces during surgery, but most of them are associated with adverse side effects. In this study we investigated the effect of two commercial grape and apple vinegar on the viability of hydatid cyst protoscoleces. The protoscoleces of E. granulosus were aspirated from the infected livers and lungs of sheep slaughtered at Tiaret abattoir. 0.5 mL of two commercial grape and apple vinegar was used for 5, 10, and 15 min in the experiments. 0.1% eosin staining assessed viability of protoscoleces. All tests were carried in triplicate. The mortality rates of protoscoleces were 100% after 5min, 10 min and 15 min of incubation with grape vinegar and apple vinegar. To conclude, the results of the present survey indicated high scolicidal activity of apple vinegar and grape vinegar against protoscoleces of hydatid cyst and can be used in hydatid cyst surgery. However, further studies will be needed to confirm these results by checking this scolicidal activity in an in-vivo model.
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References
Adas G, Arikan S, Kemik O, Oner A, Sahip N, Karatepe O. 2009. Use of albendazole sulfoxide, albendazole sulfone, and combined solutions as scolicidal agents on hydatid cysts (in vitro study). World J. Gastroenterol., 15: 112 116.
Anonymous. US Food and Drug Administration. Acetic Acid - Use in Foods. Available at: http://www.fda.gov/ora/compliance_ref/cpg/cpgfod/cpg562-100.html. Accessed March 9, 2006.
Brunetti E, Kern P, Vuitton DA. 2010. Ex-pert consensus for the diagnosis and treat¬ment of cystic and alveolar echinococcosis in humans. Writing panel for the WHO IWGE. Acta Trop., 114(1): 1–16.
Budke CM, Deplazes P, Torgerson PR. 2006. Global socioeconomic impact of cystic echinococcosis. Emerg. Infect. Dis., 12 : 296–303.
Chen H, Chen T, Giudici P, Chen F. 2016. Vinegar functions on health: constituents, sources, and formation mechanisms. Compr. Rev. Food Sci. Food Saf., 15: 1124e38.
Craig PS, McManus DP, Lightowlers MW, Chabalgoity JA, Garcia HH, Gavidia CM, Gilman RH, Gonzalez AE, Lorca M, Naquira C, Nieto A, Schantz PM. 2007. Prevention and control of cystic echinococcosis. Lancet Infect. Dis., 7(6): 385-394.
Eckert J, Deplazes P. 2004. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin. Microbiol. Rev., 17: 107–115.
Fushimi T, Suruga K, Oshima Y, Fukiharu M, Tsukamoto Y, Goda T. 2006. Dietary acetic reduced serum cholesterol and triacylglycerols in rats feed a cholesterol rich diet. Brit. J. Nutr., 95(5): 916-924.
Hajihossein R, Eslamirad Z, Mosayebi M, Ghasemikhah R, Didehdar M. 2015. In vitro effects of vinegar on protoscolices of hydatid cyst. Asian Pac. J. Trop. Dis., 5(3): 210-213.
Heitor OS, Wilson MAMd-M, Guilherme ARd-S, Jonato P, Brad J S. 2019. Vinegar (acetic acid) intake on glucose metabolism: A narrative review. Clin. Nutr. ESPEN., 32: 1-7.
Houshmand E, Kamalifar H, Elmi H. 2019. In vitro scolicidal effect of ginger (Zingiber officinale Roscoe) ethanolic extract against protoscolices of hydatid cyst. Iran J. Vet. Med., 13(1): 87-99.
Johnston CS, Gaas CA. 2006. Vinegar: medicinal uses and antiglycemic effect. Med. Gen. Med., 8: 61.
Johnston CS, Kim CM, Buller AJ. 2004. Vinegar improves insulin sensitivity to a high carbohydrate meal in subjects with insulin resistance or type 2 diabetes mellitus. Diabetes Care, 27: 281-282.
Junghanss T, da Silva AM, Horton J, Chiodini PL, Brunetti E. 2008. Clinical management of cystic echinococcosis: state of the art, problems, and perspectives. Am. J. Trop. Med. Hyg., 79(3): 301–311.
Kavoosi G, Purfard A M. 2013. Scolicidal effectiveness of essential
oil from Zataria multiflora and Ferula assafoetida: disparity
between phenolic monoterpenes and disulphide compounds. Comp.
Clin. Pathol., 22: 999–1005.
Kishi M, Fukaya M, Tsukamoto Y, Nagasaw T, Takenhana K, Nishizawa N. 1999. Enhancing effect of dietary vinegar on the intestinal absorption of calcium in ovariectomized rats. Biosci. Biotechnol. Biochem., 63: 905-910.
Kohansal MH, Nouriana A, Rahimib MT, Daryanic A, Spotind A, Ahmadpourd E. 2017. Natural products applied against hydatid cyst protoscolices: A review of past to present. Acta Trop., 176: 385–394.
Kondo S, Tayama K, Tsukamoto Y, Ikeda K Yamori Y. 2001. Antihypertensive effects of acetic acid and vinegar on spontaneously hypertensive rats. Biosc. Biotech. Bioch., 65: 2690-2694.
Larki S, Jalali MHR, Goodarzi S. 2017. Scolicidal effects of gallic acid, one of the major compounds of plants, on protoscolices of hydatid cyst. Zahedan J. Res. Med. Sci., 19(5): e9791.
Laurimäe T, Kinkar L, Varcasia A, Dessì G, Sgroi G, D’Alessio N, Veneziano V, Saarma U. 2019. First detection of zoonotic tapeworm Echinococcus granulosus sensu lato genotype G7 in continental Italy. Parasitol. Res., 118(7): 2193-2201.
Mahmoudvand H, Harandi M F, Shakibaie M, Aflatoonian MR, ZiaAli N, Makki MS, Jahanbakhsh S. 2014. Scolicidal effects of biogenic selenium nanoparticles against protoscolices of hydatid cysts. Int. J. Surg., 12: 399-403.
Mahmoudvand H, Kheirandish F, Dezaki ES, Shamsaddini S, Harandi MF. 2016. Chemical composition, efficacy and safety of Pistacia vera (var. Fandoghi) to inactivate protoscoleces during hydatid cyst surgery. Biomed. Pharmacother., 82: 393–398.
Mahmoudvand H, Mirbadie SR, Sadooghian S, Harandi MF, Jahanbakhsh S, Dezaki ES. 2017. Chemical composition and scolicidal activity of Zataria multiflora Boiss essential oil. J. Essent. Oil Res., 29(1): 42-47.
Moazeni M, Nazer A. 2010. In vitro effectiveness of Garlic (Allium sativum) extract on scolices of Hydatid cyst. World J. Surg., 34: 2677-2681.
Moazeni M, Larki S. 2010. In vitro effectiveness of acidic and alkline solutions on scolices of hydatid cyst. Parasitol. Res., 106: 853 856.
Moazeni M, Saharkhiz MJ, Hoseini AA, Alavi AM. 2012. In vitro scolicidal effect of Satureja khuzistanica (Jamzad) essential oil. Asian Pac. J. Trop. Biomed., 2(8): 616-620.
Murooka Y, Yamshita M. 2008. Traditional healthful Industrial fermented products of Japan. J. Ind. Microbiol. Biotechnol., 35: 791-798.
Naseri M, Akbarzadeh A, Spotin A, Akbari NA R, Mahami-Oskouei M, Ahmadpour E. 2016. Scolicidal and apoptotic activities of albendazole sulfoxide and albendazole sulfoxide-loaded PLGA-PEG as a novel nanopolymeric particle against Echinococcus granulosus protoscoleces. Parasitol. Res., 115(12): 4595-4603.
Niazi M, Saki M, Sepahvand M, Jahanbakhsh S, Khatami M, Beyranvand M. 2019. In vitro and ex vivo scolicidal effects of Olea europaea L. to inactivate the protoscoleces during hydatid cyst surgery. Ann. Med. Surg., 42: 7–10.
Rajabi MA. 2009. Fatal reactions and methaemoglobinaemia after silver nitrate irrigation of hydatid cyst. Surg. Pract., 13: 2–7.
Rostami A, Taheri M, Gholizadeh M, Seyyed Tabaei SJ, Raeghi S, Fallahi S. 2016. Scolicidal effect of some herbs on Echinococcus granulosus Protoscoleces: a Systematic Literature Review. Herb. Med. J., 1(1): 1-7.
Sadjjadi SM, Sedaghat F, Hosseini SV, Sarkari B. 2009. Serum antigen and antibody detection in echinococcosis: application in serodiagnosis of human hydatidosis. Korean J. Parasitol., 47(2): 153-7.
Sahim M, Eryilmaz R, Bulbuloglu E. 2004. The effect of scolicidal agents on liver and biliary tree (experimental study). J. Investig. Surg., 17(6): 323.
Shahnazi M, Azadmehr A, Latifi R, Hajiaghaee R, Saraei M, Alipour M. 2016. In vitro protoscolicidal effects of various concentrations of Ziziphora tenuior L. extract at different exposure times. Avicenna J. Phytomed., 6(4): 376–382.
Selles SMA, Kouidri M. 2019. In-vitro Scolicidal Activity of Citrus limon eureka Juice. Agricultura, 1–2 (109-110) : 116-120.
Touhami M, Laroubi A, Elhabazi K, Loubna F, Zrara I, Eljahiri Y, Oussama A, Grases F, Chait A. 2007. Lemon juice has protective activity in rat‘s urolitthiasis model. BMC Urology, 7: 1-10.
Vijayakumar C, Wolf Hall CE. 2002. Evaluation of household sanitizers for reducing levels of Escherichia coli on iceberg lettuce. J. Food Prot., 65: 1646-1650.
Walker M, Rossignol JF, Torgerson P, Hemphill A. 2004. In vitro effects of nitazoxanide on Echinococcus granulosus protoscoleces and metacestodes. J. Antimicrob. Chemother., 54: 609–616.
WHO. 2018. Echinococcosis. Fact Sheet (Updated February 2018). World Health Organization. Available at http://www.who.int/en/ news-room/fact sheets/detail/echinococcosis (last accessed 02.10. 2018)
Zhang W, Li J, McManus DP. 2003. Concepts in immunology and diagnosis of hydatid disease. Clin. Microbiol. Rev., 16: 18–36.
Anonymous. US Food and Drug Administration. Acetic Acid - Use in Foods. Available at: http://www.fda.gov/ora/compliance_ref/cpg/cpgfod/cpg562-100.html. Accessed March 9, 2006.
Brunetti E, Kern P, Vuitton DA. 2010. Ex-pert consensus for the diagnosis and treat¬ment of cystic and alveolar echinococcosis in humans. Writing panel for the WHO IWGE. Acta Trop., 114(1): 1–16.
Budke CM, Deplazes P, Torgerson PR. 2006. Global socioeconomic impact of cystic echinococcosis. Emerg. Infect. Dis., 12 : 296–303.
Chen H, Chen T, Giudici P, Chen F. 2016. Vinegar functions on health: constituents, sources, and formation mechanisms. Compr. Rev. Food Sci. Food Saf., 15: 1124e38.
Craig PS, McManus DP, Lightowlers MW, Chabalgoity JA, Garcia HH, Gavidia CM, Gilman RH, Gonzalez AE, Lorca M, Naquira C, Nieto A, Schantz PM. 2007. Prevention and control of cystic echinococcosis. Lancet Infect. Dis., 7(6): 385-394.
Eckert J, Deplazes P. 2004. Biological, epidemiological, and clinical aspects of echinococcosis, a zoonosis of increasing concern. Clin. Microbiol. Rev., 17: 107–115.
Fushimi T, Suruga K, Oshima Y, Fukiharu M, Tsukamoto Y, Goda T. 2006. Dietary acetic reduced serum cholesterol and triacylglycerols in rats feed a cholesterol rich diet. Brit. J. Nutr., 95(5): 916-924.
Hajihossein R, Eslamirad Z, Mosayebi M, Ghasemikhah R, Didehdar M. 2015. In vitro effects of vinegar on protoscolices of hydatid cyst. Asian Pac. J. Trop. Dis., 5(3): 210-213.
Heitor OS, Wilson MAMd-M, Guilherme ARd-S, Jonato P, Brad J S. 2019. Vinegar (acetic acid) intake on glucose metabolism: A narrative review. Clin. Nutr. ESPEN., 32: 1-7.
Houshmand E, Kamalifar H, Elmi H. 2019. In vitro scolicidal effect of ginger (Zingiber officinale Roscoe) ethanolic extract against protoscolices of hydatid cyst. Iran J. Vet. Med., 13(1): 87-99.
Johnston CS, Gaas CA. 2006. Vinegar: medicinal uses and antiglycemic effect. Med. Gen. Med., 8: 61.
Johnston CS, Kim CM, Buller AJ. 2004. Vinegar improves insulin sensitivity to a high carbohydrate meal in subjects with insulin resistance or type 2 diabetes mellitus. Diabetes Care, 27: 281-282.
Junghanss T, da Silva AM, Horton J, Chiodini PL, Brunetti E. 2008. Clinical management of cystic echinococcosis: state of the art, problems, and perspectives. Am. J. Trop. Med. Hyg., 79(3): 301–311.
Kavoosi G, Purfard A M. 2013. Scolicidal effectiveness of essential
oil from Zataria multiflora and Ferula assafoetida: disparity
between phenolic monoterpenes and disulphide compounds. Comp.
Clin. Pathol., 22: 999–1005.
Kishi M, Fukaya M, Tsukamoto Y, Nagasaw T, Takenhana K, Nishizawa N. 1999. Enhancing effect of dietary vinegar on the intestinal absorption of calcium in ovariectomized rats. Biosci. Biotechnol. Biochem., 63: 905-910.
Kohansal MH, Nouriana A, Rahimib MT, Daryanic A, Spotind A, Ahmadpourd E. 2017. Natural products applied against hydatid cyst protoscolices: A review of past to present. Acta Trop., 176: 385–394.
Kondo S, Tayama K, Tsukamoto Y, Ikeda K Yamori Y. 2001. Antihypertensive effects of acetic acid and vinegar on spontaneously hypertensive rats. Biosc. Biotech. Bioch., 65: 2690-2694.
Larki S, Jalali MHR, Goodarzi S. 2017. Scolicidal effects of gallic acid, one of the major compounds of plants, on protoscolices of hydatid cyst. Zahedan J. Res. Med. Sci., 19(5): e9791.
Laurimäe T, Kinkar L, Varcasia A, Dessì G, Sgroi G, D’Alessio N, Veneziano V, Saarma U. 2019. First detection of zoonotic tapeworm Echinococcus granulosus sensu lato genotype G7 in continental Italy. Parasitol. Res., 118(7): 2193-2201.
Mahmoudvand H, Harandi M F, Shakibaie M, Aflatoonian MR, ZiaAli N, Makki MS, Jahanbakhsh S. 2014. Scolicidal effects of biogenic selenium nanoparticles against protoscolices of hydatid cysts. Int. J. Surg., 12: 399-403.
Mahmoudvand H, Kheirandish F, Dezaki ES, Shamsaddini S, Harandi MF. 2016. Chemical composition, efficacy and safety of Pistacia vera (var. Fandoghi) to inactivate protoscoleces during hydatid cyst surgery. Biomed. Pharmacother., 82: 393–398.
Mahmoudvand H, Mirbadie SR, Sadooghian S, Harandi MF, Jahanbakhsh S, Dezaki ES. 2017. Chemical composition and scolicidal activity of Zataria multiflora Boiss essential oil. J. Essent. Oil Res., 29(1): 42-47.
Moazeni M, Nazer A. 2010. In vitro effectiveness of Garlic (Allium sativum) extract on scolices of Hydatid cyst. World J. Surg., 34: 2677-2681.
Moazeni M, Larki S. 2010. In vitro effectiveness of acidic and alkline solutions on scolices of hydatid cyst. Parasitol. Res., 106: 853 856.
Moazeni M, Saharkhiz MJ, Hoseini AA, Alavi AM. 2012. In vitro scolicidal effect of Satureja khuzistanica (Jamzad) essential oil. Asian Pac. J. Trop. Biomed., 2(8): 616-620.
Murooka Y, Yamshita M. 2008. Traditional healthful Industrial fermented products of Japan. J. Ind. Microbiol. Biotechnol., 35: 791-798.
Naseri M, Akbarzadeh A, Spotin A, Akbari NA R, Mahami-Oskouei M, Ahmadpour E. 2016. Scolicidal and apoptotic activities of albendazole sulfoxide and albendazole sulfoxide-loaded PLGA-PEG as a novel nanopolymeric particle against Echinococcus granulosus protoscoleces. Parasitol. Res., 115(12): 4595-4603.
Niazi M, Saki M, Sepahvand M, Jahanbakhsh S, Khatami M, Beyranvand M. 2019. In vitro and ex vivo scolicidal effects of Olea europaea L. to inactivate the protoscoleces during hydatid cyst surgery. Ann. Med. Surg., 42: 7–10.
Rajabi MA. 2009. Fatal reactions and methaemoglobinaemia after silver nitrate irrigation of hydatid cyst. Surg. Pract., 13: 2–7.
Rostami A, Taheri M, Gholizadeh M, Seyyed Tabaei SJ, Raeghi S, Fallahi S. 2016. Scolicidal effect of some herbs on Echinococcus granulosus Protoscoleces: a Systematic Literature Review. Herb. Med. J., 1(1): 1-7.
Sadjjadi SM, Sedaghat F, Hosseini SV, Sarkari B. 2009. Serum antigen and antibody detection in echinococcosis: application in serodiagnosis of human hydatidosis. Korean J. Parasitol., 47(2): 153-7.
Sahim M, Eryilmaz R, Bulbuloglu E. 2004. The effect of scolicidal agents on liver and biliary tree (experimental study). J. Investig. Surg., 17(6): 323.
Shahnazi M, Azadmehr A, Latifi R, Hajiaghaee R, Saraei M, Alipour M. 2016. In vitro protoscolicidal effects of various concentrations of Ziziphora tenuior L. extract at different exposure times. Avicenna J. Phytomed., 6(4): 376–382.
Selles SMA, Kouidri M. 2019. In-vitro Scolicidal Activity of Citrus limon eureka Juice. Agricultura, 1–2 (109-110) : 116-120.
Touhami M, Laroubi A, Elhabazi K, Loubna F, Zrara I, Eljahiri Y, Oussama A, Grases F, Chait A. 2007. Lemon juice has protective activity in rat‘s urolitthiasis model. BMC Urology, 7: 1-10.
Vijayakumar C, Wolf Hall CE. 2002. Evaluation of household sanitizers for reducing levels of Escherichia coli on iceberg lettuce. J. Food Prot., 65: 1646-1650.
Walker M, Rossignol JF, Torgerson P, Hemphill A. 2004. In vitro effects of nitazoxanide on Echinococcus granulosus protoscoleces and metacestodes. J. Antimicrob. Chemother., 54: 609–616.
WHO. 2018. Echinococcosis. Fact Sheet (Updated February 2018). World Health Organization. Available at http://www.who.int/en/ news-room/fact sheets/detail/echinococcosis (last accessed 02.10. 2018)
Zhang W, Li J, McManus DP. 2003. Concepts in immunology and diagnosis of hydatid disease. Clin. Microbiol. Rev., 16: 18–36.
Published
2020-08-29
How to Cite
MOKHTARIA, Kouidri; AMMAR, Selles Sidi Mohammed.
IN-VITRO SCOLICIDAL EFFECTS OF GRAPE AND APPLE VINEGAR ON THE HYDATID CYST PROTSCOLECES.
Buletin Veteriner Udayana, [S.l.], p. 180-187, aug. 2020.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/60924>. Date accessed: 20 apr. 2024.
doi: https://doi.org/10.24843/bulvet.2020.v12.i02.p13.
Section
Articles
