ASSESSMENT OF THE ACUTE DERMAL TOXICITY POTENTIAL OF NATURAL VETERINARY MEDICINE MINYAK RAJAS IN WISTAR RATS
Abstract
Toxicity testing of a new product or drug that will circulate in the market is commonly done to determine its efficacy and safety. This study aims to assess the potential toxicity of the dermal acute natural veterinary medicine of Minyak Rajas on female Wistar rats. The test method used refers to the Organization for Economic Co-operation and Development protocol test number 402 of 2017. The test results obtained primary irritation index of zero, and the median acute dermal lethal dose >2000 mg/kg. It can be concluded that Minyak Rajas does not cause acute dermal toxicity topically and is safe for usage.
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References
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Salehi B, Zakaria ZA, Gyawali R, Ibrahim SA, Rajkovic J, Shinwari ZK, Khan T, Sharifi-Rad J, Ozleyen A, Turkdonmez E, Valussi M, Tumer TB, Fidalgo LM, Martorell M, Setzer WN. 2019. Piper species: a comprehensive review on their phytochemistry, biological activities and applications. Molecules. 24(7):1364.
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Sasmito WA, Wijayanti AD, Fitriana I, Sari PW. 2015. The acute toxicity test of herbal medicine in mice based on Organization for Economic Co-operation and Development (OECD). J. Sains Vet., 33(2): 234-239.
Shan CY, Iskandar Y. 2018. Studi kandungan kimia dan aktivitas farmakologi tanaman kunyit (Curcuma longa L.). Farmaka, 16(2): 547-554.
Syahidah A, Saad CR, Hassan MD, Rukayadi Y, Norazian MH, Kamarudin MS. 2017. Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract. Pakistan J. Biolog. Sci. 20: 70-81.
UN. 2015. Globally Harmonized System of Classification and Labelling of Chemicals (GHS). Fifth Revised Edition, United Nation, New York and Geneva.
Vaughn AR, Branum A, Sivamani RK. 2016. Effects of turmeric (Curcuma longa) on skin health: a systematic review of the clinical evidence. Phytother. Res. 30(8): 1243‐1264.
Ahlina FN, Nugraheni N, Salsabila IA, Haryanti S, Da'i M, Meiyanto E. 2020. Revealing the reversal effect of galangal (Alpinia galanga L.) extract against oxidative stress in metastatic breast cancer cells and normal fibroblast cells intended as a Co- Chemotherapeutic and anti-ageing agent. Asian Pac. J. Cancer Prev., 21(1): 107‐117.
Ahmad RS, Hussain MB, Sultan MT, Arshad MS, Waheed M, Shariati MA. 2020. Biochemistry, safety, pharmacological activities, and clinical applications of turmeric: a mechanistic review. eCAM, 2020(7656919):14.
Ali A, Akhtar N, Mumtaz AM, Khan MS, Iqbal FM, Zaidi SS. 2013, In vivo skin irritation potential of cream containing Moringa oliefera leaf extract. African J. Pharm. Pharmacol., 7: 289-293.
Amagai Y, Katsuta C, Nomura Y, Oida K, Matsuda K, Jang H, Ahn G, Hamasaki T, Matsuda H, Tanaka A. 2017. Amelioration of atopic-like skin conditions in NC/Tnd mice by topical application with distilled Alpinia intermedia Gagnep extracts. J. Dermatol., 44(11): 1238‐1247.
Anderson SE, Meade BJ. 2014. Potential health effects associated with dermal exposure to occupational chemicals. Environ. Health Insights, (8): 51–62.
Anggraeni N, Syamsunarno MRA, Widyastuti R, Puspitasari IM, Praptama S. 2019. Potential dual effect anti-inflammatory and anti-platelet of cogon grass ethanol extract on diabetic mice a preliminary study. IOP Conf. Series: J. Physics. 1246: 012006.
Baldisserotto A, Buso P, Radice M, Dissette V, Lampronti I, Gambari R, Manfredini S, Vertuani S. 2018. Moringa oleifera leaf extracts as multifunctional ingredients for natural and organic sunscreens and photoprotective preparations. Molecules, 23:664.
Banerjee S, Chattopadhyay P, Ghosh A, Pathak MP, Singh S, Veer V. 2013. Acute dermal irritation, sensitization, and acute toxicity studies of a transdermal patch for prophylaxis against anatoxin-a poisoning. Int. J. Toxicol., 32(4): 308-313.
Berata IK, Winaya IBO, Adi AAAM, Adyana IBW. 2018. Patologi Veteriner Umum. Swasta Nulus. Denpasar.
Gatne MM, Tambe K, Adarsh, Ravikanth K. 2015. Acute dermal irritation study of polyherbal gel mastilep in rabbits. Int. J. Pharm. Sci. Res., 6(8): 3473-3476.
Hakim ZR, Purbarini KA, Tjiptasurasa. 2018. Uji iritasi akut dermal pada hewan uji kelinci albino terhadap sediaan body lotion ekstrak kulit biji pinang (Areca Catechu L.). Farmaka, 18(1): 1-13.
Intahphuak S, Khonsung P, Panthong A. 2010. Anti-inflammatory, analgesic, and antipyretic activities of virgin coconut oil. Pharm. Biol., 48(2): 151-157.
Irsan MA, Manggau E, Pakki, Usmar. 2013. Uji iritasi krim antioksidan ekstrak biji lengkeng (Euphoria longana Stend) pada kulit kelinci (Oryctolagus cuniculus). Majalah Farm. Farmakog., 17(2): 55-56.
Kato E, Kubo M, Okamoto Y, Matsunaga Y, Kyo H, Suzuki N, Uebaba K, Fukuyama Y. 2018. Safety assessment of bangle (Zingiber purpureum Rosc.) rhizome extract: acute and chronic studies in rats and clinical studies in human. ACS Omega., 3(11): 15879-15889.
Kaliyadasa E, Samarasinghe BA. 2019. Genus alpinia, a potential power house of bioactives: a review. Int. J. Pharm. Sci. Res., 10(6): 2644-2653.
Liu RH, Fu LN, Chen LY, Ren G., Chen SS, Chen Z. 2010. Chemical constituents and pharmacology study of Imperata cylindrical rhizomes. J. Tradit. Chin. Med., 22(4): 80-83.
Mao QQ, Xu XY, Cao SY, Gan RY, Corke H, Beta T, Li HB. 2019. Bioactive compounds and bioactivities of ginger (Zingiber officinale Roscoe). Foods, 8(6):185.
Nevin KG, Rajamohan T. 2010. Effect of topical application of virgin coconut oil on skin components and antioxidant status during dermal wound healing in young rats. Skin Pharmacol. Physiol., 23(6): 290-297.
NRC. 2011. Guide for the Care and Use of Laboratory Animals. 8th Ed. National Research Council (US). Washington, D.C. The National Academies Press.
Nyigo VA, Mdegela R, Mabiki F, Malebo HM. 2015. Assessment of dermal irritation and acute toxicity potential of extracts from synadenium glaucescens on healthy rabbits, wistar albino rats and albino mice. European J. Med. Plants, 10(4): 1-11.
OECD. 2017. OECD Guidline For The Testing Of Chemicals 402: Acute Dermal Toxicity-Fixed Dose Procedure. Paris.
Ololade ZS, Abam EO, Anuoluwa IA, Abiona OO. 2020. Secondary metabolites, pharmacognostic and therapeutic activities of the rhizome extract of Curcuma longa grown in South-West, Nigeria. J. Phytopharmacol., 9(1): 30-37.
Paul AJ, Ann M, Goodwin C. 2011. Anatomy and Physiology of the Skin. J. Dermatol. Nurses’ Assoc., 3: 203-213.
Praskash J, Pilerood SA. 2010. Chemical composition and antioxidant properties of ginger root (Zingiber officinale). J. Med. Plants Res., 4(24): 2674–2679.
Salehi B, Zakaria ZA, Gyawali R, Ibrahim SA, Rajkovic J, Shinwari ZK, Khan T, Sharifi-Rad J, Ozleyen A, Turkdonmez E, Valussi M, Tumer TB, Fidalgo LM, Martorell M, Setzer WN. 2019. Piper species: a comprehensive review on their phytochemistry, biological activities and applications. Molecules. 24(7):1364.
Sari AM, Cikta EV. 2016. Ekstraksi flavonoid dari temu ireng (Curcuma Aeruginosa Roxb) dan aplikasinya pada sabun transparan. J. Konversi., 5(1): 17–23.
Sasmito WA, Wijayanti AD, Fitriana I, Sari PW. 2015. The acute toxicity test of herbal medicine in mice based on Organization for Economic Co-operation and Development (OECD). J. Sains Vet., 33(2): 234-239.
Shan CY, Iskandar Y. 2018. Studi kandungan kimia dan aktivitas farmakologi tanaman kunyit (Curcuma longa L.). Farmaka, 16(2): 547-554.
Syahidah A, Saad CR, Hassan MD, Rukayadi Y, Norazian MH, Kamarudin MS. 2017. Phytochemical analysis, identification and quantification of antibacterial active compounds in betel leaves, piper betle methanolic extract. Pakistan J. Biolog. Sci. 20: 70-81.
UN. 2015. Globally Harmonized System of Classification and Labelling of Chemicals (GHS). Fifth Revised Edition, United Nation, New York and Geneva.
Vaughn AR, Branum A, Sivamani RK. 2016. Effects of turmeric (Curcuma longa) on skin health: a systematic review of the clinical evidence. Phytother. Res. 30(8): 1243‐1264.
Published
2020-08-29
How to Cite
MERDANA, I Made et al.
ASSESSMENT OF THE ACUTE DERMAL TOXICITY POTENTIAL OF NATURAL VETERINARY MEDICINE MINYAK RAJAS IN WISTAR RATS.
Buletin Veteriner Udayana, [S.l.], p. 205-214, aug. 2020.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/60806>. Date accessed: 24 apr. 2024.
doi: https://doi.org/10.24843/bulvet.2020.v12.i02.p16.
Section
Articles
