THE INFLUENCE OF PEEL EXTRACT MUSA PARADISIACA FORMATYPICA AGAINST KIDNEY HISTOLOGY, UREUM LEVELS AND CREATININ RATTUS NOVERGICUS AFTER INTENSIVE EXERCISE

  • Putu Oky Astawibawa Mahasiswa Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia
  • I Nyoman Suarsana Laboratorium Biokimia Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia
  • I Gusti Ayu Agung Suartini Laboratorium Biokimia Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia

Abstract

Excessive physical activity triggers the formation of reactive oxygen species (ROS) that can damage the kidneys and other organs. This study aims to determine the effect of banana’s Kepok peel extract on the urea, creatinine, and renal histology levels of rats after intensive training. This study used 27 male white rats weighing 200-225 g. Rats were divided into three treatment groups: control (T0), intensive training (T1), intensive training (T2), and banana’s Kepok peel extract was given for 28 days. Urea samples were analyzed using the Urea Col method and creatinine samples were analyzed using the Jaffe method, while kidney samples were examined using histological preparations using the hematoxylin eosin (HE) staining method. In treatment with T0, urea and creatinine levels of 89,456 ± 2,938 and 0.867 ± 0.07 mg / dl were obtained, respectively; Treatment T1 with urea and creatinine levels 101,144 ± 1,805 and 0.944 ± 0.10 mg / dl, and treatment T2 with levels of urea and creatinine were 99,889 ± 4,075 and 0.900 ± 0.00 mg / dl, respectively. Observation of renal histology at T1 found degeneration and necrosis. T2 shows a decrease in degeneration and necrosis accompanied by an increase in regeneration of the epithelium of proximal and distal tubular cells. Supply of banana’s Kepok peel extract using a dose of 1 cc / kg bw. Urea levels in the T1 and T2 treatment groups differed significantly from T0, but when viewed from the mean standard deviation, it showed that T2 was approaching T0 levels. Creatinine T2 levels were not different from T0 and T1, while T0 was different from T1. Additionally, banana’s Kepok peel extract can prevent damage to kidney tissue but has not been significantly different.

Downloads

Download data is not yet available.

References

Agarwal S, Sharma S, Agrawal V, Roy N. 2005. Caloric restriction augments ros defense in S. cerevisiae, Byasir2p independent mechanism. Free Radical Res. 39(1): 55-62.
Arifin H, Delvita V, Almahdy. 2007. Pengaruh pemberian vitamin C terhadap fetus pada mencit diabetes. J. Sains Teknol. Farmasi. 12(1):
Atun S, Arianingrum R, Handayani S, Rudyansah, Garson M. 2007. Identifikasi dan uji aktivitas antioksidan senyawa kimia dari ekstrak metanol kulit buah pisang (Musa paradisiaca Linn.). Indo. J. Chem. 7(1): 83-87.
Cheville NF. 2006. Introduction to Veterinary Pathology, 3rd Ed. Ames (US): Blackwell Publishing.
Cotran RS, Rennke H, Kumar V. Buku Ajar Patologi. 7th Ed. Jakarta: EGC, 2007.
Da’I M, Triharman F. 2010. Uji aktivitas penangkap radikal DPPH (1,1-Difenil-2-Pikrilhidrazil) isolat alfa mangostin kulit buah manggis (Garcinia mangostana L.). Pharmacon. 11(2): 47-50.
Evans GW, Secker R. 2004. Motivational consequences of environmental stress. J. Environ. Psychol. (24): 143-265.
Fitrianingsih SP, Purwanti L. 2012. Uji efek hipoglikemik ekstrak air kulit buah pisang ambon putih [Musa (AAA Group)] terhadap mencit model hiperglikemik galur swiss webster. Proc. Seminar Nasional Penelitian dan PKM. 3(1): 73-80.
Khaira K. 2010. Menangkal radikal bebas dengan anti-oksidan. program studi tadris matematik STAIN Batu Sangkar. J. Saintek. 2(2): 183-187.
Kiyatno. 2009. Antioksidan vitamin dan kerusakan otot pada aktivitas fisik studi eksperimen pada mahasiswa JPOK-FKIP UNS Surakarta. M. Med. Indon. 6(43).
Meyer DJ, Harvey JW. 2004. Veterinary Laboratory Medicine Interpretation and Diagnosis, Philadelphia: Saunders.
Polcomy J, Yanishlieva N, Gordon M. 2001. Antioxidants in food, Practical applications, Wood Publishing Limited, Cambridge, England
Robbins SL, Kumar V. 2007. Buku ajar patologi anatomi. Edisi ke-6. Jakarta (ID): EGC. Pp. 113, 572, 595-597.
Rosdiana, R. 2014. Fortifikasi tahu menggunakan antioksidan dari ekstrak kulit pisang kepok (Musa Bluggoe). Respositpry UPI, Universitas Pendidikan Indonesia Bandung.
Sinaga FA. 2016. stress oksidatif dan status antioksidan pada aktivitas fisik maksimal. J. Generasi Kampus. 9(2): 176-189.
Someya S, Yoshiki Y, Okubo K. 2002. Antioxidant compounds from bananas (Musa Cavendish). Food Chem. 79(3): 351-354.
Suhita, NLPR, Sudira,W, Wanaya, I,B,O, 2013, Histopatologi ginjal tikus putih akibat pemberian ekstrak pegagan (Contella asiatica) peroral. Bul. Vet. Udayana. 5(2): 71-78.
Supriyanti FMT, Suanda H, Rosdiana R. 2015. Pemanfaatan ekstrak kulit pisang kepok (musa bluggoe) sebagai sumber antioksidan pada produksi tahu. Proc. Seminar Nasional Kimia dan Pendidikan Kimia VII, Universitas Sebelas Maret Surakarta, Pp. 393-400.
Susetyarini E. 2007. Pengaruh dekok daun beluntas (pluchea indica less) terhadap ld 50 (toksisitas akut) tikus putih jantan (Ratus nurwegicus). Laporan Penelitian. Lemlit UMM.
Togatorop D, Pasiak TF, Wongkar D, Kasek MM. 2016. Gambaran histologik ginjal tikus wistar yang diberikan jus tomat setelah diinduksi dengan monosodium glutamat. J. e-Biomedik. 4(2).
Treml J, Smejkal K. 2016. Flavonoids as potent scavengers of hydroxyl radicals. Comp. Rev. Food Sci. Food Safety. 15: 720-738.
Valko M, Leibfritz D, Moncol J, Cronin MTD, Mazur M, Telser J. 2007. Review: Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell. Biol. 39: 44-84.
Verma P, Paswan SK, Verma S, Singh SP, Rao CV, Shrivastva S, Gupta RK. 2017. Assessment of hepatoprotective activity of Musa paradisica linn. Whole plant extract against carbon tetrachloride induced hepatotoxicity in wistar rats. IJPSR. 8(1): 126-131.
Wahdaningsih S, Setyowati EP, Wahyuono S. 2011. Aktivitas penangkap radikal bebas dari batang pakis (Alsophila glauca J. Sm). Maj. Obat Tradisional. 16(3): 156-160.
Widhyari, S, D., Esfandiari, A., Cahyono A D. 2015. Profil kreatinin dan nitrogen urea darah pada anak sapi friesian holstein yang disuplementasi Zn. Acta Vet. Indon. 3(2): 45-50
Wresdiyati T. 2003. Immunohistochemical study of oxygen-free radical scavenger superoxide dismutase (Cu,Zn-SOD) in the liver of rats under stress condition. Biota. 8(3): 107–112.
Wresdiyati T, Mamba K, Adnyane IKM, Aisyah US. 2002. The effect of stress condition on the intracellular antioxidant copper,zinc-superoxide dismutase in the rat kidney: an immunohistochemical study. Hayati. 9(3): 85-88.
Published
2022-01-29
How to Cite
ASTAWIBAWA, Putu Oky; SUARSANA, I Nyoman; SUARTINI, I Gusti Ayu Agung. THE INFLUENCE OF PEEL EXTRACT MUSA PARADISIACA FORMATYPICA AGAINST KIDNEY HISTOLOGY, UREUM LEVELS AND CREATININ RATTUS NOVERGICUS AFTER INTENSIVE EXERCISE. Buletin Veteriner Udayana, [S.l.], p. 578-585, jan. 2022. ISSN 2477-2712. Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/60269>. Date accessed: 28 mar. 2024. doi: https://doi.org/10.24843/bulvet.2022.v14.i05.p18.
Section
Articles