THE EFFECT OF DERMAL APPLICATION OF BINAHONG LEAF EXTRACT OINTMENT ON INCISION WOUND

  • Putu Oka Samirana Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana Jl. Raya Kampus Unud, Bukit Jimbaran, Badung-Bali
  • Luh Made Sudimartini Departemen Klinik Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. P.B. Sudirman, Dangin Puri Klod, Denpasar-Bali;
  • I Wayan Juli Sumadi Departemen Patologi Anatomi, Fakultas Kedokteran, Universitas Udayana Jl. P.B. Sudirman, Dangin Puri Klod, Denpasar-Bali
  • Putu Dessy Wilantari Program Studi Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana Jl. Raya Kampus Unud, Bukit Jimbaran, Badung-Bali

Abstract

Incision wound are injuries that caused by sharp instruments, for example wounds that occur after surgery. This study aims to determine the effect of Binahong leaf extract ointment on incision wound healing. Anredera scandens (L.) Moq. extracted using ethanol 70%, then made an ointment on the basis of adeps lanae and vaselin album. A total of 35 female white wistar rats aged 2-3 months with a weight of 200 g and acclimatized for one week, arranged to 7 the tratments groups. All groups made incision wounds on the back parallel to the vertebrae. Everyday given 250 mg of ointment twice a day. After given the ointment, then parameters observed from histopathology of the skin were infiltration of inflammatory cells and the formation of collagen. The results showed that on the the 21st were still inflammation cells that were not significantly different (p>0,05) in all groups and at 40% extract concentrations a significantly different formation of collagen occured (p<0,05). In conclusion, the ethanolic leaf extract of A. scandens (L.) Moq. not able to reduce inflammation cells after 21 days of treatment and at a concentration of 40% showed the most significant collagen formation.

Downloads

Download data is not yet available.

References

Ahmad AR, Juwita J, Ratulangi SAD, dan Malik A. 2016. Penetapan Kadar Fenolik dan Flavonoid Tital Ekstrak Metanol Buah dan Daun Patikala (Etlingera Elatior (Jack) Rm Sm) Menggunakan Spektrofotometri Uv-Vis. Pharmaceutical Sciences and Reserach (Psr) 2(1): 1-10.
Ansel HC. 2008. Pengantar Bentuk Sediaan Farmasi. Edisi IV. Jakarta. UI Press.
Berata IK, Winaya IBO, Adi AAAM, dan Adnyana IBA. 2011. Buku Ajar Patologi Veteriner Umum. Denpasar. Swasta Nulus.
Celloti F and Laufer S. 2001. Inflammation, Healing and Repair Synopsis. J. Phar. 43(5): 2001.
Depkes RI. 2014. Farmakpe Indonesia. Edisi V. Jakarta. Departemen Kesehatan Republik Indonesia.
Kementerian Kesehatan RI. 2011. Farmakope Herbal Indonesia. Edisi I.
Jakarta. Departemen Kesehatan Republik Indonesia.
Mahmood, A.., A. K. Tiwari, O. Kucuk, dan S. Ali. 2016. Triterpenoid saponin-rich fraction of Centella asiatica decreases IL-1β and NF-kB, and augments tissue regeneration and excision wound repair. Turkish Journal of Biology. 40: 399-409.
Miladiyah I. dan Prabowo BR. 2012. Ekstrak Etanol daun Anreder cordifolia (Ten.) Steenis (Basallaceae) memperbaiki Penyembuhan Luka Pada Marmut. Univ Med. 31(1): 4-11.
Myers WT., Leong M., Philips LG. 2007. Optimizing The Patient fo Surgical Treatment of The Wound. Clin Plast Surgery. 34(4): 607-627.
Nayak, B. S. and L. M. P. Pereira. 2006. Catharanthus Roseus Flower Extract has Wound-Healing Activity in Sprague Dawley Rats. BMC Complementary and Alternative Medicine. 6(41): 2.
Plumb DC. 2008. Veterinary Drug Handbook. Blackwell Publishing.
Prasetyono, T. O. H. 2009. General Concept of Wound Healing. Med J Indones. 18(3): 208-216.
Samirana PO, Swastini DA, Subratha IDGPY, dan Ariadi KA. 2016. Uji
Aktivitas Penyembuhan Luka Ekstrak Etanol Daun Binahong (Anredera
scandens (L.) Moq.) pada Tikus Betina Galur Wistar. Jurnal Farmasi
Udayana. 5(2): 19-23.
Samirana PO, Swastini DA, Ardinata IPR, dan Suarka IPSD. 2017. Penentuan Profil Kandungan Kimia Ekstrak Etanol Daun Binahong (Anredera scandens (L.) Moq.). Jurnal Farmasi Udayana. 6(1): 23-33.
Sastrawan NKL, Wardhita AAGJ, Dada IKA, dan Sudimartini LM. 2016. Perbandingan Kecepatan Kesembuhan Luka Insisi yang Diberi Amoksisilin-Deksametason dan Amoksisilin-Asam Mefenamat pada Tikus Putih (Rattus norvegicus). Indonesia Medicus Veterinus. 5(2): 129-144.
Semer, N. B. 2013. Dasar-dasar Perawatan Luka. Jakarta: Global-HELP Organization.
Tsala DE, Amadou D, and Habtemariam S. 2013. Natural Wound Healing dan
Bioactive Natural Products. Phytopharmacology 4(3): 532-560.
WHO. 2013. Recommendation on Postnatal Care of The Mother and Newborn. WHO. Department of Maternal, Newborn, Child and Adolescent Health.
Yadnya-Putra, A. A. G. R, P. O. Samirana, dan D. A. A. Andhini. 2019. Isolasi dan Karakterisasi Senyawa Flavonoid Potensial Antioksidan dari Daun Binahong (Anredera scandens (L.) Moq.). Jurnal Farmasi Udayana. 8(2): 85-94.
Published
2022-01-29
How to Cite
SAMIRANA, Putu Oka et al. THE EFFECT OF DERMAL APPLICATION OF BINAHONG LEAF EXTRACT OINTMENT ON INCISION WOUND. Buletin Veteriner Udayana, [S.l.], p. 185-196, jan. 2022. ISSN 2477-2712. Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/56717>. Date accessed: 09 oct. 2024. doi: https://doi.org/10.24843/bulvet.2022.v14.i02.p16.
Section
Articles