THE EFFECTIVENESS OF NEEM LEAF ETHANOL EXTRACT ON THE IN VITRO OF RHIPICHEPALUS SANGUINEUS

  • I Made Merdana Laboratory of Veterinary Pharmacology and Pharmacy, Faculty of Veterinary Medicine Udayana University, Jl. PB. Sudirman Denpasar Bali, Indonesia 80225.
  • Ida Ayu Pasti Hapsari Laboratory of Veterinary Parasitology, Faculty of Veterinary Medicine, Udayana University, Jl. PB. Sudirman Denpasar Bali, Indonesia 80225.
  • Fuady Muslih Student of Veterinary Internship Program Udayana University

Abstract

The Neem leaves were very potential to developed as an anti-ectoparasite drug in pets. The purpose of this research was to determine the effectiveness of ethanol extract of Neem leaves (Azadirachta indica, A. Juss) against Rhipicephalus sanguineus dog by in vitro. Using a completely randomized design, 250 ticks were randomly divided into 5 groups with five repetitions. A gentle spray treatment of neem leaves extract, sequentially; P0 as negative control with Aquadest, P1 as positive control with 0.5% deltamethrin, P2 with 2.5% Neem leaves extract, P3 with 5% Neem leaves extract, P4 with 10% Neem leaves extract, Each group consists of 12 ticks that placed in petri dish with a cotton pad that has been sprayed with each solvent, then treated again by spraying it gently and equal. The results of the research showed that the ethanol extract of neem leaves proved to be very significant (P <0.01) against the mortality of R. sanguineus by in vitro. This research, solvent with 10% of neem leaves extract was most effective to exterminate R. sanguineus ticks with 100% mortality.

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Published
2020-02-28
How to Cite
MERDANA, I Made; HAPSARI, Ida Ayu Pasti; MUSLIH, Fuady. THE EFFECTIVENESS OF NEEM LEAF ETHANOL EXTRACT ON THE IN VITRO OF RHIPICHEPALUS SANGUINEUS. Buletin Veteriner Udayana, [S.l.], p. 86-91, feb. 2020. ISSN 2477-2712. Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/56278>. Date accessed: 01 oct. 2020. doi: https://doi.org/10.24843/bulvet.2020.v12.i01.p15.
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Articles

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