PHYLOGENY ANALYSIS OF BALI ISOLATES OF NEWCASTLE DISEASE VIRUS IN 2013 TO 2014 BASED ON CLEAVAGE SITE OF THE FUSION PROTEIN
Abstract
Newcastle disease is a contagious disease caused by the Avian paramyxovirus serotype 1 virus, which infected the poultry. The aims of this study were conducted to phylogenetic analysis of the fusion protein cleavage site of Newcastle disease virus found in a chicken farm in the province of Bali throughout the year 2013 to 2014. There are four isolates from sick chicken’s cases/death suspected of being infected by the Newcastle disease virus. The sequence of the protein fusion was aligned and edited using MEGA5. The amino acid sequence of the F cleavage site of all isolates was 112R-R-Q-K-R-F117 and analysis of phylogeny tree, which justified that all four are virulent of Newcastle disease virus. It is concluded that four Bali isolates in 2013 to 2014 under this study are Newcastle disease virus of genotype VII.
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References
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de Leeuw OS, Hartog L, Koch G, Peeters BPH. 2003. Effect of fusion protein cleavage site mutations on virulence of Newcastle disease virus: non-virulent cleavage site mutants revert to virulence after one passage in chicken brain. J. General Virol. 84: 475–484.
de Leeuw OS, Koch G, Hartog L, Ravenshort, N, Peeters, BPH. 2005. Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin–neuraminidase protein. J. General Virol. 86: 1759–1769.
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Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28(10): 2731–2739.
Wicaksana IGHP, Adi AAAM, Kardena IM. Pathological lesions in chicken embryo caused by newly virulent isolate of newcastle disease virus. J. Vet. 20(3): 337-344.
Yeo SG, Nagy E, Krell PJ. 2003. Indirect method for prediction of hemagglutination inhibition antibody titers to Newcastle disease virus in chickens by titration of antibodies in egg yolk. J. Vet. Diagn. Invest. 15: 184–187.
Yusoff K, Tan WS. 2001. Newcastle disease virus: macromolecules and opportunities. Avian Pathol. 30: 439– 455.
Zhang S, Wang X, Zhao C, Liu D, Hu Y. 2011. Phylogenetic and pathotypical analysis of two virulent newcastle disease viruses isolated from domestic ducks in China. PLoS ONE. 6(9): e2500.
Adi AAAM, Astawa NM, Putra KSA, Hayashi Y, Matsumoto Y. 2010. Isolation and characterization of a pathogenic newcastle disease virus from a natural case in Indonesia. J. Vet. Med. Sci. 72(3): 313–319.
Aldous EW, Mynn JK, Banks J, Alexander, DJ. 2003. A molecular epidemiological study of avian paramyxovirus type 1 (Newcastle disease virus) isolates by phylogenetic analysis of a partial nucleotide sequence of the fusion protein gene. Avian Pathol. 32(3): 239-257.
Alexander DJ. 1987. Taxonomy and nomenclature of avian paramyxoviruses. Avian Pathol. 16: 547-552.
Collins MS, Bashiruddin JB, Alexander DJ. 1993. Deduced amino acid sequences at the fusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity and pathogenicity. Archives Virol. (128): 363-370.
de Leeuw OS, Hartog L, Koch G, Peeters BPH. 2003. Effect of fusion protein cleavage site mutations on virulence of Newcastle disease virus: non-virulent cleavage site mutants revert to virulence after one passage in chicken brain. J. General Virol. 84: 475–484.
de Leeuw OS, Koch G, Hartog L, Ravenshort, N, Peeters, BPH. 2005. Virulence of Newcastle disease virus is determined by the cleavage site of the fusion protein and by both the stem region and globular head of the haemagglutinin–neuraminidase protein. J. General Virol. 86: 1759–1769.
Dortmans JCFM. 2011. Virulence Determinants of Newcastle Disease Virus. Dissertation. Universitas Utrecht, Nederlands.
Fernandes CC, Varani, AM., Lemos, EGM, de Miranda, VFO, Silva KR, Fernando FS, Montassier MFS, Montassier HJ. 2014. Molecular and phylogenetic characterization based on the complete genome of a virulent pathotype of Newcastle disease virus isolated in the 1970s in Brazil. Infect. Genet. Evol. 26: 160–167.
Glickman RL, Syddall RJ, Iorio RM, Sheehan JP, Bratt MA. 1988. Quantitative basic residue requirements in the cleavage-activation site of the fusion glycoprotein as a determinant of virulence for newcastle disease virus. J. Virol. 62(1): 354-356.
Gong YY, ZhiZhong CUI. 2011. Epitope variation in the Newcastle disease virus HN gene under antibody immune selective pressure in cell culture. Sci China Life Sci. 54(5): 474–479.
Jacobson RH. 1998. Validation of serological assays for diagnosis of infectious diseases. Rev. Sci. Tech. Off. Int. Epiz. 17(2): 469-486.
Kencana GAY. 2012. Penyakit Unggas. Udayana Universiati Press. Pp. 34 – 52.
Kencana GAY, Kardena IM, Mahardika, IGN. 2012. Peneguhan diagnosis penyakit Newcastle disease lapang pada ayam buras di bali menggunakan teknik RT-PCR J. Kedokteran Hewan. 6(1).
Kim SH, Subbiah M, Samuel AS, Collins PL, Samal SK. 2011. Roles of the fusion and hemagglutinin-neuraminidase proteins in replication, tropism, and pathogenicity of avian paramyxoviruses. J. Virol. 85(17): 8582–8596.
Kusmaedi. 2001. Teknik uji hemaglutination inhibition Untuk mengukur tingkat kekebalan terhadap Newcastle disease dan egg drop Syndrome’76. Temu Teknis Fungsional Non Peneliti, Balai Penelitian Veteriner.
Lee DH, Kwon JH, Noh JY, Park JK, Yuk SS, Erdene TO, Nahm SS, Kwon YK, Lee SW, Song CS. 2016. Viscerotropic velogenic Newcastle disease virus replication in feathers of infected chickens. J. Vet. Sci. 17(1): 115–11.
McGinnes LW, Morrison TG. 2003. Inhibition of Receptor Binding Stabilizes Newcastle Disease Virus HN and F Protein-Containing Complexes. J. Virol. 80(6): 2894–2903.
Morrison TG. 2003. Structure and function of a paramyxovirus fusion protein. Biochim. Biophysica Acta. 1614: 73–84.
OIE. 2012. Newcastle Disease. Version adopted by the World Assembly of Delegates, 2: 3-14.
Otim MO, Christensen H, Jørgensen PH, Handberg KJ, Bisgaard M. 2004. Molecular Characterization and Phylogenetic Study of Newcastle Disease Virus Isolates from Recent Outbreaks in Eastern Uganda. J. Clin. Microbiol. 42(6): 2802–2805.
Putra IGAA, Adi AAAM. 2016. Confirmation of avian paramyxovirus type 1 (APMV-1) infection by histopathology, serology and molecular method (Indonesian). J. Ked. Hewan. 10(2): 162-165.
Piacenti AM, King DJ, Seal BS, Zhang J, Brown CC. 2006. Pathogenesis of Newcastle disease in commercial and specific pathogenfree Turkeys experimentally infected with isolates of different virulence. Vet. Pathol. 43: 168-170
Rell F, Adi AAAM, Mahardika IGNK. 2015. Virulensi virus newcastle disease isolat lapang berdasarkan analisis bioinformatika gen protein hemaglutinin – neuraminidase. J. Ilmu dan Kesehatan Hewan. 3(1): 17-28.
Shengqing Y, Kishida N, Ito H, Kida H, Otsuki K, Kawaoka Y, Ito T. 2002. Generation of velogenic newcastle disease viruses from a nonpathogenic waterfowl isolate by passaging in chickens. Virology. 301: 206–211.
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Evol. 28(10): 2731–2739.
Wicaksana IGHP, Adi AAAM, Kardena IM. Pathological lesions in chicken embryo caused by newly virulent isolate of newcastle disease virus. J. Vet. 20(3): 337-344.
Yeo SG, Nagy E, Krell PJ. 2003. Indirect method for prediction of hemagglutination inhibition antibody titers to Newcastle disease virus in chickens by titration of antibodies in egg yolk. J. Vet. Diagn. Invest. 15: 184–187.
Yusoff K, Tan WS. 2001. Newcastle disease virus: macromolecules and opportunities. Avian Pathol. 30: 439– 455.
Zhang S, Wang X, Zhao C, Liu D, Hu Y. 2011. Phylogenetic and pathotypical analysis of two virulent newcastle disease viruses isolated from domestic ducks in China. PLoS ONE. 6(9): e2500.
Published
2021-02-26
How to Cite
RELL, Fedri; ADI, Anak Agung Ayu Mirah; MAHARDIKA, I Gusti Ngurah Kade.
PHYLOGENY ANALYSIS OF BALI ISOLATES OF NEWCASTLE DISEASE VIRUS IN 2013 TO 2014 BASED ON CLEAVAGE SITE OF THE FUSION PROTEIN.
Buletin Veteriner Udayana, [S.l.], p. 67-74, feb. 2021.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/55128>. Date accessed: 21 nov. 2024.
doi: https://doi.org/10.24843/bulvet.2021.v13.i01.p11.
Section
Articles
