DETERMINATION OF PROTEIN CONTENT AND FRACTION OF CRUDE PROTEIN ANTIGEN CYSTICERCUS CELLULOSAE FROM LOCAL ISOLATE
Abstract
Cysticercosis is a parasitic disease caused by tapeworm larvae, called Cysticercus. Cysticercus found in pigs is Cysticercus cellulosae which is the larvae of the tapeworm Taenia solium. The purpose of this study was to determine the levels and fractions of Crude protein antigen C. cellulosae. C. cellulosae used is local isolates obtained from infected pigs originating from Karangasem - Bali. Determination of protein content larvae were performed using an Invitrogen Quibit Fluorometer and determination of protein fractions larvae techniques using SDS-PAGE electrophoresis. The results showed that levels of the protein C. cellulosae from local isolate was 867 µg/ml. As for protein fractionation obtained 6 bands protein with a molecular weight: bands 1 = 120.5 kDa, bands 2 = 99.8 kDa, bands 3 = 60.8 kDa, bands 4 = 47.7 kDa, bands 5 = 35.6 kDa, and bands 6 = 23.3 kDa.
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References
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Cho SY, Kong Y, Kim SI, Kang Y. 1992. Measurement of 150 kDa protein of Taenia solium metacestoda by enzyme-linked immune electrotranfer blot technique. Kor. J. Parasitol. 30(4): 299-307.
Cruz, ME, Schantz, PM, Cruz I. Preux, PM. Banitez, W. Tsang, VC, Fermoso Dumas, M. 1999. Epilepsi and neurocycercosis in an Andean community. Int. J. Epidemiol. 29: 799-803.
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Pinto PS, Vaz AJ, Germano PM, Nakamura PM. 2000. Performance of the ELISA test for swine cysticercosis using antigens of Taenia solium and Taenia crassiceps cysticerci. Vet. Parasitol. 88(1-2): 127-130.
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Sciutto E, Fragoso G, Fleury A. 2000. Taenia solium disease in humans and pigs: an ancient parasitosis disease rooted in developing countries and emerging as a major health problem of global dimensions. Microbs. Infect. 2: 1875-1890.
Subahar R, Hamid A, Purba W, Wandra T, Karma C, Sako Y, Margono SS, Craig PS, Ito A. 2001. Taenia solium infection in Irian Jaya (West Papua), Indonesia: a pilot serological survey of human and porcine cysticercosis in Jayawijaya District. Trans. R. Soc. Trop. Med. Hyg. 95: 388-390.
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Tsang VC, Brand JA, Boyer AE. 1989. An enzyme linked immunoelectro-transfer blot asaay and glycoprotein antigens for diagnosting human cysticercosis (Taenia solium). J. Infect. Dis. 159: 50-59.
Wandra T, Ito A, Yamasaki H, Suroso T, Margono SS. 2003. Taenia solium cysticercosis, Irian Jaya, Indonesia. J. Emerg. Infect. Dis. 9(7): 884-885.
Yong TS, Yeo IS, Seo JH, Chang JK, Jeong GH. 1993. Serodiagnosis of cysticercosis by ELISA-inhibition test using monoclonal antibodies. Kor. J. Parasitol. 32(2): 149-156.
Benitez L, Garate T, Harrison LJ, Kirkham P, Brookes SM, Parkhouse RM. 1996. Cloning and sequencing of the gene encoding the principal 18 kDa secreted antigen of activated onchoephere of Taenia saginata. Mol. Biochem. Parasitol. 78: 265-268.
Cho SY, Kong Y, Kim SI, Kang Y. 1992. Measurement of 150 kDa protein of Taenia solium metacestoda by enzyme-linked immune electrotranfer blot technique. Kor. J. Parasitol. 30(4): 299-307.
Cruz, ME, Schantz, PM, Cruz I. Preux, PM. Banitez, W. Tsang, VC, Fermoso Dumas, M. 1999. Epilepsi and neurocycercosis in an Andean community. Int. J. Epidemiol. 29: 799-803.
Da Silva AD, Quagliato E M, Rossi C L. 2000. A quantitative enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of neurocysticercosis using a purified fraction from Taenia solium cysticerci. Diagn. Microbiol. Infec. Dis. 37(2): 87-92.
Das S, Mahajan RC, Ganguly NK, Sawhney IM, Dhawan V, Malla N. 2002. Detection of antigen B of Cysticercus cellulosae in cerebrospinal fluid for the diagnosis of human neurocysticercosis. Trop. Med. Int. Health. 7(1): 53-58.
Ditjen P3L (Pengendalian Penyakit dan Penyehatan Lingkungan). 2005. Manual pemberantasan penyakit menular. Departemen Kesehatan Republik Indonesia. Online http://www.pppl.depkes.go.id/ (Diakses pada 21 Januari 2008).
EC-HCPDG (European Comission-Health Consumer Protection Directorate General). 2000. Opinion of the scientific commite on veterinary measures relating to public health on the control of taeniasis/cysticercosis in man and animals.
Flisser A, Espinoza B, Tovar A, Plancarte, A, Correa D. 1986. Host-parasite relationship in cysticercosis: immunologic study in different compartment of the host. Vet. Parasitol. 20: 95-102.
Flisser A, Gauci CG, Zoli A. 2004. Induction of protection against porcine cysticercosis by vaccination with recombinant onchosphere antigens. Infect. Immun. 72: 5292-5297.
Gauci CG, Flisser A, Ligtowlers MW. 1998. A Taenia solium onchosphere protein homologous to host-protective Taenia ovis and Taenia saginata 18 kDa antigens. Int. J. Parasitol. 28: 757-760.
Handojo I, Margono SS. 2002. Cacing pita yang penting di Indonesia. Dalam: Buku Parasitologi Kedokteran. Ed. 3. Jakarta: Balai Penerbit FKUI. Pp: 92-96.
Harrison L J, Joshua, G W, Wright SH, Parkhouse, R.M. 1989. Specific detection of circulating surface/secreted glycoproteins of viable cysticerci in Taenia saginata cysticercosis. Parasite Immunol. 11: 351-370.
Husain N, Jyotsna, Bagchi M, Huasain M, Mishra MK, Gupta S. 2001. Evaluation of Cysticercus fasciolaris antigen for immunodiagnosis of neurocysticercosis. Neurol. India. 49(4): 375-379.
Ito A, Sako Y, Ishikawa Y, Nakao M, Nakaya K, Yamasaki H. 2002. Differential serodiagnosis for alveolar echinococcosis by Em18-immunoblot and Em18-ELISA in Japan and China. In P. Craig and Z. Pawlowski (Eds.) Cestode Zoonoses: Echinococcosis and Cysticercosis – An Emergent and Global Problem. IOS Press. Amsterdam. Pp: 147-155.
Ito A, Nakao M, Wandra T. 2003. Human taeniasis and cysticercosis in Asia. Lancet. 362: 1918-1920.
Ito A, Putra MI, Subahar R, Sato MO, Okamoto M, Sako Y, Nakao M, Yamasaki H, Nakaya K, Craig PS, Margono SS. 2002. Dogs as alternative intermediate hosts of Taenia solium in Papua (Irian Jaya), Indonesia confirmed by highly specific ELISA and immunoblot using native and recombinant antigens and mitochondrial DNA analysis. J. Helminthol. 76: 311 – 314.
Ito A, Wandra T, Yamasaki H, Nakao M, Sako Y, Nakaya K, Margono SS, Suroso T, Gauci C, Lightowlers MV. 2004. Cysticercosis/taeniasis in Asia and the Pasific. Vectorborne Zoonotic Dis. 4: 95-107.
Kordafshari S, Hosseini SH, Mesghi B, Youssefi MR. 2010. Comparison of electrophoretic patternof larval stages of taenidae and determination of specific antigens of hydatid cyst by western blotting. Global Vet. 4(6): 601-606.
Kraft R. 2007. Cysticercosis: An emerging parasitic disease. american family physichican. 76: Online at http://www.aafp.org/ (Diakses pada 21 Januari 2008).
Laclette JP, Rodriguez M, Landa A. 1989. The Coexistence of Taenia solium cysticerci and the pig: role of antigen B. Acta Leiden. 57: 115-122.
Laclette JP, Landa A, Arcos L, Willms K, Davis, AE, Shoemaker C B. 1991. Paramyosin is the Schistosoma mansoni (Trematoda) Homologue of Antigen B from Taenia solium (Cestoda). Mol. Biochem. Parasitol. 44: 287-295.
Margono SS, Ito A, Suroso T. 2000. The problem of taeniasis and cysticercosis in Irian Jaya (Papua), Indonesia. Proc. Of the six Asian-Pasific Congress for Parasitic Zoonoses. Pp. 55-64.
Pinto PS, Vaz AJ, Germano PM, Nakamura PM. 2000. Performance of the ELISA test for swine cysticercosis using antigens of Taenia solium and Taenia crassiceps cysticerci. Vet. Parasitol. 88(1-2): 127-130.
Rajshekar, V, Joshi DD, Doanh NQ, Ven DE, Xiaonong Z. 2003. Taenia solium taeniasis/cysticercosis in Asia: epidemiology, impact and issues. Acia. Trop. 87: 53-60.
Sarmoko. 2011. Tinjauan tentang ELISA. Online at: http://sarmoko31. wordpress.com/2011/06/28/tinjauan tentang-elisa. (Diakses pada Feb 23 2013)
Satria A. 2012. ELISA (Enzyme-Linked Immunosorbent Assay). Program Magister Ilmu Biomedik. Fakultas Kedokteran Universitas Brawijaya; Malang.
Sciutto E, Fragoso G, Fleury A. 2000. Taenia solium disease in humans and pigs: an ancient parasitosis disease rooted in developing countries and emerging as a major health problem of global dimensions. Microbs. Infect. 2: 1875-1890.
Subahar R, Hamid A, Purba W, Wandra T, Karma C, Sako Y, Margono SS, Craig PS, Ito A. 2001. Taenia solium infection in Irian Jaya (West Papua), Indonesia: a pilot serological survey of human and porcine cysticercosis in Jayawijaya District. Trans. R. Soc. Trop. Med. Hyg. 95: 388-390.
Swacita IBN, Agustina KK, Polos IW, Fitriani S, Natalia N. 2015. Survei seroprevalensi Taenia solium sistiserkosis di Kabupaten Mimika, Papua. Bul. Vet. Udayana. 7(2): 172-178.
Tsang VC, Brand JA, Boyer AE. 1989. An enzyme linked immunoelectro-transfer blot asaay and glycoprotein antigens for diagnosting human cysticercosis (Taenia solium). J. Infect. Dis. 159: 50-59.
Wandra T, Ito A, Yamasaki H, Suroso T, Margono SS. 2003. Taenia solium cysticercosis, Irian Jaya, Indonesia. J. Emerg. Infect. Dis. 9(7): 884-885.
Yong TS, Yeo IS, Seo JH, Chang JK, Jeong GH. 1993. Serodiagnosis of cysticercosis by ELISA-inhibition test using monoclonal antibodies. Kor. J. Parasitol. 32(2): 149-156.
Published
2019-08-31
How to Cite
NUGRAHA, Leksana Aditya Kris; SWACITA, Ida Bagus Ngurah; APSARI, Ida Ayu Pasti.
DETERMINATION OF PROTEIN CONTENT AND FRACTION OF CRUDE PROTEIN ANTIGEN CYSTICERCUS CELLULOSAE FROM LOCAL ISOLATE.
Buletin Veteriner Udayana, [S.l.], p. 220-228, aug. 2019.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/49591>. Date accessed: 23 apr. 2024.
doi: https://doi.org/10.24843/bulvet.2019.v11.i02.p15.
Section
Articles
