Uji Kepekaan Streptococcus spp. yang Diisolasi dari Penyakit Saluran Pernapasan Kompleks Babi terhadap Kanamycin, Streptomycin dan Doxycycline
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https://doi.org/10.24843/bulvet.2022.v14.i03.p02
Abstrak
Penyakit pernapasan pada babi sering terjadi pada produksi babi modern di seluruh dunia dan sering disebut sebagai penyakit saluran pernapasan kompleks (Porcine Respiratory Disease Complex/PRDC). Penanggulangan penyakit PRDC hanya dapat dilakukan jika agen yang menginfeksi adalah bakteri. Bakteri yang paling sering diisolasi pada penyakit saluran pernapasan kompleks babi adalah Streptococcus spp. Penelitian ini bertujuan untuk mengetahui pola kepekaan dari Streptococcus spp. yang diisolasi dari hewan dengan gejala PRDC terhadap kanamycin, streptomycin dan doxycycline. Isolat diambil dari hasil penelitian kasus PRDC pada babi di daerah kabupaten Tabanan, Gianyar dan Badung. Isolat murni kemudian ditanam dalam agar darah (Blood Agar) dan dilakukan uji kepekaan dengan metode Kirby-Bauer terhadap masing-masing antibiotika. Bakteri dapat bersifat resisten, intermediet dan sensitif terhadap antibiotika yang diujikan. Hal ini menimbulkan munculnya pola kepekaan terhadap antibiotika sehingga kuman resisten akan menyebabkan gagalnya pengobatan. Hasil penelitian menunjukkan isolat Streptococcus ? hemolitik 60% peka terhadap kanamycin, 100% peka terhadap streptomycin, 40% resisten terhadap kanamycin dan 100% resisten terhadap doxycycline. Sedangkan isolat Streptococcus ? hemolitik 100% peka terhadap kanamycin dan streptomycin namun 100% resisten terhadap doxycycline.
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Referensi
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Sharma D, Cukras AR, Roger EJ, Southworth DR, Green R. 2007. Mutational analysis of S12 protein and implications for the accuracy of decoding by the ribosome. J. Mol. Biol. 374(4): 1065-1076.
Soedarmanto I, Pasaribu F, Wibawan IWT, Lämmler C. 1996. Identification and molecular characterization of serological group C streptococci isolated from diseased pigs and monkeys in Indonesia. J. Clin. Microbiol. 34(9): 2201-2204.
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Timmerman T, Dewulf J, Catry B, Feyen B, Opsomer G, de Kruif A, Maes D. 2006. Quantification and evaluation of antimicrobial-drug use in group treatments for fattening pigs in Belgium. Prev. Vet. Med. 74: 251–263.
Tsodikova SG, Labby KJ. 2016. Mechanisms of resistance to aminoglycoside antibiotics: overview and perspectives. Med. Chem. Comm. 7(1): 11-27.
Wertheim HFL, Nghia HDT, Taylor W, Schultsz C. 2009. Streptococcus suis: an emerging human pathogen. Clin. Infect. Dis. 48(5): 617-25.
Yeotaek C, Changin Oh, Kunkyu L, Ki-hyun C. 2017. Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method. Vet. Sci. 18(3): 283-289.
Bochev I. 2007. Porcine respiratory disease complex (PRDC): A review. I. Etiology, epidemiology, clinical forms and pathoanatomical features. Bulg. J. Vet. Med. 3: 131−146.
Bousquet E, Morvan H, Aitken I, Morgan JH. 1997. Comparative in vitro activity of doxycycline and oxytetracycline against porcine respiratory pathogens. Vet. Rec. 141: 37−40.
Christina S. 2012. Streptococcus dysgalactiae: A Practical Summary for Controlling Mastitis. Virginia State University.
Dutkiewicz J, Sroka J, Zając V, Wasiński, B, Cisak E, Sawczyn A, Kloc A, dan Wójcik-Fatla A. 2017. Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part I – Epidemiology. An. Argic. Environ. Med. 24: 638-695.
EMEA. 2003. Committee for Veterinary Medicinal Product Kanamycin. The European Agency for the Evaluation of Medicinal Product Veterinary Medicines and Inspection.
Geffner SDE, Moreno MR, Campillo AMS, Pardo SFJ, Gómez GA, Martínez-Lozano MD. 2001. Streptococcus suis meningitis. An. Med. Intern. 18: 317–318.
Hannan PC, Windsor HM, Ripley PH. 1997. In vitro susceptibilities of recent field isolates of Mycoplasma hyopneumoniae and Mycoplasma hyosynoviae to valnemulin (Econor®), tiamutin and enrofloxacin and the in vitro development of resistance to certain antimicrobial agents in Mycoplasma hyopneumoniae. Res. Vet. Sci. 63: 157−160.
Kotra LP, Jalal H, Shahriar M. 2000. Aminoglycosides: perspectives on mechanisms of action and resistance and strategies to counter resistance. Antimicrobial Agents and Chemotherapy 3249–32560066-4804/00/$04.0010.
Marie J, Morvan H, Berthelot-Hérault F, Sanders-Kempf PI, Gautier-Bouchardon AV, Jouy E, Kobisch M. 2002. Antimicrobial susceptibility of Streptococcus suis isolated from swine in France and from humans in different countries between 1996 and 2000. J. Antimicrob. Chem. 50: 201−209.
MacInnes JI, Gottschalk M, Lone AG, Metcalf DS, Ojha S, Rosendal T, Watson SB, Friendship RM. 2008. Prevalence of Actinobacillus pleuropneumoniae, Actinobacillus suis, Haemophilus parasuis, Pasteurella multocida, and Streptococcus suis in representative Ontario swine herds. Can. J. Vet. Res. 72(3): 242-248.
[NCCLS] National Committee for Clinical Laboratory Standards. 2012. Manual of antimicrobial susceptibility testing. USA: Am. Soc. Microbiol. Pp. 1-241.
O’Sullivan T, Friendship R, Blackwell T, Pearl D, McEwen B, Carman S, Slavić D, Dewey C. 2011. Microbiological identification and analysis of swine tonsils collected from carcasses at slaughter. Can. J. Vet. Res. 75: 106-111.
Palmieri C, Varaldo PE, Facinelli B. 2011. Streptococcus suis, an emerging drug-resistant animal and human pathogen. Front. Microbiol. 2: 1-6.
Pratiwi S, Rahim A, Mirawati T, Ikaningsih S, Ariyani K, Hutabarat T, Suharto R, Sardjito, Subandrio A. 1999. Laporan tahunan hasil uji resistensi bakteri terhadap berbagai antibiotika. Laboratorium Mikrobiologi Klinik Fakultas Kedokteran Universitas Indonesia.
Priadi A, Natalia L, Poernomo S. 2004. Penyakit Glasser’s pada babi di Pulau Batam, Propinsi Riau. JITV. 9(4): 266-271.
Samsuri, Utama IH. 2001. Kepekaan Streptococcus equi sub spp. zooepidemicus terhadap antimikroba kanamisin, streptomisin, ceftriakson, dan danofloksasin. J. Vet. 2(4): 111-115.
Sharma D, Cukras AR, Roger EJ, Southworth DR, Green R. 2007. Mutational analysis of S12 protein and implications for the accuracy of decoding by the ribosome. J. Mol. Biol. 374(4): 1065-1076.
Soedarmanto I, Pasaribu F, Wibawan IWT, Lämmler C. 1996. Identification and molecular characterization of serological group C streptococci isolated from diseased pigs and monkeys in Indonesia. J. Clin. Microbiol. 34(9): 2201-2204.
Suarjana IGK, Widya A. 2012. Karakterisasi molekuler dan uji patogenesitas Streptococcus patogen isolat asal Bali. Bul. Vet. Udayana. 4(1): 1-8.
Suarjana IGK, Besung INK, Tono KPG. 2017. Detection of haemophilus parasuis from the respiratory tract of pigs. J. Vet. Animal Sci. 1(1): 11-14.
Syarif A, Purwantyastuti A, Suharti KS, Bahroelim B, Sulistia GG. 2013. Farmakologi dan Terapi. Edisi 5. Balai Penerbit FKUI: Jakarta.
Timmerman T, Dewulf J, Catry B, Feyen B, Opsomer G, de Kruif A, Maes D. 2006. Quantification and evaluation of antimicrobial-drug use in group treatments for fattening pigs in Belgium. Prev. Vet. Med. 74: 251–263.
Tsodikova SG, Labby KJ. 2016. Mechanisms of resistance to aminoglycoside antibiotics: overview and perspectives. Med. Chem. Comm. 7(1): 11-27.
Wertheim HFL, Nghia HDT, Taylor W, Schultsz C. 2009. Streptococcus suis: an emerging human pathogen. Clin. Infect. Dis. 48(5): 617-25.
Yeotaek C, Changin Oh, Kunkyu L, Ki-hyun C. 2017. Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method. Vet. Sci. 18(3): 283-289.
Diterbitkan
2022-01-29
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PURBA, Dwi Arso; GELGEL, Ketut Tono Pasek; SUARJANA, I Gusti Ketut.
Uji Kepekaan Streptococcus spp. yang Diisolasi dari Penyakit Saluran Pernapasan Kompleks Babi terhadap Kanamycin, Streptomycin dan Doxycycline.
Buletin Veteriner Udayana, [S.l.], p. 202-209, jan. 2022.
ISSN 2477-2712.
Tersedia pada: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/49176>. Tanggal Akses: 21 may 2026
doi: https://doi.org/10.24843/bulvet.2022.v14.i03.p02.
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