EXPRESSION OF GROWTH HORMONE RECEPTOR GENE ON BALI CATTLE AND WAGYU CATTLE MUSCLE
Abstract
This study aims to study the molecular weight and distribution of protein growth hormone receptors (GHR). The sample used was the Balinese beef eye ribs and Wagyu beef muscle. Molecular weight examination using the SDS-PAGE receptor method, Western Blotting, and immunohistochemistry staining. The results showed that the weight of the growth hormone receptor protein was 76 Kdalton in both types of cattle and the average score of IHK for Wagyu beef muscle was 234.5 and in Bali cattle was 199.6. It can be concluded, namely the weight molecule of the growth hormone receptor muscle ribs in both Wagyu and bali cattle with equal distribution.
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References
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Susanto E. 2010. Penggunaan SDS-PAGE untuk karakterisasi raksi protein sebagai alternati metoda identikasi pencemaran daging ke dalam bakso. J. Ternak. 1(01): 1-7.
Ellen CJ. 2012. The basic of Western Blotting. The Anatomical Rec. 295: 369-371.
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Kim SH, Shin YK, Lee KM, Lee JS, Yun SM. 2003. An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific bacgrond staining. Journal of histochemistry and cytochemistry. 51 (1): 131
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Lawrie RA. 2003. Meat Science 5thed. Oxford Univversity of Nottingham. Pp. 200-305.
Lenny A, Marlinda A, Zanil M, Lisnawati, Saptawati B. 2011. Gambaran ekspresi reseptor estrogen β pada angiofibroma nasofaring belia dengan menggunakan pemeriksaan immunohistokimia. ORLI. 41(1): 8-16.
Li J, Danney MJ, Gilmour RS, Fowden. 2002. Central of growth hormone receptor and y insulin-like growth-1 expresion by cartisel in ovine fetal skeletal muscle. J. Physiol. 541(2): 582 – 589.
Listrat A, Hocguetle JF, Picard B, Menissier T, Djiane J, Jammes H. 2005. Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development. J. Reprod. Nutr. Dev. 45(4): 393 – 403.
Marcio A, Robert T, Juliana Z, Antonio S, Luis F. 2016. Higth level of GHR nuclear translocation in skeletal muscle of a hyperplastic transgenic zebrafish. J. Mol. Endocrinol. 56(1): 47-54.
Purwoko A, Sutarno, Etikawati N. 2003. Polimorfisme DNA pada lokus-2 gen hormon pertumbuhan sapi madura. Biodiversitas. 4(1): 7-11.
Schindler, Christian, Levy, David E, Dcker, Thomas. 2007. JAK-STAT signaling from interferons to cytokines. J. Biol. Chem. 282(28): 20059-20063.
Soeparno. 2005. Ilmu Dan Teknologi Daging. Gadjah Mada University Press. Yogyakarta.
Tahrin M, Yang PC. 2012. Western Blot: tecnique, theory, and trouble shooting. Am. J. Med. Sci. 4(9): 429 -433.
Vleuric L, Van Veldhoven PP, Decuypera E, Khun ER. 1996. Distribution of GH receptors in chicken liver. Proc. Int. Symposium on Avian Endocrinology. Chateau Lake Louise, Alberta. Pp. 30.
Zulkharnaim, Jakaria, Noor RR. 2010. Identifikasi keragaman genetik gen reseptor hormon pertumbuhan (GHR/Alu I) pada sapi bali. Media Peternakan. 33(2): 81-87.
Banroft JD, Gable M. 2008. Theory and practice of histological techniques: Immunohistochemical. 5th Ed. Merck.
Besung INK, Watiniasih NL, Mahardika GNK, Agustina KK, Suwiti NK. 2019. Mineral levels of bali cattle (bos javanicus) from four different type of land in different rearing areas. Biodiversitas. 20(10): 2931-
2936.
Dabbs DJ. 2013. Diagnostic immunohistochemistry: Theranostic and genomic app;ication: Techniques of immunohistochemistry: Principles pitfalls, and standarization 4th Ed. United States of Amerirican: Elsevier Saunders. Pp. 1-19.
Susanto E. 2010. Penggunaan SDS-PAGE untuk karakterisasi raksi protein sebagai alternati metoda identikasi pencemaran daging ke dalam bakso. J. Ternak. 1(01): 1-7.
Ellen CJ. 2012. The basic of Western Blotting. The Anatomical Rec. 295: 369-371.
Hames BD, Rickwood. 2004. A Practical Approach: Gel Elektrooresis Protein. Huntington: Robert E. Krieger Publishing Company.
Hediger R. 1990. Assigment of the growth hormone gen lokus to 19q26qter ini cattle and to 11q25qter in sheep by insitu hybridization. Genome. 8: 171-174.
Jiang H, Ge X. 2014. Mechanism of growth hormone stimulation of skeletal muscle growth in cattle. J. Anim. Sci. 12(1): 21-29.
Junqueira MD, Carneiro J. 1980. Basic histology. 2nd Ed. editorial Guanabara koogam, SA. Rio de Jeneiro. Brazil. Pp. 265-277.
Kim SH, Shin YK, Lee KM, Lee JS, Yun SM. 2003. An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific bacgrond staining. Journal of histochemistry and cytochemistry. 51 (1): 131
Kumar GL, Wendelboe HG, Bisgaard K, Boenisch T, Fomilo AD, Stead RH. 2009. Education Guide: Immunohistochemical staining methods 3th edition. California: DACO corporation. Pp.120
Lawrie RA. 2003. Meat Science 5thed. Oxford Univversity of Nottingham. Pp. 200-305.
Lenny A, Marlinda A, Zanil M, Lisnawati, Saptawati B. 2011. Gambaran ekspresi reseptor estrogen β pada angiofibroma nasofaring belia dengan menggunakan pemeriksaan immunohistokimia. ORLI. 41(1): 8-16.
Li J, Danney MJ, Gilmour RS, Fowden. 2002. Central of growth hormone receptor and y insulin-like growth-1 expresion by cartisel in ovine fetal skeletal muscle. J. Physiol. 541(2): 582 – 589.
Listrat A, Hocguetle JF, Picard B, Menissier T, Djiane J, Jammes H. 2005. Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development. J. Reprod. Nutr. Dev. 45(4): 393 – 403.
Marcio A, Robert T, Juliana Z, Antonio S, Luis F. 2016. Higth level of GHR nuclear translocation in skeletal muscle of a hyperplastic transgenic zebrafish. J. Mol. Endocrinol. 56(1): 47-54.
Purwoko A, Sutarno, Etikawati N. 2003. Polimorfisme DNA pada lokus-2 gen hormon pertumbuhan sapi madura. Biodiversitas. 4(1): 7-11.
Schindler, Christian, Levy, David E, Dcker, Thomas. 2007. JAK-STAT signaling from interferons to cytokines. J. Biol. Chem. 282(28): 20059-20063.
Soeparno. 2005. Ilmu Dan Teknologi Daging. Gadjah Mada University Press. Yogyakarta.
Tahrin M, Yang PC. 2012. Western Blot: tecnique, theory, and trouble shooting. Am. J. Med. Sci. 4(9): 429 -433.
Vleuric L, Van Veldhoven PP, Decuypera E, Khun ER. 1996. Distribution of GH receptors in chicken liver. Proc. Int. Symposium on Avian Endocrinology. Chateau Lake Louise, Alberta. Pp. 30.
Zulkharnaim, Jakaria, Noor RR. 2010. Identifikasi keragaman genetik gen reseptor hormon pertumbuhan (GHR/Alu I) pada sapi bali. Media Peternakan. 33(2): 81-87.
Published
2022-01-15
How to Cite
SUWITI, Ni Ketut; SUDIPA, Putu Henrywaesa; SETIASIH, Ni Luh Eka.
EXPRESSION OF GROWTH HORMONE RECEPTOR GENE ON BALI CATTLE AND WAGYU CATTLE MUSCLE.
Buletin Veteriner Udayana, [S.l.], p. 69-78, jan. 2022.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/45255>. Date accessed: 05 nov. 2024.
doi: https://doi.org/10.24843/bulvet.2022.v14.i02.p02.
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