Ekspresi Gen Reseptor Growth Hormon pada Otot Sapi Bali dan Sapi Wagyu
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https://doi.org/10.24843/bulvet.2022.v14.i02.p02
Abstrak
Penelitian ini bertujuan untuk mengetahui berat molekul dan sebaran protein reseptor Growth Hormon (GHR). Sampel yang dipakai adalah otot rib eye sapi bali dan sapi Wagyu. Pemeriksaan berat molekul protein reseptor memakai metode SDS-PAGE, Western Blotting, dan pengecatan Imunohisto kimia. Hasil penelitian menunjukkan, bahwa berat protein reseptor Growth Hormon adalah 76 Kdalton pada kedua jenis sapi tersebut dan rerata nilai IHK-Score untuk otot sapi wagyu adalah 234,5 dan pada sapi Bali adalah 199,6. Dapat disimpulkan, bahwa berat molekul protein reseptor Growth Hormon otot rib eye pada kedua sapi Wagyu dan sapi bali adalah sama dengan sebaran yang tidak sama.
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Referensi
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Besung INK, Watiniasih NL, Mahardika GNK, Agustina KK, Suwiti NK. 2019. Mineral levels of bali cattle (bos javanicus) from four different type of land in different rearing areas. Biodiversitas. 20(10): 2931-
2936.
Dabbs DJ. 2013. Diagnostic immunohistochemistry: Theranostic and genomic app;ication: Techniques of immunohistochemistry: Principles pitfalls, and standarization 4th Ed. United States of Amerirican: Elsevier Saunders. Pp. 1-19.
Susanto E. 2010. Penggunaan SDS-PAGE untuk karakterisasi raksi protein sebagai alternati metoda identikasi pencemaran daging ke dalam bakso. J. Ternak. 1(01): 1-7.
Ellen CJ. 2012. The basic of Western Blotting. The Anatomical Rec. 295: 369-371.
Hames BD, Rickwood. 2004. A Practical Approach: Gel Elektrooresis Protein. Huntington: Robert E. Krieger Publishing Company.
Hediger R. 1990. Assigment of the growth hormone gen lokus to 19q26qter ini cattle and to 11q25qter in sheep by insitu hybridization. Genome. 8: 171-174.
Jiang H, Ge X. 2014. Mechanism of growth hormone stimulation of skeletal muscle growth in cattle. J. Anim. Sci. 12(1): 21-29.
Junqueira MD, Carneiro J. 1980. Basic histology. 2nd Ed. editorial Guanabara koogam, SA. Rio de Jeneiro. Brazil. Pp. 265-277.
Kim SH, Shin YK, Lee KM, Lee JS, Yun SM. 2003. An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific bacgrond staining. Journal of histochemistry and cytochemistry. 51 (1): 131
Kumar GL, Wendelboe HG, Bisgaard K, Boenisch T, Fomilo AD, Stead RH. 2009. Education Guide: Immunohistochemical staining methods 3th edition. California: DACO corporation. Pp.120
Lawrie RA. 2003. Meat Science 5thed. Oxford Univversity of Nottingham. Pp. 200-305.
Lenny A, Marlinda A, Zanil M, Lisnawati, Saptawati B. 2011. Gambaran ekspresi reseptor estrogen β pada angiofibroma nasofaring belia dengan menggunakan pemeriksaan immunohistokimia. ORLI. 41(1): 8-16.
Li J, Danney MJ, Gilmour RS, Fowden. 2002. Central of growth hormone receptor and y insulin-like growth-1 expresion by cartisel in ovine fetal skeletal muscle. J. Physiol. 541(2): 582 – 589.
Listrat A, Hocguetle JF, Picard B, Menissier T, Djiane J, Jammes H. 2005. Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development. J. Reprod. Nutr. Dev. 45(4): 393 – 403.
Marcio A, Robert T, Juliana Z, Antonio S, Luis F. 2016. Higth level of GHR nuclear translocation in skeletal muscle of a hyperplastic transgenic zebrafish. J. Mol. Endocrinol. 56(1): 47-54.
Purwoko A, Sutarno, Etikawati N. 2003. Polimorfisme DNA pada lokus-2 gen hormon pertumbuhan sapi madura. Biodiversitas. 4(1): 7-11.
Schindler, Christian, Levy, David E, Dcker, Thomas. 2007. JAK-STAT signaling from interferons to cytokines. J. Biol. Chem. 282(28): 20059-20063.
Soeparno. 2005. Ilmu Dan Teknologi Daging. Gadjah Mada University Press. Yogyakarta.
Tahrin M, Yang PC. 2012. Western Blot: tecnique, theory, and trouble shooting. Am. J. Med. Sci. 4(9): 429 -433.
Vleuric L, Van Veldhoven PP, Decuypera E, Khun ER. 1996. Distribution of GH receptors in chicken liver. Proc. Int. Symposium on Avian Endocrinology. Chateau Lake Louise, Alberta. Pp. 30.
Zulkharnaim, Jakaria, Noor RR. 2010. Identifikasi keragaman genetik gen reseptor hormon pertumbuhan (GHR/Alu I) pada sapi bali. Media Peternakan. 33(2): 81-87.
Banroft JD, Gable M. 2008. Theory and practice of histological techniques: Immunohistochemical. 5th Ed. Merck.
Besung INK, Watiniasih NL, Mahardika GNK, Agustina KK, Suwiti NK. 2019. Mineral levels of bali cattle (bos javanicus) from four different type of land in different rearing areas. Biodiversitas. 20(10): 2931-
2936.
Dabbs DJ. 2013. Diagnostic immunohistochemistry: Theranostic and genomic app;ication: Techniques of immunohistochemistry: Principles pitfalls, and standarization 4th Ed. United States of Amerirican: Elsevier Saunders. Pp. 1-19.
Susanto E. 2010. Penggunaan SDS-PAGE untuk karakterisasi raksi protein sebagai alternati metoda identikasi pencemaran daging ke dalam bakso. J. Ternak. 1(01): 1-7.
Ellen CJ. 2012. The basic of Western Blotting. The Anatomical Rec. 295: 369-371.
Hames BD, Rickwood. 2004. A Practical Approach: Gel Elektrooresis Protein. Huntington: Robert E. Krieger Publishing Company.
Hediger R. 1990. Assigment of the growth hormone gen lokus to 19q26qter ini cattle and to 11q25qter in sheep by insitu hybridization. Genome. 8: 171-174.
Jiang H, Ge X. 2014. Mechanism of growth hormone stimulation of skeletal muscle growth in cattle. J. Anim. Sci. 12(1): 21-29.
Junqueira MD, Carneiro J. 1980. Basic histology. 2nd Ed. editorial Guanabara koogam, SA. Rio de Jeneiro. Brazil. Pp. 265-277.
Kim SH, Shin YK, Lee KM, Lee JS, Yun SM. 2003. An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific bacgrond staining. Journal of histochemistry and cytochemistry. 51 (1): 131
Kumar GL, Wendelboe HG, Bisgaard K, Boenisch T, Fomilo AD, Stead RH. 2009. Education Guide: Immunohistochemical staining methods 3th edition. California: DACO corporation. Pp.120
Lawrie RA. 2003. Meat Science 5thed. Oxford Univversity of Nottingham. Pp. 200-305.
Lenny A, Marlinda A, Zanil M, Lisnawati, Saptawati B. 2011. Gambaran ekspresi reseptor estrogen β pada angiofibroma nasofaring belia dengan menggunakan pemeriksaan immunohistokimia. ORLI. 41(1): 8-16.
Li J, Danney MJ, Gilmour RS, Fowden. 2002. Central of growth hormone receptor and y insulin-like growth-1 expresion by cartisel in ovine fetal skeletal muscle. J. Physiol. 541(2): 582 – 589.
Listrat A, Hocguetle JF, Picard B, Menissier T, Djiane J, Jammes H. 2005. Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development. J. Reprod. Nutr. Dev. 45(4): 393 – 403.
Marcio A, Robert T, Juliana Z, Antonio S, Luis F. 2016. Higth level of GHR nuclear translocation in skeletal muscle of a hyperplastic transgenic zebrafish. J. Mol. Endocrinol. 56(1): 47-54.
Purwoko A, Sutarno, Etikawati N. 2003. Polimorfisme DNA pada lokus-2 gen hormon pertumbuhan sapi madura. Biodiversitas. 4(1): 7-11.
Schindler, Christian, Levy, David E, Dcker, Thomas. 2007. JAK-STAT signaling from interferons to cytokines. J. Biol. Chem. 282(28): 20059-20063.
Soeparno. 2005. Ilmu Dan Teknologi Daging. Gadjah Mada University Press. Yogyakarta.
Tahrin M, Yang PC. 2012. Western Blot: tecnique, theory, and trouble shooting. Am. J. Med. Sci. 4(9): 429 -433.
Vleuric L, Van Veldhoven PP, Decuypera E, Khun ER. 1996. Distribution of GH receptors in chicken liver. Proc. Int. Symposium on Avian Endocrinology. Chateau Lake Louise, Alberta. Pp. 30.
Zulkharnaim, Jakaria, Noor RR. 2010. Identifikasi keragaman genetik gen reseptor hormon pertumbuhan (GHR/Alu I) pada sapi bali. Media Peternakan. 33(2): 81-87.
Diterbitkan
2022-01-15
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SUWITI, Ni Ketut; SUDIPA, Putu Henrywaesa; SETIASIH, Ni Luh Eka.
Ekspresi Gen Reseptor Growth Hormon pada Otot Sapi Bali dan Sapi Wagyu.
Buletin Veteriner Udayana, [S.l.], p. 69-78, jan. 2022.
ISSN 2477-2712.
Tersedia pada: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/45255>. Tanggal Akses: 09 jan. 2026
doi: https://doi.org/10.24843/bulvet.2022.v14.i02.p02.
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