OVISIDAL ACTIVITY OF EXTRACT ETHANOL NEEM LEAF FOR ASCARIS SUUM
Abstract
The purpose of this research was to determine the ovicidal effect of neem leaves extract (Azadirachta Indica, A. Juss) on the embryo strength of Ascaris suum eggs. This study using a complete random design with treatment groups, namely P0, P1, P2, P3, P4, with four replication. Group P0 was treated with physiological NaCl as a negative (-) and group (P1) was treated with physiologic NaCl + albendazole as the positive control (+), (P2) treated with 10% neem leaf extract. The P3 group was treated with 20% neem leaf extract and the group (P4) was treated with 30% neem leaf extract, which was done by soaking the worm eggs. The observation of embryonic eggs of the worm was done on the 15th, 21st and 30th day. The result showed that the neem leaves extract had a significant effect (P <0,05) on the embrionation of A. suum eggs. Further test using Games-Howel test showed the extract of neem leaves at dose 30% most effective reduced the embrionation of A. suum eggs. It can be concluded that neem leaf extract has ovicidal activity against A.suum eggs with in vitro test.
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References
Agustina KK, Wirata IW, Dharmayudha AAGO, Kardena IM, Dharmawan NS. 2016b. Increasing farmer income by improved pig management systems. Buletin Vet. Udayana. 8(2): 122-127.
Agustina KK, Swacita IBN, Oka IBM, Dwinata IM, Traub RJ, Cargill C, Damriyasa IM. 2017. Reducing zoonotic and internal parasite burdens in pigs using a pig confinement system. Vet. World. 10(11): 1347-1352.
Ardana IBK. 2007. Peran Ovisidal dan Vermisidal Herbal Serbuk Biji Pepaya Matang dalam Pengendalian Infeksi Ascaris suum pada Babi. Disertasi. Program Pasca Sarjana Universitas Udayana, Bali.
Bhadoriya SS, Uplanchiwar V, Mishra V, Ganeshpurkar A, Raut S, Jain KS. 2011. In vitro anthelmintic and antimicrobial potential of flavonoid rich fraction from Tamarindus indica seed coat. Pharmacologyonline 3: 412-420.
Budiasa K, Samsuri, Merdana IM, Sudira IW. 2017. Potensi ekstrak daun mimba (Azadirachta indica) untuk mengendalikan infeksi cacing nematoda pada babi. Prosiding Seminar Nasional Sains dan Teknologi IV.
Faradila A. 2013. Uji daya anthelmintik ekstrak etanol daun beluntas (Pluchea indica less) terhadap cacing gelang (Ascaris suum) secara in vitro. Skripsi. Malang: Universitas Brawijaya.
Fendriyanto A, Dwinata IM, Oka IBM, Agustina KK. 2015. Identifikasi dan prevalensi cacing nematoda saluran pencernaan pada anak babi di Bali. Indonesia Med. Vet. 4(5): 465-473.
Javandira C, Widnyana IK, Suryadaramawan IGA. 2016. Kajian Fitokimia dan Potensi Ekstrak Daun Tanaman Mimba (Azadirachta indica, A. Juss) Sebagai Pestisida Nabati. Prosiding Seminar Nasional. Unmas Denpasar. Pp: 402-406
Kardinan A, Dhalimi A. 2003. Mimba (Azadirachta Indica A. Juss) Multi Manfaat. Perkembangan Teknologi TRO. 15(1): 1-5.
Katzung BG. 2004. Basic & Clinical Pharmacology. 9th. The McGraw-Hill Companies.United State.
Levine ND. 1994. Buku Ajar Parasitologi Veteriner. Yogyakarta. Terjemahan : G. Ashadi. Gadjah Mada University Press. P: 312-314.
Mali RG, Mehta AA. 2008. A review on anthelmintics plants. Nat. Prod. Radiance. 7(5): 466-475.
Ndjonka D, Agyare C, Lüersen K, Djafsia B, Achukwi D, Nukenine EN, Hensel A, Liebau E. 2011. In vitro activity of cameroonian and ghanaian medicinal plants on parasitic (Onchocerca ochengi) and free-living (Caenorhabditis elegans) nematodes. J. Helminth. 85: 304–312.
Soulsby EJL. 1982. Helminths, Arthrophods and Protoza of Domesticated Animals 7th Ed. Bailliere Tindall. London.
Susanti AE, Prabowo A. 2015. Potensi pinang (Areca catechu) sebagai untuk ternak. the potency of betel nut (Areca catechu) as an anthelmintic for Livestock
Syukron MU, Damriyasa IM, Suratma NA. 2014. Potensi serbuk daun kelor (Moringa oleifera) sebagai anthelmintik terhadap infeksi Ascaris suum dan feed supplement pada babi. Jurnal Ilmu dan Kesehatan Hewan, 2 (2); 89-96.
Ulya N, AT Endharti, R Setyohadi. 2014. Uji daya anthelmintik ekstrak etanol daun kumis kucing (Orthosiphon aristatus) sebagai anthelmintik terhadap Ascaris suum secara in vitro. Majalah Kesehatan FKUB 1 (3): 130-136
Yasa R, Guntoro S. 2004. Prevalensi infeksi cacing gastrointestinal pada babi (studi kasus pada pengkajian pengemukan babi) di Desa Sulahan Kecamatan Susut Kabupaten Bangli. Balai Pengkajian Teknologi Pertanian, Bali.
