HISTOPHATOLOGICAL CHANGES IN HEPAR OF WHITE RATS GIVEN ETHANOL EXTRACT OF ANT NEST AND GENTAMICIN
Abstract
This study aims to evaluate that the administration of ant nest reduces the side effects of toxic doses of gentamicin seen from the histopathological changes in hepar of white rats (Rattus norvegicus). This study used 24 male white rats, divided into four treatment groups, namely negative controls (P0) there were only given food and drink, positive controls (P1) administered gentamicin at a dose of 100mg/kgBB intraperitoneally for 10 days. The group (P2) was given gentamicin 100mg/kgBB intraperitoneally and ant nest extract with a dose of 250mg/kgBB orally for 10 days. The group (P3) was given 250mg/kgBB of ant nest extract for 7 days, after which ant nest extract was given at a dose of 250mg/kgBB and gentamicin with a dose of 100mg/kgBB intraperitoneally for 10 days.White rat hepar was taken and processed for making histopathological preparations, with hematoxylin eosin (HE) staining. The results showed that there was a significant difference between negative control and positive control groups. The mean score of improvement in hepar damage of white rats was significantly different between treatment P1, P2 and P3. The P2 and P3 groups that showed the most change near the mean score of the negative control group. Giving ant nest extracts to white rats for 17 days at a dose of 250mg/kgBB can improve the side effects of the administration of gentamicin 100mg/kgBB in the hepar of white rats.
Downloads
References
Hendarsula RA. 2011. Efek immunostimulan ekstrak umbi sarang s (mymercodia archoldiana) pada tikus putih jantan. Skripsi. Departemen Farmasi FMIPA UI.
Katzung BG. 2010. Farmakologi Dasar dan Klinik (terjemahan), Ed.10 Penerbit Buku Kedokteran EGC, Jakarta.
Khan M, Badar I, Siddiquah A. 2011. Prevention of hepatorenal toxicity with Sonchusasper in gentamicin treated rats. Med. J. 11:1-9.
Kristina, D. 2008. Efek antiinflamasi ekstrak etanol umbi sarang semut (Mymercodia pendans Merr. & Perry) pada tikus (Rattus norvegicus L). Skripsi Jurusan Biologi FMIPA Universitas Surakarta.
Manullang DH, Sudira IW, Berata IK, MErdana IM. 2018. Ekstrak etanol sarang semut menyebabkan kerusakan struktur histologi ginjal mencit. Buletin Veteriner Udayana. 10(2): 183-189.
Pimopalpan P, Saruth N, Rapepol B. 2009. Hepatoprotective potential of extract from seeds of areca catechu and nutgalls of quercus infectoria. J. Mol. 14(12): 4987-5000.
Robbins SL, Kumar V, Cotran SR. 2007. Buku ajar patologi Robbins Ed 7. Jakarta: ECG.
Sada JT, Tanjung RHR. 2010. Keragaman tumbuhan obat tradisional di kampung Nansfori Distrik Supiori Utara, Kabupaten Supiori–Papua. J. Biologi Papua. 2(2): 39-46.
Soewartoyo, Soetopo T. 2009. Potensi sumber daya alam dan peningkatan kualitas sumber daya manusia di kawasan masyarakat pesisir, Kabupaten Bangka. J. Kependudukan Indonesia. 4(2): 61-78.
Subroto MA, Saputro H. 2006 Gempur penyakit dengan sarang semut. Penebar Swadaya. Jakarta.
Tanti AS. Rima M. Peppy KW, Yuyun PJ. 2014. The protective effect sarang semut (myrmecodia tuberose) tubers infusion on gentamicin-piroxicam induced Nephrotoxicity in Rats. Indonesian J. Pharm. 25(2): 91–97.
