DETECTION OF STAPHILOCOCCUS SP. FROM THE PIG RESPIRATORY TRACT

  • Makselina Ayu Dwi Purwanti Praktisi Dokteran Hewan di Denpasar-Bali
  • I Nengah Kerta Besung Faculty of Veterinary Medicine Udayana University
  • I Gusti Ketut Suarjana Faculty of Veterinary Medicine Udayana University

Abstract

This research was conducted for the detection of Staphylococcus sp. in the respiratory tract of pigs. Samples differentiated by sex (male pigs and female pigs). A total of 100 nasal swab samples were collected aseptically from pigs showing symptoms of respiratory disease, from pig farms in the area of Badung and Tabanan. All samples were cultured on to Blood Agar for 18-24h at 37°C. Suspected colonies were then Gram staining, primary tests (tested for catalase and oxidase) and series of biochemical tests. Statistical analysis (Chi-square) was performed to explore the differences in positive samples numbers of Staphylococcus sp. isolated between male and female pigs. Of the 100 samples, seven positive samples of Staphylococcus sp. (six of the boars and one from the female pig). Statistically, the number of boars infected with Staphylococcus sp. higher (P<0.01) than with female pigs Staphylococcus species isolated were: Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus saprophyticus, respectively.

Downloads

Download data is not yet available.

Author Biographies

I Nengah Kerta Besung, Faculty of Veterinary Medicine Udayana University

Laboratory of Veterinary Bacteriology Udayana University

I Gusti Ketut Suarjana, Faculty of Veterinary Medicine Udayana University

Laboratory of Veterinary Bacteriology Udayana University

References

Agustina KK. 2013. Identifikasi dan Prevalensi Cacing Type Strongyle pada Babi di Bali. Bul. Vet. Udayana. 5(4): 131-138.
Agustina KK, Wirata IW, Dharmayudha AAGO, Kardena IM, Dharmawan NS. 2016. Increasing farmer income by improved pig management systems. Bul. Vet. Udayana. 8(2): 122-127.
Agustina KK, Sari PH, Suada IK. 2017. The influence of immersion into Indonesian bay leaf infusion to the quality and durability of pork. Bul. Vet. Udayana. 9(1): 34-41.
Andresen LO, Peter H, Lise D, and Vivi BH. 2004. Exudative Epidermitis in Pigs Caused by Toxigenic Staphylococcus Chromogenes. Veterinary Microbiology. 105:291-300.
Ardana IBK. 2012. Penurunan Angka Morbiditas Dan Mortatiltas Anak Babi Yang Diberi Vitamin Dan Elektrolit Melalui Air Minum Saat Disapih. Bul. Vet. Udayana. 4(1): 33-40.
Badan Pusat Statistika. 2016. Provinsi Bali Dalam Angka 2016. Badan Pusat Statistika Provinsi Bali.
Baele M. 2001. The Gram-positive tonsillar and nasal flora of piglets before and after weaning. J. Appl. Microbiol. 91: 997-1003.
Berkeley, R, C, W., Colman, G., Fox, E., Gross, R., Holmes, B., Jenkins, P, A., Jones, D., Lea, J, V., Marples, R, R., Schofield, G, M., Scraton, M., Skirrow, M, B., Slack, M, P, E., Snell, J, J, S., and Willis, A, T. 1993. Cowan and Steel’s Manual for The Identification of Medical Bacteria. 3rd Ed. Cambridge University Press. USA.
Casanova C, Lukas I, Niklaus VS, Sara D, and Parham S. 2011. Staphylococcus hyicus Bacteremia in A Farmer. J. Clin. Microbiol. 49: 4377-4378.
Chotiah S. 1996. Isolasi, Identifikasi dan Penentuan Serotipe Isolat Pasteurella multocida dari Lesi Pneumonik Paru-Paru Babi dan Kepekaannya Terhadap Beberapa Macam Antibiotika. J. Ilmu Ternak dan Vet. 2: 198-203.
Denis O, Carl S, Marie H, Boudewijn C, Ilse R., Marc D, Glenda W, Bart G, Patrick B, and Marc JS. 2009. Resistant Staphylococcus aureus ST398 In Swine Farm Personel Belgium. Emerg. Infect. Dis. 15:1098-1101.
Gotz F, Bannerman T, and Schleifer KH. 2006. The genera Staphylococcus and Macrococcus. The Prokaryotes 4: 5-75.
Hajek V. 1996. Staphylococcus saprophyticus subsp bovis subsp. Nov Isolated from Bovine Nostrils. Int. J. Systematic Bacteriol. 46:792-796.
Harris LG, Foster SJ, and Richards RG. 2002. An Introduction to Staphylococcus aureus, And Techniques for Identifying and Quantifying S. Aureus Adhesins In Relation To Adhesion To Biomaterials: Review. Europ. Cells and Materials. 4: 39-60.
Iskandar A, Suganda T, dan Lelly Y. 2015. Hubungan Jenis Kelamin dan Usia Anak Satu Tahun Sampai Lima Tahun dengan Kejadian ISPA. Global Med. Health Comm. 3: 1-6.
Kardena IM, Suarjana IGK, dan Udayani P. 2012. Studi Kasus Perhitungan Tingkat Morbiditas, Mortalitas, dan Fatalitas Kolibasilosis pada Babi yang Dipelihara Semi Intensif. Bul. Vet. Udayana. 4(1): 17-22.
Quinn et al., 2002. Veterinary Microbiology and Microbial Disease. Lowa state University press. USA.
Ribet D and Pascale C. 2015. How Bacterial Pathogens Colonize Their Hosts and Invade Deeper Tissues. Microbes and Infect. 17: 173-183.
Siagian PH, Priyanto R, dan Sembiring R. 2004. Kualitas Daging Babi Dengan Pemberian Zeolit dan Tepung Darah Sebagai Sumber Protein dalam Ransum. Media Peternakan. 27(1): 1-11.
Smith EA, Sandeep RPK, Jagadeeswaran D, Thomas EC, Tanya L, Mike M, Subbiah E, and Isis KM. 2011. A Time Course for Suspectibility To Staphylococcus aureus Respiratory Infection During Influenza in A Swine Model. Hindawi Pub. Corp. 1-10.
Vasconcelos NG and Maria LRSC. 2010. Staphylococcal Enterotoxin: Molecular Aspects and Detection Methods. J. Pub Health and Epidemiol. 2(3): 29-42.
Zuk M, and Andrew MS. 2010. Sex Hormones and Immunity to Infection. 1st Ed. Springer. Berlin.
Published
2018-08-27
How to Cite
PURWANTI, Makselina Ayu Dwi; BESUNG, I Nengah Kerta; SUARJANA, I Gusti Ketut. DETECTION OF STAPHILOCOCCUS SP. FROM THE PIG RESPIRATORY TRACT. Buletin Veteriner Udayana, [S.l.], p. 201-207, aug. 2018. ISSN 2477-2712. Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/38089>. Date accessed: 13 july 2024. doi: https://doi.org/10.24843/bulvet.2018.v10.i02.p15.
Section
Articles