ANTIBIOTIC RESISTANCE TO ESCHERICHIA COLI ISOLATED FROM LAYING HENS
Abstract
The study aim was to determine the resistance of Escherichia coli to antibiotics that isolated in laying hens. A total of 100 samples of chicken feces were taken from Tabanan, Bangli and Karangasem districts. E. coli were isolated and identified by Carter (1990) method, followed by a bacterial susceptibility test with agar diffusion method from Kirby Bauer against antibiotics namely oxytetracycline (30 µg), ampicillin (10 µg), streptomycin (10 µg), sulfamethoxazole (30 µg), chloramphenicol (30 µg), and kanamycin (30 µg). The obtained data compared to the next sensitivity standard of the bacteria that classified as sensitive, intermediate, and resistant. The results showed that E. coli from chicken feces are sensitive to kanamycin, whereas the other antibiotics are intermediate or resistant. The increase in resistance is in line to the age of the chicken.
Downloads
References
Bauer AW, Kirby WM, Sherris JC, Turck M. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45(4): 493-496.
Braykov NP, Eisenberg JN, Grossman M, Zhang L, Vasco K, Cevallos W, Levy K. 2016. Antibiotic Resistance in Animal and Environmental Samples Associated with Small-Scale Poultry Farming in Northwestern Ecuador. mSphere. 1(1): e15.
Carter ME, Chengappa MM. 1990. Diagnostic Procedure in Veterinary Bacteriology and Mycology (G. R. Carter & J. R. Cole Eds. 5th Ed.): Academic Press.
De Carli S, Ikuta N, Lehmann FK, da Silveira,VP, de Melo PG, Fonseca AS, Lunge VR. 2015. Virulence gene content in Escherichia coli isolates from poultry flocks with clinical signs of colibacillosis in Brazil. Poult. Sci. 94(11): 2635-2640.
Jiang YW, Sims MD, Conway DP. 2005. The efficacy of TAMUS 2032 in preventing a natural outbreak of colibacillosis in broiler chickens in floor pens. Poult. Sci. 84(12): 1857-1859.
Joshi S, Singh R, Singh SP. 2012. Antibiotic resistance profile of Escherichia coli isolates from Colibacillosis in and around Pantnagar, India. Vet. World. 5(7): 405-408.
Luhung YGA, Suarjana IGK, Gelgel KTP. 2017. Sensitivitas Isolat Escherichia coli Patogen dari Organ Ayam Pedaging Terinfeksi Koliseptikemia terhadap Oksitetrasiklin, Ampisilin dan Sulfametoksazol. Bul. Vet. Udayana. 9(1): 60-66.
Messaiuml CR, Boukhors KT, Khelef, Hamdi H. 2013. Antimicrobial susceptibility of Escherichia coli strains isolated from broiler chickens affected by colibacillosis in Setif. Af. J. Microbiol. Res. 7(21): 2668-2672.
Oluduro AO. 2012. Antibiotic-resistant commensal Escherichia coli in faecal droplets from bats and poultry in Nigeria. Vet. Ital. 48(3): 297-308.
Qabajah M, Awwad E, Ashhab Y. 2014. Molecular characterisation of Escherichia coli from dead broiler chickens with signs of colibacillosis and ready-to-market chicken meat in the West Bank. Br. Poult. Sci. 55(4): 442-451.
Sayad A, Ibrahim F, Mukim US, Cho J, Madou M, Thong KL. 2018. A microdevice for rapid, monoplex and colorimetric detection of foodborne pathogens using a centrifugal microfluidic platform. Biosens. Bioelectron. 100: 96-104.
Schwaiger K, Schmied EM, Bauer J. 2008. Comparative analysis of antibiotic resistance characteristics of Gram-negative bacteria isolated from laying hens and eggs in conventional and organic keeping systems in Bavaria, Germany. Zoonoses Pub. Health. 55(7): 331-341.
Zende RJ, Chavhan DM, Suryawanshi PR, Rai AK, Vaidya VM. 2013. PCR detection and serotyping of enterotoxigenic and shigatoxigenic Escherichia coli isolates obtained from chicken meat in Mumbai, India. Vet. World. 6(10): 770-773.
