THE EFFECT OF PROPOLIS ON HISTOPATHOLOGICAL OBSERVATION IN WHITE RAT’S KIDNEY GIVEN HIGH DOSE OF PARACETAMOL

  • Elti Febilani
  • I Ketut Berata
  • Samsuri Samsuri
  • I Made Merdana
  • Luh Made Sudimartini

Abstract

The purpose of this study is to determine the effect of propolis on kidney histopathological representation of white rats were given the toxic dose paracetamol. 25 male rats males were used in this study who were divided into 5 groups, control group (P0) without treatment, group 1 (P1) that was given paracetamol doses 250mg/kg, group 2 (P2), group 3 (P3), and group 4 (P4), given paracetamol dose of 250mg / kg and propolis added 0.25 ml (P2), 0.50 ml (P3), and 0.75 ml (P4). Paracetamol and propolis were given orally by mixing it into the water provided ad libitum for 10 days. Then performed a necropsy and kidney  were taken aseptically for making of histopathology preparations with hematoxylin eosin (HE) staining. The examined variables were fatty degeneration and necrosis in the proximal tubules of the kidney. Results indicate that fatty degeneration and necrosis were showed in treatment P1, while the entire treatment P2, P3, and P4 show the repair from the damage caused by the paracetamol. Treatment P4 gives the best results in reducing the effects of the paracetamol. Kruskal-Wallis showed a significant difference from fatty degeneration and necrosis of the tested group. From this study it can be concluded that the propolis with dose 0.75 ml giving better protection than 0.25 ml and 0.50 ml dose.

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Author Biographies

Elti Febilani

Veterinarian practitioners in Jakarta

I Ketut Berata

Laboratory of Veterinary Pathology, Udayana University

Samsuri Samsuri

Laboratory of Veterinary Pharmacology and Pharmacy, Udayana University

I Made Merdana

Laboratory of Veterinary Pharmacology and Pharmacy, Udayana University

Luh Made Sudimartini

Laboratory of Veterinary Pharmacology and Pharmacy, Udayana University

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Published
2017-02-02
How to Cite
FEBILANI, Elti et al. THE EFFECT OF PROPOLIS ON HISTOPATHOLOGICAL OBSERVATION IN WHITE RAT’S KIDNEY GIVEN HIGH DOSE OF PARACETAMOL. Buletin Veteriner Udayana, [S.l.], feb. 2017. ISSN 2477-2712. Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/28726>. Date accessed: 31 oct. 2020.
Section
Articles

Keywords

Kata kunci: Parasetamol; propolis; radikal bebas; antioksidan; ginjal

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