DEVELOPMENT OF ADENOVIRUS VECTORED VACCINE FOR AFRICAN SWINE FEVER VIRUS BASED ON NON-STRUCTURAL PROTEIN
Abstract
African swine fever (ASF) is a viral disease of swine which causes high mortality as well as causes high economic losses in domestic pig. This study describes the process to develop an adenovirus vectors ASF vaccine with A224L and A276R coding region and its immune response in mice. Synthetic DNA containing the A224L and A276R genes was amplified and cloned into the commercial plasmid pAdenoX with an infusion cloning kit. A total of 106 AdenoX-ASF-224L-276R viruses from transfected T293 cells were injected into six mice intra-muscularly, while the other six mice were given T293 cell extract and were treated as controls. Blood without anticoagulant was collected from the facial vein of mice at 1, 3, 5, and 7 weeks after injection. Antibodies against ASF were tested by ELISA using synthetic peptides of A224L and A276R as antigens. AdenoX-ASF_A276R_A224L recombinant was successfully generated and proven. Injection of recombinant AdenoX-ASF_A276R_A224L in mice can trigger an immune response at week 7 significantly higher (p=0.00) compared to control, while weeks 1, 3 and 5 are not different from control (p>0.05). Production of recombinant adenovirus in monolayer cells and virus quantification need to be improved. Booster testing needs to be done after the third week.
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References
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Banjara S, Caria S, Dixon LK, Hinds MG, Kvansakul M. 2017. Structural insight into African Swine Fever virus A179L-mediated inhibition of apoptosis. J. Virol. 91(6): e02228-16.
Berrow NS, Alderton D, Owens RJ. 2009. The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning. Methods. Mol. Biol. 498: 75-90.
Bird LE, Rada H, Flanagan J, Diprose JM, Gilbert RJ, Owens RJ. 2014. Application of in-fusion cloning for the parallel construction of E. coli expression vectors. Methods. Mol. Biol. 1116: 209-234.
Bisimwa PN, Ongus JR, Steinaa L, Bisimwa EB, Bochere E, Machuka EM, Entfellner JD, Okoth E, Pelle R. 2021. The first complete genome sequence of the African swine fever virus genotype X and serogroup 7 isolated in domestic pigs from the Democratic Republic of Congo. Virol. J. 18: 23.
Blome S, Franzke K, Beer M. 2020. African swine fever - A review of current knowledge. Virus Res. 287: 198099.
Bustos MJ, Nogal ML, Revilla Y, Carrascosa AL. 2002. Plaque assay for African swine fever virus on swine macrophages. Arch. Virol. 147: 1453-1459.
Chang J. 2021. Adenovirus vectors: Excellent tools for vaccine development. Immune Netw. 21: e6.
Correia S, Ventura S, Parkhouse RM. 2013. Identification and utility of innate immune system evasion mechanisms of ASFV. Virus Res. 173: 87-100.
Cwynar P, Stojkov J, Wlazlak K. 2019. African Swine Fever status in Europe. Viruses. 11(4): 310.
de la Guardia C, Rangel G, Villarreal A, Goodridge A, Fernandez PL, Lleonart R. 2021. Development of in-house, indirect ELISAs for the detection of SARS-CoV-2 spike protein-associated serology in COVID-19 patients in Panama. PLoS One. 16: e0257351.
de Leon P, Bustos MJ, Carrascosa AL. 2013. Laboratory methods to study African swine fever virus. Virus Res. 173: 168-179.
Dharmayanti NI, Sendow I, Ratnawati A, Settypalli TBK, Saepulloh M, Dundon WG, Nuradji H, Naletoski I, Cattoli G, Lamien CE. 2021. African swine fever in North Sumatra and West Java provinces in 2019 and 2020, Indonesia. Trans. Emerg. Dis. 68: 2890-2896.
Dixon LK, Chapman DA, Netherton CL, Upton C. 2013. African swine fever virus replication and genomics. Virus Res. 173: 3-14.
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Galindo I, Alonso C. 2017. African Swine Fever Virus: A review. Viruses. 9(5): 103.
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Kavsan VM, Iershov AV, Balynska OV. 2011. Immortalized cells and one oncogene in malignant transformation: old insights on new explanation. BMC Cell Biol. 12: 23.
Kennedy RB. 2022. Efficacy of an adenovirus type 5 vectored SARS-CoV-2 vaccine. Lancet 399: 212-213.
Kleiboeker SB, Burrage TG, Scoles GA, Fish D, Rock DL. 1998. African swine fever virus infection in the argasid host, Ornithodoros porcinus porcinus. J. Virol. 72: 1711-1724.
Li X, Li Y, Chen S, Wang J. 2020. Construction of stable infectious full-length and eGFP-tagged cDNA clones of Mirabilis crinkle mosaic virus via In-Fusion cloning. Virus Res. 286: 198039.
Lu G, Pan J, Zhang G. 2020. African swine fever virus in Asia: Its rapid spread and potential threat to unaffected countries. J. Infect. 80: 350-371.
Malm M, Saghaleyni R, Lundqvist M, Giudici M, Chotteau V, Field R, Varley, PG, Hatton D, Grassi L, Svensson T. 2020. Evolution from adherent to suspension: systems biology of HEK293 cell line development. Sci. Rep. 10: 18996.
OIE 2022. African Swine Fever (OIE).
Olesen AS, Lohse L, Dalgaard MD, Wozniakowski G, Belsham GJ, Botner A, Rasmussen TB. 2018. Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid. J. Virol. Methods. 261: 14-16.
Oura CA, Edwards L, Batten CA. 2013. Virological diagnosis of African swine fever--comparative study of available tests. Virus Res. 173: 150-158.
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Park J, Throop AL, LaBaer J. 2015. Site-specific recombinational cloning using gateway and in-fusion cloning schemes. Curr. Protoc. Mol. Biol. 110: 3.20.1–3.20.23: 1-36.
Penrith ML, Kivaria FM. 2022. One hundred years of African swine fever in Africa: Where have we been, where are we now, where are we going? Trans. Emerg. Dis. 69(5): e1179-e1200.
Reis AL, Netherton C, Dixon LK. 2017. Unraveling the armor of a killer: evasion of host defenses by African swine fever virus. J. Virol. 91: e02338-02316.
Sanchez-Vizcaino JM, Mur L, Martinez-Lopez B. 2012. African swine fever: an epidemiological update. Trans. Emerg. Dis. 59(Suppl 1): 27-35.
Sleight SC, Sauro HM. 2013. BioBrick assembly using the in-fusion PCR cloning kit. Methods. Mol. Biol. 1073: 19-30.
Smalla K, Jechalke S, Top EM. 2015. Plasmid detection, characterization, and ecology. Microbiol. Spectr. 3(1): 1-21.
Tuo D, Shen W, Yan P, Li X, Zhou P. 2015. Rapid construction of stable infectious full-length cDNA clone of papaya leaf distortion mosaic virus using in-fusion cloning. Viruses. 7: 6241-6250.
Velumani S, Ho HT, He F, Musthaq S, Prabakaran M, Kwang J. 2011. A novel peptide ELISA for universal detection of antibodies to human H5N1 influenza viruses. PloS One. 6: e20737-e20737.
Vergne T, Chen-Fu C, Li S, Cappelle J, Edwards J, Martin V, Pfeiffer DU, Fusheng G, Roger FL. 2017. Pig empire under infectious threat: risk of African swine fever introduction into the People's Republic of China. Vet. Rec. 181: 117.
Wu L, Yang B, Yuan X, Hong J, Peng M, Chen JL, Song Z. 2021. Regulation and Evasion of Host Immune Response by African Swine Fever Virus. Front. Microbiol. 12: 698001.
Yildirim S, Thompson MG, Jacobs AC, Zurawski DV, Kirkup BC. 2016. Evaluation of parameters for high efficiency transformation of acinetobacter baumannii. Sci. Rep. 6: 22110.
Zhou X, Li N, Luo Y, Liu Y, Miao F, Chen T, Zhang S, Cao P, Li X, Tian K. 2018. Emergence of African Swine Fever in China, 2018. Trans. Emerg. Dis. 65: 1482-1484.
Aydin S. 2015. A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA. Peptides. 72: 4-15.
Banjara S, Caria S, Dixon LK, Hinds MG, Kvansakul M. 2017. Structural insight into African Swine Fever virus A179L-mediated inhibition of apoptosis. J. Virol. 91(6): e02228-16.
Berrow NS, Alderton D, Owens RJ. 2009. The precise engineering of expression vectors using high-throughput In-Fusion PCR cloning. Methods. Mol. Biol. 498: 75-90.
Bird LE, Rada H, Flanagan J, Diprose JM, Gilbert RJ, Owens RJ. 2014. Application of in-fusion cloning for the parallel construction of E. coli expression vectors. Methods. Mol. Biol. 1116: 209-234.
Bisimwa PN, Ongus JR, Steinaa L, Bisimwa EB, Bochere E, Machuka EM, Entfellner JD, Okoth E, Pelle R. 2021. The first complete genome sequence of the African swine fever virus genotype X and serogroup 7 isolated in domestic pigs from the Democratic Republic of Congo. Virol. J. 18: 23.
Blome S, Franzke K, Beer M. 2020. African swine fever - A review of current knowledge. Virus Res. 287: 198099.
Bustos MJ, Nogal ML, Revilla Y, Carrascosa AL. 2002. Plaque assay for African swine fever virus on swine macrophages. Arch. Virol. 147: 1453-1459.
Chang J. 2021. Adenovirus vectors: Excellent tools for vaccine development. Immune Netw. 21: e6.
Correia S, Ventura S, Parkhouse RM. 2013. Identification and utility of innate immune system evasion mechanisms of ASFV. Virus Res. 173: 87-100.
Cwynar P, Stojkov J, Wlazlak K. 2019. African Swine Fever status in Europe. Viruses. 11(4): 310.
de la Guardia C, Rangel G, Villarreal A, Goodridge A, Fernandez PL, Lleonart R. 2021. Development of in-house, indirect ELISAs for the detection of SARS-CoV-2 spike protein-associated serology in COVID-19 patients in Panama. PLoS One. 16: e0257351.
de Leon P, Bustos MJ, Carrascosa AL. 2013. Laboratory methods to study African swine fever virus. Virus Res. 173: 168-179.
Dharmayanti NI, Sendow I, Ratnawati A, Settypalli TBK, Saepulloh M, Dundon WG, Nuradji H, Naletoski I, Cattoli G, Lamien CE. 2021. African swine fever in North Sumatra and West Java provinces in 2019 and 2020, Indonesia. Trans. Emerg. Dis. 68: 2890-2896.
Dixon LK, Chapman DA, Netherton CL, Upton C. 2013. African swine fever virus replication and genomics. Virus Res. 173: 3-14.
Dixon LK, Sun H, Roberts H. 2019. African swine fever. Antiviral Res.165: 34-41.
Galindo I, Alonso C. 2017. African Swine Fever Virus: A review. Viruses. 9(5): 103.
Gallardo MC, Reoyo AT, Fernandez-Pinero J, Iglesias I, Munoz MJ, Arias ML. 2015. African swine fever: a global view of the current challenge. Porcine Health Manag. 1: 21.
Gao Z, Shao JJ, Zhang GL, Ge SD, Chang YY, Xiao L, Chang HY. 2021. Development of an indirect ELISA to specifically detect antibodies against African swine fever virus: bioinformatics approaches. Virology J. 18: 97.
Graham FL, Smiley J, Russell WC, Nairn R. 1977. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36: 59-74.
Hakizimana JN, Ntirandekura JB, Yona C, Nyabongo L, Kamwendo G, Chulu JLC, Ntakirutimana D, Kamana O, Nauwynck H, Misinzo G. 2021a. Complete genome analysis of African swine fever virus responsible for outbreaks in domestic pigs in 2018 in Burundi and 2019 in Malawi. Trop. Anim. Health Prod. 53: 438-438.
Hakizimana JN, Yona C, Kamana O, Nauwynck H, Misinzo G. 2021b. African Swine Fever virus circulation between tanzania and neighboring countries: A systematic review and meta-analysis. Viruses. 13(2): 306.
Halperin SA, Ye L, MacKinnon-Cameron D, Smith B, Cahn PE, Ruiz-Palacios GM, Ikram A, Lanas F, Lourdes Guerrero M, Munoz Navarro SR. 2022. Final efficacy analysis, interim safety analysis, and immunogenicity of a single dose of recombinant novel coronavirus vaccine (adenovirus type 5 vector) in adults 18 years and older: an international, multicentre, randomised, double-blinded, placebo-controlled phase 3 trial. Lancet. 399: 237-248.
Hanahan D, Jessee J, Bloom FR. 1991. Plasmid transformation of Escherichia coli and other bacteria. Methods Enzymol. 204: 63-113.
Irwin CR, Farmer A, Willer DO, Evans DH. 2012. In-fusion(R) cloning with vaccinia virus DNA polymerase. Methods Mol. Biol. 890: 23-35.
Kavsan VM, Iershov AV, Balynska OV. 2011. Immortalized cells and one oncogene in malignant transformation: old insights on new explanation. BMC Cell Biol. 12: 23.
Kennedy RB. 2022. Efficacy of an adenovirus type 5 vectored SARS-CoV-2 vaccine. Lancet 399: 212-213.
Kleiboeker SB, Burrage TG, Scoles GA, Fish D, Rock DL. 1998. African swine fever virus infection in the argasid host, Ornithodoros porcinus porcinus. J. Virol. 72: 1711-1724.
Li X, Li Y, Chen S, Wang J. 2020. Construction of stable infectious full-length and eGFP-tagged cDNA clones of Mirabilis crinkle mosaic virus via In-Fusion cloning. Virus Res. 286: 198039.
Lu G, Pan J, Zhang G. 2020. African swine fever virus in Asia: Its rapid spread and potential threat to unaffected countries. J. Infect. 80: 350-371.
Malm M, Saghaleyni R, Lundqvist M, Giudici M, Chotteau V, Field R, Varley, PG, Hatton D, Grassi L, Svensson T. 2020. Evolution from adherent to suspension: systems biology of HEK293 cell line development. Sci. Rep. 10: 18996.
OIE 2022. African Swine Fever (OIE).
Olesen AS, Lohse L, Dalgaard MD, Wozniakowski G, Belsham GJ, Botner A, Rasmussen TB. 2018. Complete genome sequence of an African swine fever virus (ASFV POL/2015/Podlaskie) determined directly from pig erythrocyte-associated nucleic acid. J. Virol. Methods. 261: 14-16.
Oura CA, Edwards L, Batten CA. 2013. Virological diagnosis of African swine fever--comparative study of available tests. Virus Res. 173: 150-158.
Oura CAL, Arias M. 2021. African swine fever (infection with African swine fever virus) In: OIE (Ed.) Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2021. OIE, Geneva. Pp. 1-18.
Park J, Throop AL, LaBaer J. 2015. Site-specific recombinational cloning using gateway and in-fusion cloning schemes. Curr. Protoc. Mol. Biol. 110: 3.20.1–3.20.23: 1-36.
Penrith ML, Kivaria FM. 2022. One hundred years of African swine fever in Africa: Where have we been, where are we now, where are we going? Trans. Emerg. Dis. 69(5): e1179-e1200.
Reis AL, Netherton C, Dixon LK. 2017. Unraveling the armor of a killer: evasion of host defenses by African swine fever virus. J. Virol. 91: e02338-02316.
Sanchez-Vizcaino JM, Mur L, Martinez-Lopez B. 2012. African swine fever: an epidemiological update. Trans. Emerg. Dis. 59(Suppl 1): 27-35.
Sleight SC, Sauro HM. 2013. BioBrick assembly using the in-fusion PCR cloning kit. Methods. Mol. Biol. 1073: 19-30.
Smalla K, Jechalke S, Top EM. 2015. Plasmid detection, characterization, and ecology. Microbiol. Spectr. 3(1): 1-21.
Tuo D, Shen W, Yan P, Li X, Zhou P. 2015. Rapid construction of stable infectious full-length cDNA clone of papaya leaf distortion mosaic virus using in-fusion cloning. Viruses. 7: 6241-6250.
Velumani S, Ho HT, He F, Musthaq S, Prabakaran M, Kwang J. 2011. A novel peptide ELISA for universal detection of antibodies to human H5N1 influenza viruses. PloS One. 6: e20737-e20737.
Vergne T, Chen-Fu C, Li S, Cappelle J, Edwards J, Martin V, Pfeiffer DU, Fusheng G, Roger FL. 2017. Pig empire under infectious threat: risk of African swine fever introduction into the People's Republic of China. Vet. Rec. 181: 117.
Wu L, Yang B, Yuan X, Hong J, Peng M, Chen JL, Song Z. 2021. Regulation and Evasion of Host Immune Response by African Swine Fever Virus. Front. Microbiol. 12: 698001.
Yildirim S, Thompson MG, Jacobs AC, Zurawski DV, Kirkup BC. 2016. Evaluation of parameters for high efficiency transformation of acinetobacter baumannii. Sci. Rep. 6: 22110.
Zhou X, Li N, Luo Y, Liu Y, Miao F, Chen T, Zhang S, Cao P, Li X, Tian K. 2018. Emergence of African Swine Fever in China, 2018. Trans. Emerg. Dis. 65: 1482-1484.
Published
2023-05-14
How to Cite
KAMESWARI, Made Sumitha; MAHARDIKA, I Gusti Ngurah Kade; SUARDANA, Ida Bagus Kade.
DEVELOPMENT OF ADENOVIRUS VECTORED VACCINE FOR AFRICAN SWINE FEVER VIRUS BASED ON NON-STRUCTURAL PROTEIN.
Buletin Veteriner Udayana, [S.l.], p. 873-887, may 2023.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/100408>. Date accessed: 21 nov. 2024.
doi: https://doi.org/10.24843/bulvet.2023.v15.i05.p23.
Section
Articles
