Pre-Storage Leukoreduced Filtration (PLF) Decrease Soluble Human Leukocyte Antigen-I (sHLA-I) Level in Thrombocyte Concentrate Stored for Five Days

  • S. Herawati Department of Clinical Pathology Faculty of Medicine Udayana University, Bali-Indonesia
  • I. W. P. Sutirta-Yasa Department of Clinical Pathology Faculty of Medicine Udayana University, Bali-Indonesia
  • K. Suega Department of Internal Medicine Faculty of Medicine Udayana University, Bali-Indonesia
  • A. A. N. Subawa Department of Clinical Pathology Faculty of Medicine Udayana University, Bali-Indonesia

Abstract

Background: Thrombocyte concentrate is one of the important blood component to improve patient's clinical condition. In order to provide thrombocyte, concentrate with good therapeutic effect, the preparation process and storage condition should be maintained properly. One attempt to maintain good quality of thrombocyte concentrates is by doing Pre-storage Leukoreduced Filtration (PLF) method during preparation of thrombocyte concentrates. The purpose of this study was to determine the effect of PLF on sHLA-I level in thrombocyte concentrates stored for 1, 3 and 5 days. Methods: This is an experimental study with posttest only Control Group design, enrolling 34 thrombocyte concentrates and randomly assigned into PLF Group and Control Group. Results: It was obtained that PLF Group has lower mean sHLA-I level than Control Group stored for 1, 3 and 5 days and statistically significant. Mean sHLA-I level in PLF Group at day 1 is 2,75±0,82 µg/ml and Control Group is 3,66±0,87 µg/ml, which is statistically significant (p < 0,05). Mean sHLA-I level in PLF Group at day 3 is 3,07±0,75 µg/ml and Control Group is 4,05±1,29 µg/ml, which is statistically significant (p < 0,05). Mean sHLA-I level in PLF Group at day 5 is 3,81±0,97 µg/ml and Control Group is 5,61±3,26 µg/ml, which is statistically significant (p < 0,05). Conclusions: It can be concluded that PLF decrease sHLA-I level in thrombocyte concentrates stored for five days compared with Control Groups.

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Author Biographies

S. Herawati, Department of Clinical Pathology Faculty of Medicine Udayana University, Bali-Indonesia
Department of Clinical Pathology Faculty of Medicine Udayana University, Bali-Indonesia
I. W. P. Sutirta-Yasa, Department of Clinical Pathology Faculty of Medicine Udayana University, Bali-Indonesia
Department of Clinical Pathology Faculty of Medicine Udayana University, Bali-Indonesia
K. Suega, Department of Internal Medicine Faculty of Medicine Udayana University, Bali-Indonesia
Department of Internal Medicine Faculty of Medicine Udayana University, Bali-Indonesia
A. A. N. Subawa, Department of Clinical Pathology Faculty of Medicine Udayana University, Bali-Indonesia
Department of Clinical Pathology Faculty of Medicine Udayana University, Bali-Indonesia
Published
2016-04-26
How to Cite
HERAWATI, S. et al. Pre-Storage Leukoreduced Filtration (PLF) Decrease Soluble Human Leukocyte Antigen-I (sHLA-I) Level in Thrombocyte Concentrate Stored for Five Days. BALI MEDICAL JOURNAL, [S.l.], v. 5, n. 1, apr. 2016. ISSN 2302-2914. Available at: <https://ojs.unud.ac.id/index.php/bmj/article/view/21789>. Date accessed: 13 nov. 2024.
Section
Articles

Keywords

PLF, sHLA-I, thrombocyte concentrate