Eksplorasi metode kultur tiga-dimensi sel punca mesenkim: Suatu kajian pustaka
Abstract
Kultur tiga dimensi sel punca mesenkim mempunyai lingkungan physicochemical yang relevan secara in vivo. Metode ini mampu mengatasi keterbatasan pada kultur sel 2D dalam meniru morfologi dan fisiologi sel. Penelitian kajian pustaka ini mengeksplorasi berbagai metode kultur 3D, seperti scaffolds berbasis biomaterial, scaffolds berbasis polimer sintetis, metode tanpa berbasis scaffolds, dan kultur berbasis hidrogel yang dianalisa untuk mengevaluasi keunggulan, kekurangan, dan potensinya. Hasil kajian menunjukkan bahwa berbagai macam kultur tiga dimensi menghasilkan spheroid dengan potensi yang berbeda, namun mampu mendukung interaksi antar sel dan interaksi sel-matriks ekstraseluler, meningkatkan viabilitas, dan derivat metabolik sel punca mesenkim untuk digunakan dalam metode pengobatan terkini. Kultur tiga dimensi juga memberikan lingkungan yang stabil untuk pemeliharaan sel karena distribusi oksigen dan nutrisi yang tersebar secara merata sehingga tidak menyebabkan manfaat dari sel punca mesenkim hilang. Dapat disimpulkan bahwa metode kultur tiga dimensi pada sel punca mesenkim mampu mengatasi keterbatasan pada kultur secara dua dimensi karena memiliki laingkungan fisiologis yang mirip jaringan asli. Teknik kultur tiga dimensi dapat meningkatkan interaksi antar sel dan sel dengan matriks ekstraseluler untuk meningkatkan viabilitas, proliferasi, diferensiasi, dan derivat metabolik pada sel punca mesenkim.
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References
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Ghannam SB, Kim K, Grainger DW, Okano T. 2021. 3D Cell Sheet Structure Augments Mesenchymal Stem Cell Cytokine Production. Journal of Scientific Reports 11(8170): 1-11.
Gupta S, Sharma A, Verma RS. 2021. Mesenchymal stem cells for cardiac regeneration from diferentiation to cell delivery. Stem Cell Rev Rep. 17(5): 1666–1694.
Han Y, Yang J, Fang J, Zhou Y, Candi E, Wang J, Hua D, Shao C, Shi Y. 2022. The Secretion Profile of Mesenchymal Stem Cells and Potential Applications in Treating Human Diseases. Nature International Journal 7(92): 1-19.
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Hernández AE, Garciá E. 2021. Mesenchymal Stem Cell Therapy for Alzheimer’s Disease. Stem Cells International 21(1): 1-12.
Huang X, Huang Z, Gao W, He W, Li Y, Crawford R, Zhou Y, Xiao L, Xiao Y. 2022. Current Advances in 3D Dynamic Cell Culture Systems. Gels International Journal 8(289): 1-20.
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Islam MD, Ang S, Andriyana ABC, Afifi AM. 2019. A Review on Fabrication of Nanofbers via Electrospinning and Their Applications. Applied Sciences Jornal. 1(1248): 1-16.
Jang, E.H., Kim, J.H., Lee, J.H., Kim, D.H., Youn, Y.N. 2020. Enhanced Biocompatibility of Multi-Layered, 3D Bio-Printed Artifcial Vessels Composed of Autologous Mesenchymal Stem Cells. Polymers 12(538): 1-12.
Jensen C, Teng Y. 2020. Is It Time to Start Transitioning from 2D to 3D Cell Culture?. Journal of Molecular Biosciences 7(33): 1-15.
Jin Q, Jin G, Ju J, Xu L, Tang L, Fu Y, Hou R, Atala A, Zhao W. 2022. Bioprinting Small-Diameter Vascular Vessel with Endothelium and Smooth Muscle by The Approach of Two-Step Crosslinking Process. Biotechnology Bioengineering 119: 1673–1684.
Kapałczyńska M, Kolenda T, Przybyla W, Zajączkowska M, Teresiak A, Filas V, Ibbs M, Bliźniak R, Łuczewski L, Lamperska K. 2016. 2D and 3D Cell Cultures – A Comparison of Different Types of Cancer Cell Cultures. Archives of Medical Science 14(4): 910-919.
Kim H, Choi S, Kim H. 2013. Mesenchymal Stem Cell-Derived Secretome and Microvesicles as a Cell-Free Therapeutics for Neurodegenerative Disorders. Tissue Engineering and Regenerative Medicine 10(3): 93-101.
Kim Y, Kim I, Shin K. 2023. A New Era of Stem Cell and Developmental Biology: From Blastoids to Synthetic Embryos and Beyond. Korean Society for Biochemistry and Molecular Biology 55: 2127-2137.
Langhans SA. 2018. Three-Dimensional in Vitro Cell Culture Models in Drug Discovery and Drug Repositioning. Frontiers in Pharmacology 9(6): 1-14.
Li J, Yao Y, Zhou J, Yang Z, Qiu C, Lu Y, Xie J, Liu J, Jiang T, Kou Y, Ge Z, Liang P, Qiu C, Shen L, Zhu Y, Gao C, Yu L. 2024. Epicardial Transplantation of Antioxidant Polyurethane Scaffold Based Human Amniotic Epithelial Stem Cell Patch for Myocardial Infarction Treatment. Nature Communications 15(9105): 1-16.
Li T, Xia M, Gao Y, Chen Y, Xu Y. 2015. Human Umbilical Cord Mesenchymal Stem Cells: An Overview of Their Potential in Cell-Based Therapy. Expert Opinion on Biological Therapy 15(9): 1293-1306.
Liu F, Huang J, Ning B, Liu Z, Chen S, Zhao W. 2016. Drug Discovery Via Human-Derived Stem Cell Organoids. Frontiers in Pharmacology 7(334): 1-12.
Ma ZJ, Yang JJ, Lu YB, Liu AY, Wang XX. 2020. Mesenchymal Stem Cell-Derived Exosomes: Toward Cell-Free Therapeutic Strategies in Regenerative Medicine. World Journal Stem Cells 12(8): 814-840.
Marei NH, Sherbiny ME, Lotfy A, Badawy AE. 2016. Mesenchymal Stem Cells Growth and Proliferation Enhancement Using PLA vs PCL Based Nanofibrous Scaffolds. International Journal of Biological Macromolecules 93: 9-19.
McNamara MC, Sharifi F, Wrede AH, Kimlinger DF, Thomas DG, Wiel JBV, Chen Y, Montazami R, Hashemi NN. 2017. Microfibers as Physiologically Relevant Platforms for Creation of 3D Cell Cultures. Macromolecular Bioscience Journal 1700279: 1-18.
Milovac D, Ferrer GG, Ivankovic M, Ivankovic H. 2014. PCL-Coated Hydroxyapatite Scaffold Derived from Cuttlefish Bone: Morphology, Mechanical Properties, and Bioactivity. Materials Science and Engineering C 34: 437–445.
Muzes G, Sipos F. 2022. Mesenchymal Stem Cell-Derived Secretome: A Potential Therapeutic Option for Autoimmune and Immune-Mediated Inflammatory Diseases. Cells Journal 11(15): 1-23.
Nedjari S, Awaja F, Altankov G. 2017. Three-Dimensional Honeycomb Patterned Fibrinogen Based Nanofbers Induce Substantial Osteogenic Response of Mesenchymal Stem Cells. Scientific Reports 7(15947): 1-11.
Nurhayati RW, Lubis DSH, Pratama G, Agustina E, Khoiriyah Z, Alawiyah K, Pawitan JA, 2021. The Effects of Static and Dynamic Culture Systems on Cell Proliferation and Conditioned Media of Umbilical Cord-Derived Mesenchymal Stem Cells. International Journal of Technology 12(6): 1187-1197.
Patil S, Singh N. 2019. Silk Fibroin-Alginate Based Beads for Human Mesenchymal Stem Cell Differentiation in 3D. Biomaterial Science 5(11): 1-28.
Pedersen DD, Kim S, Wagner WR. 2022. Biodegradable Polyurethane Scaffolds in Regenerative Medicine: Clinical Translation Review. Journal of Biomedical Materials Research 110(8): 1460-1487.
Peshkova M, Korneev A, Suleimanov S, Vlasova II, Svistunov A, Kosheleva N, Timashev P. 2023. MSCs Conditioned Media Cytokine and Growth Factor Profles and Their Impact on Macrophage Polarization. Stem Cell Research and Therapy Journal 14(142): 1-15.
Pittenger MF, Discher DE, Péault BM, Phinney DG, Hare JM, Caplan AI. 2019. Mesenchymal Stem Cell Perspective: Cell Biology to Clinical Progress. Regenerative Medicine Nature Partner Journals 22: 1-15.
Prats RG, Rimblas MC, Puig F, Herrero R, Tantinya N, Mollar AS, Artigas A. 2020. Alveolar Type II Cells or Mesenchymal Stem Cells: Comparison of Two Different Cell Therapies for the Treatment of Acute Lung Injury in Rats. Cells International Journal 9(8): 1-15
Rofaani E, Mardani MW, Yutiana PN, Amanda O, Darmawan N. 2024. Differentiation of Mesenchymal Stem Cells Into Vascular Endothelial Cells In 3D Culture: A Mini Review. Molecular Biology Reports 51(781). 1-12.
Rogers CJ, Harman RJ, Bunnell BA, Schreiber MA, Xiang C, Wang FS, Santidrian AF, Minev BR. 2020. Rationale for The Clinical Use of Adipose-Derived Mesenchymal Stem Cells for COVID-19 Patients. Journal of Translational Medicine 18(203): 1-19.
Ryu NE, Lee SH, Park H. 2019. Spheroid Culture System Methods and Applications for Mesenchymal Stem Cells. Cells 8(12): 1-13.
Ruiz AC, Mendonca FE, Otero RM, Resende VTR. 2015. Biological Behavior of Mesenchymal Stem Cells on Poly-Ε-Caprolactone Filaments and A Strategy for Tissue Engineering of Segments of The Peripheral Nerves. Stem Cell Research and Theraphy 6(128): 1-15.
Scalise M, Marino F, Salerno L, Cianflone E, Molinaro C, Salerno N, Angelis AD, Viglietto G, Urbanek K, Torella D. 2021. From Spheroids to Organoids: The Next Generation of Model Systems of Human Cardiac Regeneration in a Dish. International Journal of Molecular Sciences 22(13180): 1-25.
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Published
2025-06-10
How to Cite
MELIAWATI, Widia; WIRATMINI, Ngurah Intan; ROFAANI, Elrade.
Eksplorasi metode kultur tiga-dimensi sel punca mesenkim: Suatu kajian pustaka.
Jurnal Biologi Udayana, [S.l.], v. 29, n. 1, p. 10-19, june 2025.
ISSN 2599-2856.
Available at: <https://ojs.unud.ac.id/index.php/bio/article/view/120893>. Date accessed: 31 july 2025.
doi: https://doi.org/10.24843/JBIOUNUD.2025.v29.i01.p02.
Section
Articles
