The Variation of antioxidant activity profile of Dendrophthoe pentandra (L.) Miq. leaves extract from two varieties of mangoes (Mangifera indica)
Abstract
Mistletoe is a hemiparasites plant that interfere the growth of their host plant, However, mistletoe itself can be used in the herbal medicine. One of the cultivated plants is Indonesia which is affected by this mistletoe is mango. Mangoes is rich in antioxidant, so that its mistletoe is also thought to rich in antioxidant. This study is aimed to analyze the secondary metabolites and antioxidant activity of mango mistletoe from two varieties of mangoes, namely Harumanis and Madu. The study has been carried out experimentally. Samples were collected from Caturtunggal, Sleman, Yogyakarta. Extraction was carried by maceration in methanol. Phytochemical screening, quantitative analysis of phenolics compound and quercetin were performed using HPLC. Antioxidant activity was determined using DPPH method. Data was analyzed through descriptive statistisc. The result showed that the quercetin level and total phenolic compound of D.pentandra leaves extract from M.indica var. Madu are higher than var. Harumanis, which are 257.917 ppm and 399,195 ± 12.352 mg GAE/g extract respectively. Antioxidant activity of D.pentandra extract from M.indica var. Madu is also higher than var. Harumanis, with IC50 37.19 ppm. In this study, enviromental factor did not affect the secondary metabolites of mistletoe. Through this data, the difference of secondary metabolites profile and the antioxidant activity of mistletoe from different host varieties were found, particularly in quercetin and its phenolic compounds.
Downloads
Download data is not yet available.
References
Alharits L, Handayani W, Yasman, Hemelda NM. 2019. Phytochemical analysis and antioxidant activity of leaves and flowers extracts of mistletoe (Dendrophthoe pentandra (L.) Miq.), collected from UI Campus, Depok. AIP Conference Proceedings 2168. https://doi.org/10.1063/1.5132528
Artanti N, Firmansyah T, Darmawan A. 2012. Bioactivities evaluation of indonesian mistletoes (Dendrophthoe pentandra (L.) Miq.) leaves extracts. Journal of Applied Pharmaceutical Science 2(1): 24–27.
Assanga SBI, Luján LML, Ruiz JCG, McCarty MF, Cota-Arce JM, Espinoza CLL, Salido AAG, Ángulo DF. 2020. Comparative analysis of phenolic content and antioxidant power between parasitic Phoradendron californicum (toji) and their hosts from Sonoran Desert. Results in Chemistry 2: 100079. https://doi.org/10.1016/j.rechem.2020.100079
Badarinath AV, Rao KM, Chetty CM, Ramkanth STVSR, Rajan TVS, Gnanaprakash K. 2010. A Review on In-Vitro Antioxidant Methods; Comparisions, Correlations and Considerations. International Journal of PharmTech Research 2(2): 1276-1285.
Dewi RT, Ekapratiwi Y, Sundowo A, Ariani N, Yolanda T, Filaila E. 2019. Bioconversion of quercetin glucosides from Dendrophthoe pentandra leaf using Aspergillus acueletus LS04-3. AIP Conference Proceedings 2175. https://doi.org/10.1063/1.5134612
Elsyana V, Bintang M, Priosoeryanto BP. 2016. Cytotoxicity and antiproliferative activity assay of clove mistletoe (Dendrophthoe pentandra (L.) Miq.) leaves extracts. Advances in Pharmacological Sciences 2016: 3242698. https://doi.org/10.1155/2016/3242698
Harborne JB. 1984. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis (2nd ed.). Chapman and Hall.
Hardiyanti R, Marpaung L, Adnyana IK, Simanjuntak P. 2019. Isolation of quercitrin from Dendrophthoe pentandra (L.) Miq leaves and its antioxidant and antibacterial activities. Rasayan Journal of Chemistry 12(4): 1822–1827. https://doi.org/10.31788/RJC.2019.1235353
Hosseini SM, Kartoolinejad D, Mirnia SK. 2008. The European Mistletoe Effects on Leaves and Nutritional Elements of Two Host Species in Hyrcanian Forests. Silva Lusitana 16(2): 229–237.
Isah T. 2019. Stress and Defense Responses in Plant Secondary Metabolites Production. Biological Research 52(1): 1–25. https://doi.org/10.1186/s40659-019-0246-3
Kumar S, Abedin MdM, Singh AK, Das S. 2020. Role of Phenolic Compounds in Plant-Defensive Mechanisms. In Plant Phenolics in Sustainable Agriculture (pp. 517–532). Springer Singapore. https://doi.org/10.1007/978-981-15-4890-1_22
Kurniasih N, Kusmiyati M, Nurhasanah, Puspita Sari R, Wafdan R. 2015. Potensi Daun Sirsak (Annona muricata Linn), Daun Binahong (Anredera cordifolia (Ten) Steenis), Dan Daun Benalu Mangga (Dendrophthoe pentandra) Sebagai Antioksidan Pencegah Kanker. Jurnal Istek 9(1): 162–184.
Moghadamtousi SZ, Kamarudin MNA, Chan CK, Goh BH, Kadir HA. 2014. Phytochemistry and biology of Loranthus parasiticus Merr., a commonly used herbal medicine. American Journal of Chinese Medicine 42(1): 23–35. https://doi.org/10.1142/S0192415X14500025
Noratto GD, Bertoldi MC, Krenek K, Talcott ST, Stringheta PC, Mertens-Talcott SU. 2010. Anticarcinogenic effects of polyphenolics from mango (Mangifera indica) varieties. Journal of Agricultural and Food Chemistry 58(7): 4104–4112. https://doi.org/10.1021/jf903161g
Özcan MM, Doğu S, Uslu N. 2018. Effect of species on total phenol, antioxidant activity and phenolic compounds of different wild onion bulbs. Journal of Food Measurement and Characterization 12(2): 902–905. https://doi.org/10.1007/s11694-017-9705-0
Yulianti R, R., Dahlia, A. A., & Ahmad, A. R. (2016). Penetapan Kadar Flavonoid Total dari Ekstrak Etanolik Daun Benalu Mangga (Dendrophthoe pentandra L. Miq). Jurnal Fitofarmaka Indonesia, 1(1), 14–17. https://doi.org/10.33096/jffi.v1i1.1.95
Saptarini NM, Herawati IE, Permatasari UY. 2016. Total flavonoids content in acidified extract of flowers and leaves of gardenia (Gardenia jasminoides Ellis). Asian Journal of Pharmaceutical and Clinical Research 9(1): 213–215.
Skorek M, Jurczyk K, Sajewicz M, Kowalska T. 2016. Thin-layer chromatographic identification of flavonoids and phenolic acids contained in cosmetic raw materials. Journal of Liquid Chromatography and Related Technologies 39(5–6): 286–291. https://doi.org/10.1080/10826076.2016.1163467
Szurpnicka A, Kowalczuk A, Szterk A. 2020. Biological activity of mistletoe: in vitro and in vivo studies and mechanisms of action. In Archives of Pharmacal Research 43(6): 593–629). Pharmaceutical Society of Korea. https://doi.org/10.1007/s12272-020-01247-w
Teixeira-Costa L. 2021. A living bridge between two enemies: haustorium structure and evolution across parasitic flowering plants. Revista Brasileira de Botanica 44(1): 165–178. https://doi.org/10.1007/s40415-021-00704-0
Vicaş SI, Ruginǎ D, Socaciu C. 2011. Comparative study about antioxidant activities of viscum album from different host trees, harvested in different seasons. Journal of Medicinal Plants Research 5(11): 2237–2244.
Zagorchev L, Stöggl W, Teofanova D, Li J, Kranner I. 2021. Plant parasites under pressure: Effects of abiotic stress on the interactions between parasitic plants and their hosts. International Journal of Molecular Sciences 22(14): 22147418. https://doi.org/10.3390/ijms22147418
Zeb A. 2020. Concept, Mechanism, and Applications of Phenolic Antioxidants in Foods. Journal of Food Biochemistry 44(9): 1–22. https://doi.org/10.1111/jfbc.13394
Zhang Q, Zhang J, Shen J, Silva A, Dennis DA, Barrow CJ. 2006. A simple 96-well microplate method for estimation of total polyphenol content in seaweeds. Journal of Applied Phycology 18(3–5): 445–450. https://doi.org/10.1007/s10811-006-9048-4
Artanti N, Firmansyah T, Darmawan A. 2012. Bioactivities evaluation of indonesian mistletoes (Dendrophthoe pentandra (L.) Miq.) leaves extracts. Journal of Applied Pharmaceutical Science 2(1): 24–27.
Assanga SBI, Luján LML, Ruiz JCG, McCarty MF, Cota-Arce JM, Espinoza CLL, Salido AAG, Ángulo DF. 2020. Comparative analysis of phenolic content and antioxidant power between parasitic Phoradendron californicum (toji) and their hosts from Sonoran Desert. Results in Chemistry 2: 100079. https://doi.org/10.1016/j.rechem.2020.100079
Badarinath AV, Rao KM, Chetty CM, Ramkanth STVSR, Rajan TVS, Gnanaprakash K. 2010. A Review on In-Vitro Antioxidant Methods; Comparisions, Correlations and Considerations. International Journal of PharmTech Research 2(2): 1276-1285.
Dewi RT, Ekapratiwi Y, Sundowo A, Ariani N, Yolanda T, Filaila E. 2019. Bioconversion of quercetin glucosides from Dendrophthoe pentandra leaf using Aspergillus acueletus LS04-3. AIP Conference Proceedings 2175. https://doi.org/10.1063/1.5134612
Elsyana V, Bintang M, Priosoeryanto BP. 2016. Cytotoxicity and antiproliferative activity assay of clove mistletoe (Dendrophthoe pentandra (L.) Miq.) leaves extracts. Advances in Pharmacological Sciences 2016: 3242698. https://doi.org/10.1155/2016/3242698
Harborne JB. 1984. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis (2nd ed.). Chapman and Hall.
Hardiyanti R, Marpaung L, Adnyana IK, Simanjuntak P. 2019. Isolation of quercitrin from Dendrophthoe pentandra (L.) Miq leaves and its antioxidant and antibacterial activities. Rasayan Journal of Chemistry 12(4): 1822–1827. https://doi.org/10.31788/RJC.2019.1235353
Hosseini SM, Kartoolinejad D, Mirnia SK. 2008. The European Mistletoe Effects on Leaves and Nutritional Elements of Two Host Species in Hyrcanian Forests. Silva Lusitana 16(2): 229–237.
Isah T. 2019. Stress and Defense Responses in Plant Secondary Metabolites Production. Biological Research 52(1): 1–25. https://doi.org/10.1186/s40659-019-0246-3
Kumar S, Abedin MdM, Singh AK, Das S. 2020. Role of Phenolic Compounds in Plant-Defensive Mechanisms. In Plant Phenolics in Sustainable Agriculture (pp. 517–532). Springer Singapore. https://doi.org/10.1007/978-981-15-4890-1_22
Kurniasih N, Kusmiyati M, Nurhasanah, Puspita Sari R, Wafdan R. 2015. Potensi Daun Sirsak (Annona muricata Linn), Daun Binahong (Anredera cordifolia (Ten) Steenis), Dan Daun Benalu Mangga (Dendrophthoe pentandra) Sebagai Antioksidan Pencegah Kanker. Jurnal Istek 9(1): 162–184.
Moghadamtousi SZ, Kamarudin MNA, Chan CK, Goh BH, Kadir HA. 2014. Phytochemistry and biology of Loranthus parasiticus Merr., a commonly used herbal medicine. American Journal of Chinese Medicine 42(1): 23–35. https://doi.org/10.1142/S0192415X14500025
Noratto GD, Bertoldi MC, Krenek K, Talcott ST, Stringheta PC, Mertens-Talcott SU. 2010. Anticarcinogenic effects of polyphenolics from mango (Mangifera indica) varieties. Journal of Agricultural and Food Chemistry 58(7): 4104–4112. https://doi.org/10.1021/jf903161g
Özcan MM, Doğu S, Uslu N. 2018. Effect of species on total phenol, antioxidant activity and phenolic compounds of different wild onion bulbs. Journal of Food Measurement and Characterization 12(2): 902–905. https://doi.org/10.1007/s11694-017-9705-0
Yulianti R, R., Dahlia, A. A., & Ahmad, A. R. (2016). Penetapan Kadar Flavonoid Total dari Ekstrak Etanolik Daun Benalu Mangga (Dendrophthoe pentandra L. Miq). Jurnal Fitofarmaka Indonesia, 1(1), 14–17. https://doi.org/10.33096/jffi.v1i1.1.95
Saptarini NM, Herawati IE, Permatasari UY. 2016. Total flavonoids content in acidified extract of flowers and leaves of gardenia (Gardenia jasminoides Ellis). Asian Journal of Pharmaceutical and Clinical Research 9(1): 213–215.
Skorek M, Jurczyk K, Sajewicz M, Kowalska T. 2016. Thin-layer chromatographic identification of flavonoids and phenolic acids contained in cosmetic raw materials. Journal of Liquid Chromatography and Related Technologies 39(5–6): 286–291. https://doi.org/10.1080/10826076.2016.1163467
Szurpnicka A, Kowalczuk A, Szterk A. 2020. Biological activity of mistletoe: in vitro and in vivo studies and mechanisms of action. In Archives of Pharmacal Research 43(6): 593–629). Pharmaceutical Society of Korea. https://doi.org/10.1007/s12272-020-01247-w
Teixeira-Costa L. 2021. A living bridge between two enemies: haustorium structure and evolution across parasitic flowering plants. Revista Brasileira de Botanica 44(1): 165–178. https://doi.org/10.1007/s40415-021-00704-0
Vicaş SI, Ruginǎ D, Socaciu C. 2011. Comparative study about antioxidant activities of viscum album from different host trees, harvested in different seasons. Journal of Medicinal Plants Research 5(11): 2237–2244.
Zagorchev L, Stöggl W, Teofanova D, Li J, Kranner I. 2021. Plant parasites under pressure: Effects of abiotic stress on the interactions between parasitic plants and their hosts. International Journal of Molecular Sciences 22(14): 22147418. https://doi.org/10.3390/ijms22147418
Zeb A. 2020. Concept, Mechanism, and Applications of Phenolic Antioxidants in Foods. Journal of Food Biochemistry 44(9): 1–22. https://doi.org/10.1111/jfbc.13394
Zhang Q, Zhang J, Shen J, Silva A, Dennis DA, Barrow CJ. 2006. A simple 96-well microplate method for estimation of total polyphenol content in seaweeds. Journal of Applied Phycology 18(3–5): 445–450. https://doi.org/10.1007/s10811-006-9048-4
Published
2025-01-06
How to Cite
ADITIYARINI, Dwi; ELSA, Yohana; RESTIANI, Ratih.
The Variation of antioxidant activity profile of Dendrophthoe pentandra (L.) Miq. leaves extract from two varieties of mangoes (Mangifera indica).
Jurnal Biologi Udayana, [S.l.], v. 28, n. 2, p. 328-337, jan. 2025.
ISSN 2599-2856.
Available at: <https://ojs.unud.ac.id/index.php/bio/article/view/112661>. Date accessed: 08 jan. 2025.
doi: https://doi.org/10.24843/JBIOUNUD.2024.v28.i02.p13.
Section
Articles
