Effects of black soya extract (Glycine soja) on histology femur of mice (Mus musculus) ovariectomized

  • mahhriani mahriani Jurusan Biologi, Fakultas Matematika dan IlmuPengetahuan Alam,Universitas Jember
  • Resa Miftahatu Yuniar Jurusan Biologi, Fakultas Matematika dan IlmuPengetahuan Alam,Universitas Jember
  • Masrurotul Hasanah Jurusan Biologi, Fakultas Matematika dan IlmuPengetahuan Alam,Universitas Jember
  • Eva Tyas Utami Jurusan Biologi, Fakultas Matematika dan IlmuPengetahuan Alam,Universitas Jember

Abstract

Bone is a dynamic structure and has the process to remodelling or regeneration. Bone remodelling includes the process of formation by osteoblasts and the process of resorption by osteoclasts. The balancing between formation and resorption are assisted by the estrogen hormone.Estrogen deficiency can lead the bone resorption. Ovariectomy is a model for estrogen deficiency by removing the ovaries. One way to overcome estrogen deficiency can be solved by offering phytoestrogens. This research have purpose to determine the effect of black soybean extract (Glycine soja) on the histology of the femur mice (Mus musculus) ovariectomized. In this research used a dose of black soybean extract as many as 0.42 g /ml/day and 0.63 g/ml/day, which are given orally for 30 days. The research is conducted with a completele randomized design with 4 treatments and 6 replications. Observations are made on the number of osteoblasts and the number of osteoclasts on the histological of the femur. The results showed that offering black soybean extract at a dose of 0.42 g/ml/day for 30 days have significantly increased the number of osteoblasts and have decreased the number of osteoclasts in the femur of ovariectomized mice.

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References

Alagwu EA, Nneli RO. 2005. Effect of Ovariectomy on The Levels of Plasma Sex Hormones In Albino Rats. Nigerian Journal of Physiological Sciences 20(1-2): 90-94.
Bell NH. 2003. RANK Ligand and The Regulation of Skeletal Remodeling. J Clin Invest. 111(8): 1120-1122.
Bilbao NJL, Sanchez CA, Gil-Antunano SP. 2011. Bone Metabolism Regulation Through RANK-RANKL-OPG System. Rev Osteoporos Metab Miner 3(2): 105-112
Brune B, Knethen AV, Sandau KB. 1999. Nitric oxide (NO): an effector of apoptosis. Cell Death and Differentation 6: 969-975.
Chen MN, Lin C-C, Liu C. 2015. Efficacy of phytoestrogens for menopausal symptoms: a meta-analysis and systemic review. Climateric 18(2):1-20.
Darmadi D, Nurdiana, Norahmawati E. 2011. Efek Ekstrak Kacang Tunggak Terhadap Osteoblas dan Osteoklas pada Tikus dengan Ovariektomi. Jurnal Kedokteran Brawijaya 26(3): 15:1-155.
Khosla S, Oursler MJ, Monroe DG. 2012. Estrogen and the skeleton. Trends in Endocrinology and Metabolism 23(11): 576-581.
Kini U, Nandeesh BN. 2012. Physiology of Bone Formation, Remodeling, and Metabolism. Springer-Verley: Berlin.
Kuiper GGJM, Lemmen JG, Carlsson BO, Corton JC, Safe SH, Saag PTDV, Burg BVD, Gustafsson J. 1998. Interaction of Estrogenic eceptor B. Endocrinology 139(10): 4252-4263
Mann GE, Rowlands DJ, Li FYL, Winter P, Siow RCM. 2007. Activation of endothelial nitric oxide synthase by dietary isoflavones: Role of NO in Nrf2-mediated antioxidant gene expression. Cardiovascular Research 75: 261–274.
Masyitha D. 2006. Struktur Mikroskopik tulang Mandibula Pada Tikus Ovariektomi dan Pemberian Pekan Rasio Fosfat/kalsium Tinggi. Media Kedokteran Hewan 22(2): 112-117.
Mescher AL. 2012. Histologi Dasar Junqueira Teks &Atlas. EGC: Jakarta.
Nebel D, Bratthall G, Warfvinge G, Nilsson BO. 2009. Effect of Ovariectomy and Aging on Tooth Attachment in Female Mice Assessed by Morphometric Anaysis. Acta Odontologica Scandinavica. 67: 8-12.
Nurrahman. 2015. Evaluasi komposisi zat gizi dan senyawa antioksidan kedelai hitam dan kedelai kuning. Jurnal Aplikasi Teknologi Pangan 4(3): 89-93.
Sabri M. 2011. Aktivitas Ekstrak Etanol Batang Sipatah-patah (Cissus quadrangula Salibs) sebagai Antiosteoporosis pada Tikus (Rattus norvegicus). Disertasi. Sekolah Pasca Sarjana Institut Pertanian Bogor.
Seibel M. 2005. Biochemical Markers of Bone turnover part 1: Biochemistry and variability. Clin Biochem Rev. 26: 97-122.
Steel R, Torrie J. 1993. Principles and Procedures of Statistics. Gramedia Pustaka: Jakarta.
Suarsana IN, Silitonga SL, Dharmawan INS, Kardenadan IM, Priosuryanto BP. 2014. Pemberian Tepung Tempe Meningkatkan Kualitas Tulang Pada Tikus Ovariektomi.Jurnal Veteriner 15(4): 548-556.
Sumaryati. 2012. Dekalsifikasi Metode Von Ebner’s.Prosedur Kerja Baku/Standart Operating Procedure (SOP). No: P-014. Yogyakarta: Laboratorium Histologi dan Biologi Sel Fakultas Kedokteran, Universitas Gadjah Mada.
Suntoro SH. 1983. Metode Pewarnaan. Bantara Karya Aksara: Jakarta.
Tortora G, Derrickson B .2014. Principles of Anatomy and Physiology. John Wiley & Sons, Inc.: Hoboken.
Ulfa YM. 2017. Efek Ekstrak Tepung Kedelai Hitam (Glycine soja) terhadap Struktur Histologi Uterus Mencit (Mus musculus) Strain Swiss Webster Ovariektomi Unilateral. Skripsi. Universitas Jember.
Wang X, Chen S, Ma G, Ye M, Lu G. 2005. Genestein protects dopaminegic neuron by inhibiting microgial avtivation. Neuroreport 16(3): 267-270
Wiyasa IWA, Norahmawati E, Soehartono. 2008. Pengaruh isoflavone genistein dan daidzein ekstrak tokbi (Peuraria lobata) strain Kangean terhadap jumlah osteoblas dan osteoklas (Rattus novergicus) Wistar hipoestrogenik. Indonesian Journal of Obstetrics and gynecology 32(3): 148-152.
Yamaguchi M. 2002. Isoflavone and Bone metabolism:its cellular mechanism and preventive role in bone loss. Journal of Health science 48(3): 209-222.
Published
2021-06-25
How to Cite
MAHRIANI, mahhriani et al. Effects of black soya extract (Glycine soja) on histology femur of mice (Mus musculus) ovariectomized. Jurnal Biologi Udayana, [S.l.], v. 25, n. 1, p. 39-45, june 2021. ISSN 2599-2856. Available at: <https://ojs.unud.ac.id/index.php/bio/article/view/68392>. Date accessed: 29 mar. 2024. doi: https://doi.org/10.24843/JBIOUNUD.2021.v25.i01.p05.