Biokompatibilitas scaffold sutera asal Bombyx mori ukuran pori 100µm terhadap adipose-derived stem cells (ADSCs) yang dikultur pada berbagai medium pertumbuhan

  • Imam Rosadi HayandraLab, Yayasan Hayandra Peduli, Jakarta, Indonesia
  • Karina Karina HayandraLab, Yayasan Hayandra Peduli, Jakarta, Indonesia; Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Biomedik, Universitas Indonesia, Jakarta, Indonesia
  • Komang A. Wahyuningsih HayandraLab, Yayasan Hayandra Peduli, Jakarta, Indonesia; Klinik Hayandra, Yayasan Hayandra Peduli, Jakarta, Indonesia; Histologi, Universitas Katolik Indonesia Atma Jaya, Jakarta, Indonesia
  • Anggraini Barlian Sekolah Ilmu dan Teknologi Hayati, Institut Teknologi Bandung, Bandung, Indonesia
  • Iis Rosliana HayandraLab, Yayasan Hayandra Peduli, Jakarta, Indonesia
  • Tias Widyastuti HayandraLab, Yayasan Hayandra Peduli, Jakarta, Indonesia
  • Siti Sobariah HayandraLab, Yayasan Hayandra Peduli, Jakarta, Indonesia
  • Irsyah Afini HayandraLab, Yayasan Hayandra Peduli, Jakarta, Indonesia

Abstract

Rekayasa jaringan terdiri dari 3 komponen utama yaitu sel, nutrisi, dan scaffold. Penggunaan sel punca asal jaringan lemak (adipose-derived stem cells/ ADSCs) telah banyak dikembangkan sebagai sumber sel dalam teknologi rekayasa jaringan. Medium yang digunakan dalam mendukung pertumbuhan sel diantaranya medium yang mengandung serum seperti fetal bovine serum (FBS), kombinasi FBS dan L-ascorbic acid 2-phosphate (LAA) atau platelet-rich plasma (PRP). Pada penelitian ini, sutera asal Bombyx mori diproduksi menjadi scaffold sutera ukuran pori 100µm kemudian ADSCs dikultur diatas scaffold dalam medium mengandung 10% FBS, 10% FBS-LAA atau 10% PRP. Uji yang dilakukan adalah uji pertumbuhan ADSCs yang dikultur pada polystyrene kemudian uji biokompabilitas scaffold sutera pada ADSCs dalam medium mengandung 10% FBS, 10% FBS-LAA dan 10% PRP. Hasilnya menunjukkan bahwa ketiga kelompok ADSCs dalam variasi medium yang mengandung FBS, FBS-LAA atau PRP dapat mendukung pertumbuhan sel. Ketiga medium tersebut juga tidak berbeda bermakna antar kelompok pada uji biokompabilitas ADSCs yang dikultur pada scaffold sutera. Berdasarkan hasil tersebut, scaffold sutera berpotensi sebagai substrat ADSCs yang dapat dikembangkan sebagai teknologi rekayasa jaringan.

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Published
2020-06-29
How to Cite
ROSADI, Imam et al. Biokompatibilitas scaffold sutera asal Bombyx mori ukuran pori 100µm terhadap adipose-derived stem cells (ADSCs) yang dikultur pada berbagai medium pertumbuhan. Jurnal Biologi Udayana, [S.l.], v. 24, n. 1, p. 7-15, june 2020. ISSN 2599-2856. Available at: <https://ojs.unud.ac.id/index.php/BIO/article/view/61355>. Date accessed: 15 july 2020. doi: https://doi.org/10.24843/JBIOUNUD.2020.v24.i01.p02.