GENE TRANSFORMATION OF ALBUMINE SUNFLOWER (Helianthus annuuss L.) WITH Agrobacterium tumefaciens VECTOR BY IN PLANTA AT SOYBEAN PLANTS
Abstrak
Usage of A. tumefaciens for the purpose of including DNA sunflower albumine into soybean cell, constituted
of natural ability of A. tumefaciens to trasformation in specific form of DNA plasmid (T-DNA) into soybean cell, then integrate at mains soybean cell genom. Result of gene cloning of albumine grown at bacterium of E. coli, then carried over by A. tumefaciens LBA4404, passing method of triparental mating. A.tumefaciens LBA4404 (pAL4404, pSW600) yielded later, then transformation at soybean by in planta. Result of analysis of transformation at soybean of transgenic by in planta, can be proved with PCR analysis.