DESAIN TAQMAN PROBE SECARA IN SILICO SEBAGAI PENDETEKSI MUTASI PADA KODON 516 GEN rpoB Mycobacterium tuberculosis UNTUK METODE REAL-TIME PCR

  • Dek Pueteri Dewi Suryani
  • Putu Sanna Yustiantara
  • Sagung Chandra Yowani
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Abstrak

The aim of this research was to in silico design of TaqMan probe. Design of TaqMan probe were conducted using software Clone Manager Suite 6. As a template, the rpoB gene of M. tuberculosis H37Rv (accession number U12205.1) was used. The results of this research were 8 sequences such as, R516MV-2, R516MV-3, R516MV-4, R516MV-5, R516MV-7, R516MV-8, R516MV-11, R516MV-13. These sequences were met the criteria of TaqMan probe, such as length of nucleotide (23-28 nucleotide), Tm value (72ºC), %GC (50-58%), runs and repeats (?4 bases), dimer structure in accordance to the requirements and does not form hairpin structures. In addition, these sequences were met labeling criteria of TaqMan probe which are including the location of G bases and the number of G-C bases in sequences. Therefore, these sequences could be labeled by FAM (reporter) at 5' end and TAMRA (quencher) at 3' end. The conclusion of this research, the sequences were met the criteria of TaqMan probe. Therefore, it could be targeted to detect mutations at codon 516 with a change of aspartic acid into valine (GAC ? GTC) by using real-time PCR method.

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Dek Pueteri Dewi Suryani

1Jurusan Farmasi FMIPA, Universitas Udayana, Bukit Jimbaran, Bali-Indonesia, 80361

Putu Sanna Yustiantara

1Jurusan Farmasi FMIPA, 2Kelompok Studi MDR-TB & XDR-TB FMIPA, Universitas Udayana, Bukit Jimbaran, Bali-Indonesia, 80361

Sagung Chandra Yowani
1Jurusan Farmasi FMIPA, 2Kelompok Studi MDR-TB & XDR-TB FMIPA, Universitas Udayana, Bukit Jimbaran, Bali-Indonesia, 80361
Diterbitkan
2017-03-31
##submission.howToCite##
SURYANI, Dek Pueteri Dewi; YUSTIANTARA, Putu Sanna; YOWANI, Sagung Chandra. DESAIN TAQMAN PROBE SECARA IN SILICO SEBAGAI PENDETEKSI MUTASI PADA KODON 516 GEN rpoB Mycobacterium tuberculosis UNTUK METODE REAL-TIME PCR. Metamorfosa: Journal of Biological Sciences, [S.l.], v. 4, n. 1, p. 22-28, mar. 2017. ISSN 2655-8122. Tersedia pada: <http://ojs.unud.ac.id/index.php/metamorfosa/article/view/29743>. Tanggal Akses: 12 sep. 2025 doi: https://doi.org/10.24843/metamorfosa.2017.v04.i01.p04.
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Terbitan
Vol 4 No 1 (2017)
Bagian
English

Kata Kunci

rpoB gen, In Silico, TaqMan Probe, Real-time PCR
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License
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