Keragaman Genetik Sekuen Gen ATP Synthase FO Subunit 6 (ATP6) Monyet Hantu (Tarsius) Indonesia (GENETIC DIVERSITY STUDY OF ATP6 GENE SEQUENCES OF TARSIERS FROM INDONESIA)

  • Rini Widayanti Bagian Klinik Hewan, Fakultas Kedokteran Hewan, Universitas Udayana, Bali
  • Niken Satuti Nur Handayani
  • Hery Wijayanto

Abstract

In a conservation effort, the identification of Tarsier species, on the bases of the morphological andmolecular characteristic is necessary. Up to now, the identification of the animals were based on themorphology and vocalizations, which is extremely difficult to identify each, tarsier species. The objective ofthis research was to study the genetic diversity on ATP6 gene of Tarsius sp. Based on sequencing of PCRproduct using primer ATP6F and ATP6R with 681 nts. PCR product. The sequence of ATP6 fragmentswere aligned with other primates from Gene bank with aid of software Clustal W, and were analyzed usingMEGA program version 4.0. Three different nucleotide sites were found (nucleotide no. 288, 321 and 367).The genetic distance based on nucleotide ATP6 sequence calculated using Kimura 2-parameter modelindicated that the smallest genetic distance 0%, biggest 0.8% and average 0, 2%. The phylogenetic treeusing neighbor joining method based on the sequence of nucleotide ATP6 gene could not be used todifferentiate among T. Dianae (from Central Sulawesi), T. Spectrum (from North Sulawesi), T. bancanus(from lampung, South Sumatera) and T.bancanus from West Kalimantan.

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Published
2013-07-21
How to Cite
WIDAYANTI, Rini; HANDAYANI, Niken Satuti Nur; WIJAYANTO, Hery. Keragaman Genetik Sekuen Gen ATP Synthase FO Subunit 6 (ATP6) Monyet Hantu (Tarsius) Indonesia (GENETIC DIVERSITY STUDY OF ATP6 GENE SEQUENCES OF TARSIERS FROM INDONESIA). Jurnal Veteriner, [S.l.], v. 13, n. 4, p. 358-370, july 2013. ISSN 2477-5665. Available at: <http://ojs.unud.ac.id/index.php/jvet/article/view/6027>. Date accessed: 27 sep. 2025.
Citation Formats
Issue
Vol 13 No 4 (2012)
Section
Articles

Keywords

Tarsier sp., ATP6 gene, nucleotide, mt-DNA sequences
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This work is licensed under a Creative Commons Attribution 4.0 International License
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