resep kue basah

cara menghilangkan jerawatdownload clash of clans for pccara alami menghilangkan ketombeCara Alami Menghilangkan Bekas Luka Dengan Cepatberbagai cara menghilangkan bau badancara menghilangkan bau mulut secara alamiJaminan BPKB MobilGadai BPKB Motor Gadai BPKB Mobil Gadai BPKB Truk Pinjam Dana Pinjam UangDana Cepat Jaminan BPKB Mobil Untuk JakartaDana Cepat Jaminan BPKB Mobil Untuk TangerangDana Cepat Jaminan BPKB Mobil Untuk Bekasi

FRAKSINASI AMONIUM SULFAT PADA ENZIM ?-L-ARABINOFURANOSIDASE TERMOSTABIL

I Nengah Wirajana, Ni Nyoman Tri Puspaningsih

Abstract


?-L-Arabinofuranosidases (EC 3.2.1.55) catalyze the hydrolysis ?-L-arabinofuranosidic bonds and act synergistically with other hemicellulases enzymes for the complete hydrolysis of hemicelluloses. Yeast Saccharomyces cerevisiae that considered as Generally Recognized As Safe (GRAS) was chosen for expression and secretion of thermostable ?-L-arabinofuranosidase (AbfA) from Geobacillus thermoleovorans IT-08 termofilik dengan teknologi DNA rekombinan. The extracellular enzyme from the secretion result in this recombinant yeast was precipitated with ammonium sulphate fractionation. Base on the result of measurement of the enzyme activity and the concentration of total protein showed that the specific activity of AbfA enzyme was the lowest at 40% saturation of ammonium sulfat, and the highest at 80% saturation of ammonium sulfat.

Keywords


?-L-arabinofuranosidase, Saccharomyces cerevisiae, Geobacillus thermoleovorans IT-08.

Full Text: PDF

Refbacks

  • There are currently no refbacks.
karimun jawa karimunjawa paket wisata karimunjawa paket karimun jawa