FRAKSINASI DAN IDENTIFIKASI SENYAWA TANIN PADA DAUN BELIMBING WULUH (Averrhoa bilimbi L.)

Abstract

Fractionation of tannic compound from star fruit leaves was conducted by maseration using acetone : water
(7:3), and analytical and preparative thin layer chromatography using a variety of eluents. The isolates obtained were
identified using UV-Vis and FT-IR Spectrophotometer.
The best eluent for both analytical and preparative TLC was found to be n-butanol : water : acetic acid
(4:1:5), which gave three isolates with Rf of 0.53, 0.61, and 0.68. The second isolate (Rf = 0.61), showed a maximum
absorption at 331 nm on the UV-Vis spectra. Its IR spectra showed specific absorptions such as OH-assymetric
stretch (3372.2 cm-1), aromatic overtone (2071.8 cm-1), and aromatic and benzene stretches at 1625.8 cm-1 and 782.5
cm-1 respectively. These spectra suggest that the tannic compound was either flavan pent-3,6,7,4',5'-ol or flavan
pent-3,7,8,4',5'-ol.