THE EFFECT OF ADMINISTRATION OF ETHANOL EXTRACT OF RED GALANGA (Alpinia purpurata K. Schum) RHIZOMES ON THE HISTOPATHOLOGY OF WISTAR RATS BREAST CANCER CELLS INDUCED BY DMBA (7,12-dimetilbenz[a]antrasena)
Abstract
Rimpang lengkuas merah (Alpinia purpurata K.Schum) merupakan tanaman yang berpotensi sebagai produk alami. Produk alami ini memiliki aktivitas yang bergantung pada jumlah senyawa aktif yang diekstraksi dan metode ekstraksi. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian suplementasi etanol rimpang lengkuas merah terhadap perubahan histologis kanker payudara. Metode yang digunakan adalah metode skrining fitokimia, identifikasi menggunakan instrumen LC-MS/MS, pemeriksaan histopatologi jaringan kelenjar payudara, DMBA, rotary evaporator vakum, dan seperangkat alat LCMS/MS ACQUITY UPLC®H-Class System (Water, AS). Rimpang lengkuas merah (Alpinia purpurata K.Schum) diperoleh dari Desa Badung dan diidentifikasi di Pusat Konservasi Tumbuhan LIPI-UPT Kebun Raya “Eka Karya” Bali. Rimpang lengkuas merah dengan induksi DMBA pada berbagai dosis menunjukkan bahwa ekstrak etanol rimpang lengkuas merah berpengaruh dalam memperbaiki gambaran histopatologi sel kanker payudara mencit pada dosis 70 mg/kgBB/hari dan 90 mg/kgBB/hari.
Kata kunci: Antikanker, kanker payudara, DMBA, histopatologi, rimpang lengkuas merah
ABSTRACT
The red galangal rhizomes (Alpinia purpurata K.Schum) plant has natural product potential. The Natural product has the activity of a plant, which depends on the amount of active compound extracted and the extraction method. This study aimed to determine the effect of the administration of ethanol on supplementing red galanga rhizomes on histological changes in breast cancer. Using phytochemical screening methods, identification using LC-MS/MS instruments, histopathological examination of breast glandular tissue, DMBA, a Vacum rotary evaporator, a set of tools, and an LC-MS/MS ACQUITY UPLC®H-Class System (Waters, USA). The red galangal rhizome (Alpinia purpurata K.Schum) was obtained from Badung village and determined at the LIPI-UPT Center for Plant Conservation of the Bali “Eka Karya” Botanical Garden. The red galanga rhizomes reported that the higher the ethanol concentration, the higher the perhexiline content was thought to be anti-breast cancer. Phytosphingosine had the anticancer activity of red galanga rhizomes, with DMBA induction at various doses showing that the ethanol extract of red galangal rhizomes had an effect in improving the histopathological picture of mouse breast cancer cells at doses of 70 mg/kgBW/day and 90 mg/kgBW/day.
Keywords: Anticancer, breast cancer, DMBA, histopathology, red galanga rhizomes
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References
Agustina, S., Ruslan, and Wiraningtyas, A. 2016. Skrining Fitokimia Tanaman Obat di Kabupaten Bima. Cakra Kimia. 4(1): 71-75
Chlipala, E., Bendzinski, C. M., Chu, K., Johnson, J. I., Brous, M., Copeland, K., & Bolon, B. 2020. Optical Density-Based Image Analysis Method for the Evaluation of Hematoxylin and Eosin Staining Precision. Journal of histotechnology. 43(1): 29-37
Dhakal, B., Tomita, Y., Drew, P., Price, T., Maddern, G., Smith, E., & Fenix, K. 2023. Perhexiline: Old Drug, New Tricks? A Summary of Its Anti-Cancer Effects. Molecules. 28(8): 3624
Digambiro, R. A., and Parwanto, E. 2024. Panduan Prosesing dan Pewarnaan Jaringan dalam Histopatologi. Penerbit Lakeisha. Klaten
Halim, R. 2018. Asam Cuka Sebagai Agen Deparafinisasi pada Pengecatan Hematoksilin Eosin (HE). Doctoral dissertation. Universitas Muhammadiyah Semarang
Huang, J., Chan, PS, Lok, V., Chen, X., Ding, H., Jin, Y., & Wong, MC 2021. Insiden Global dan Kematian Akibat Kanker Payudara: Analisis Tren. Penuaan (Albany NY). 13(4): 5748
Kementerian Kesehatan Republik Indonesia. 2018. Hasil Utama RISKESDAS
Ledet, M. M., Oswald, M., Anderson, R., & Van de Walle, G. R. 2018. Differential signaling pathway activation in 7, 12-dimethylbenz [a] anthracene (DMBA)-treated mammary stem/progenitor cells from species with varying mammary cancer incidence. Oncotarget. 9(67): 32761
Liangan, R., Kairupan, C., & Durry, M. 2015. Pengaruh Pemberian Ekstrak Lengkuas (Alpinia galanga) Terhadap Gambaran Histologik Payudara Mencit (Mus musculus) yang Diinduksi Benzo(a)pyrene. eBiomedik. 3(1): 480-484
Liu, D., You, P., Luo, Y., Yang, M., & Liu, Y. 2018. Galangin Induces Apoptosis In MCF-7 Human Breast Cancer Cells Through Mitochondrial Pathway and Phosphatidylinositol 3-Kinase/Akt Inhibition. Pharmacology. 102(1-2): 58-66
Mamay, Mutmaina, G. N., dan Nurahma, I. A. 2022. Penggunaan Sabun Pencuci Piring Sebagai Pengganti Xilol Dalam Proses Deparafinasi Pewarnaan Hematoksilin-Eosin. Medicra (Journal of Medical Laboratory Science Technology). 5(1): 47 – 55
Mangurana, W. O. I., Yusnaini, Y., & Sahidin, S. 2019. Analisis LC-MS/MS (Liquid Crhomatogaph Mass Spectrometry) dan Metabolit Sekunder Serta Potensi Antibakteri Ekstrak n-heksana Spons Callyspongia aerizusa yang Diambil pada Kondisi Tutupan Terumbu Karang Yang Berbeda di Perairan Teluk Staring. Jurnal biologi tropis. 19(2): 131-141
Pangestuti, D., Harris, B., & Rahman, T. K. 2020. Comparison of the Effectiveness between Red Galangal Rhizome's (Alpinia purpurata K. schum) Extract and Ketoconazol 2% to Inhibit Growth of Dermatophyte Fungi In Vitro. Buletin Farmatera. 5(1): 156-162
Pramushinta, I. A. K., & Ajiningrum, P. S. 2017. Uji Aktivitas Sel Kanker dengan menggunakan senyawa Flavonoid dari Lengkuas (Alpinia Galanga). STIGMA: Jurnal Matematika dan Ilmu Pengetahuan Alam Unipa. 10(2): 89 – 91
Putri, D. M., and Lubis, S. S. 2020. Skrining Fitokimia Ekstrak Etil Asetat Daun Kalayu (Erioglossum rubiginosum (Roxb.) Blum). Amina. 2(3): 120-125
Ravindran, R., Sruthi, A. K., Ameena, M., & Harish, R. N. K. D. 2018. Bleached Palm Oil as a Bio-friendly Substitute for Xylene: A Comparative Study. Oral & Maxillofacial Pathology Journal. 9(2): 63-69
Sharifi-Rad, J., Seidel, V., Izabela, M., Monserrat-Mequida, M., Sureda, A., Ormazabal, V., & Cho, W. C. 2023. Phenolic compounds as Nrf2 inhibitors: potential applications in cancer therapy. Cell Communication and Signaling. 21(1): 89
Soesilawati, P. 2020. Histologi Kedokteran Dasar. Airlangga University Press. Surabaya
Wijaya, A., and Noviana, N. 2022. Penetapan Kadar Air Simplisia Daun Kemangi (Ocimum basilicum L.) Berdasarkan Perbedaan Metode Pengeringan. Jurnal Riset Kefarmasian Indonesia. 4(2): 185-194
Wuyung, P. E. 2016. Induksi DMBA dalam Karsinogenesis Kelenjar Payudara. Pratista Patologi. 5(1): 44-51
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