THE PRODUCTION OF ANTI-Dirofilaria immitis ANTIBODIES FOR THE DIAGNOSIS DEVELOPMENT OF DIROFILARIASIS IN DOGS
Abstract
Dirofilaria immitis (D. immitis) as a causative agent of heartworm disease is not only caused problems in animals but also zoonoses. For diagnosis of dirofilariasis (disease caused by D. immitis) serologically is needed anti-D. immitis antibodies. The objective of this study was to produce antibodies against excretory-secretory antigens produced by male and female worms for developing the diagnosis of dirofilariasis based on the antigens detection. Base on this study can be concluded that male excretory-secretory antigens (MES), female excretory-secretory antigens (FES), and MES+FES can stimulate BALB/c mouse to produce polyclonal antibodies in the same pattern. Antibodies have been produced at day 21 and the peak titter was at day 35 after first immunization.
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References
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Aranda C, Panyella O, Eritja R, Castella J. 1998. Canine filariasis importance and transmission in the Baix Llobregat area, Barcelona (Spain). Vet Parasitol, 77: 267–275.
Bolio-Gonzalez ME, Rodriguez-Vivas RI, Sauri-Arceo CH, Gutierrez-Blanco E, Ortega-Pacheco A, Colin-Flores RF. 2007. Prevalence of the Dirofilaria immitis infection in dogs from Merida, Yucatan, Mexico. Vet Parasitol, 148: 166–169.
Cruz-Chan JV, Quijano-Hernandez I, Ramirez-Sierra MJ, Dumonteil E. 2009. Dirofilaria immitis and Trypanosoma cruzi natural co-infection in dogs. The Vet J doi: 10.1016/j.tvjl.2009. 09.012.
Genchi C, Rinaldi L, Mortarino M, Genchi M, Cringoli G. 2009. Climate and Dirofilaria infection in Europe. Vet Parasitol, 163: 286–292.
Goodwin J-K. 1998. The serologis diagnosis of heartworm infection in dogs and cats. Clinical Techniques in Small Animals Practice, 13: 83–87.
Grieve RB, Mika-Johnson M, Jacobson RH, Cypress RH. 1981. Enzyme-linked immunosorbent assay for measurement of antibody responses to Dirofilaria immitis in experimentally infected dogs. Am J Vet Res, 42: 66–69.
Hayasaki M, Nakagaki K, Kobayashi S, Ahishi I. 1981. Immunological response of dogs to Dirofilaria immitis infection. Jpn J Vet Sci, 43: 909–914.
Maizels RM, Blaxter ML, Robertson BD, Selkirk ME. 1991. Parasite Antigens, Parasite Genes. A Laboratory Manual for Molecular Parasitology. Cambridge University Press, New York, Port Chester, Melbourne, Sydney.
Marcos-Atzutegi C, Kramer LH, Fernandez I, Simoncini L, Genchi M, Prieto G, Simon F. 2003. TH1 response in BALB/c mice immunized with Dirofilaria immitis soluble antigens: a possible role for Wolbachia? Vet Parasitol, 112: 117–130.
Meriem-Hind BM, Mohamed M. 2009. Prevalence of canine Dirofilaria immitis infection in the city of Algiers, Algeria. African J of Agri Res, 4: 1097–1100.
Reifur L, Thomaz-Socco V, Montiani-Ferreira F. 2004. Epidemiological aspects of filariosis in dogs on the coast of Parana state, Brazil: with emphasis on Dirofilaria immitis. Vet Parasitol, 122: 273–286.
Song KH, Hayasaki M, Cholic C, Cho KW, Han HR, Jeong BH, Jeon MH, Park BK, Kom DH. 2002. Immunological responses of dogs experimentally infected with Dirofilaria immitis. J Vet Sci, 3: 109–114.
Suyoko. 1998. Pengembangan antibodi monoklonal spesifik terhadap antigen beredar Brugia malayi untuk diagnosis filariasis malayi. Disertasi. Universitas Gadjah Mada.
Weil GJ, Ottesen EA. 1981. Dirofilaria immitis: parasiti-specific humoral and cellular immune responses in experimentally infected dogs. Exp Parasitol, 51: 80–86.
Weil GJ. 1987. Dirofilaria immitis: Identification and Partial Characterization of Parasite Antigens in the Serum of Infected Dogs. Exp Parasitol, 64: 244–251.
Published
2016-08-02
How to Cite
ERAWAN, I Gusti Made Krisna et al.
THE PRODUCTION OF ANTI-Dirofilaria immitis ANTIBODIES FOR THE DIAGNOSIS DEVELOPMENT OF DIROFILARIASIS IN DOGS.
Buletin Veteriner Udayana, [S.l.], aug. 2016.
ISSN 2477-2712.
Available at: <https://ojs.unud.ac.id/index.php/buletinvet/article/view/23306>. Date accessed: 23 apr. 2024.
Section
Articles
Keywords
Dirofilaria immitis; antibodi; antigen ekskretori-sekretori; antibodies; excretory-secretory antigens
