THE ROLE OF RECOMBINANT IL-10 ON THE SERUM LEVEL OF TNF-?, ONE HOUR POST TRAUMATIC BRAIN INJURY OF THE WISTAR RAT

  • Willy Adhimarta School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
  • Andi Asadul Islam School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
  • Sri Maliawan School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
  • Gatot S. Lowrence School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
  • Ilhamjaya Patellongi School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia

Abstract

Background: Brain injury often occurs not only primary brain injury, but often also occur secondary brain injury. Inflammation is a process that occurs immediately after trauma characterized by activation of the mediator substance. TNF-? is a major cytokinee involved in the inflammatory processes that have adverse effects if the serum level are excessive. There needs to be a balance of the inflammatory process in the brain injury so things that harm does not occur. As anti-inflammatory IL- 10 plays an important role in maintaining the balance. The objective of this study is to determine the effect of IL-10 intervention as an anti -inflammatory will decrease the serum level of TNF-? in traumatic brain injury. Material And Method: Experimental Study in the Rattus Wistar rats, post test control group design, male, aged 3-4 months, with body weight (BW) 300-400g, were obtained from the Laboratory Animal Faculty of Medicine, University of Hasanuddin as much as 24 tails, which is the result of breeding. Subjects were divided into four groups, each group of six rats, treated with controlled cortical impact model (Feeney’s weight-drop) of traumatic brain injury. Blood taken with capillary tube in retro-orbita plexus or sinus.This study has approved by ethical clearance for research. Results: Levels of TNF-? group of rats 1 hour post-trauma without administration of recombinant IL-10 (28.58 ± 7.28) pg / mL; was significantly higher (p <0.05) than the levels of TNF- ? rats without kraniektomi group (22.06 ± 3.34) pg / mL and group kraniektomi rats without brain injury (23.07 ± 2.51) pg / mL. Levels of TNF-? group of rats 1 hour post-trauma by administration of recombinant IL-10 (23.39 ± 6.30) pg / mL; significantly lower (p <0.05) than the group of rats 1 hour post-trauma without administration of recombinant IL-10; and did not different significantly (p> 0.05) in the group without craniectomy or craniectomy group without head injury. Conclusions: Intervention of recombinant IL-10 decreases levels of TNF-? serum soon after traumatic brain injury in rats.

Downloads

Download data is not yet available.

Author Biographies

Willy Adhimarta, School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
Andi Asadul Islam, School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
Sri Maliawan, School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
Gatot S. Lowrence, School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
Ilhamjaya Patellongi, School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
School of Medicine Surgery Sub Neurosurgery, Hasanuddin University, Makassar, South Sulawesi, Indonesia
Published
2015-04-19
How to Cite
ADHIMARTA, Willy et al. THE ROLE OF RECOMBINANT IL-10 ON THE SERUM LEVEL OF TNF-?, ONE HOUR POST TRAUMATIC BRAIN INJURY OF THE WISTAR RAT. BALI MEDICAL JOURNAL, [S.l.], v. 4, n. 1, apr. 2015. ISSN 2302-2914. Available at: <http://ojs.unud.ac.id/index.php/bmj/article/view/21691>. Date accessed: 21 aug. 2025.
Citation Formats
Issue
Vol 4 No 1 (2015)
Section
Articles

Keywords

TNF-?; IL-10; traumatic; brain; injury.
Warning: array_merge(): Argument #2 is not an array in /var/www/ojs.unud.ac.id_backup/lib/pkp/classes/core/PKPApplication.inc.php on line 578 Warning: Invalid argument supplied for foreach() in /var/www/ojs.unud.ac.id_backup/plugins/generic/recommendByAuthor/RecommendByAuthorPlugin.inc.php on line 114