PENGARUH TOTAL FENOLTERIPANG PASIR (Holothuria scabra) TERHADAP RESPON IMUN NON SPESIFIK IKAN MAS (Cyprinus carpio)

  • Achmad Suhermanto

Abstract

Abstract
The aim of the study was to know administration and applications of optimal doses of sea
cucumber total phenol to increase nonspecific immune response of Carp. Bioactive component
extraction was done by methanol and for further fractionation was dissolved with ethyl
acetate (v/v). Total phenol identification on ethyl acetate fraction was done to utilize UVVis
spectrophotometer and infrared. Total phenol produced was tested on carp with
intraperitonial injection, the concentration of 0; 0,09; 0,18; and 0,27 mg phenols/kg fish..
Challenge be done utilizes A. hydrophila (107cell/ml) with immertion method. Statistical
analysis involved one-way analysis of varians (ANOVA) by minitab 14. The level of
significance were expressed as P-value less or greater than 0.05.
The result of hematological parameters showed that hematocrit (PVC), hemoglobin,
erythrocytes, lymphocyte pre infection was increased significantly and post-bacterial was
decreased significantly (p<0.05). Leukocytes, Neutrophils pre-and post-infection were
significantly increased (p<0.05). Eosinophils, monocytes pre-and post bacterial infection
were not differ significantly between treatment (p<0.05).

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Author Biography

Achmad Suhermanto
Akademi Perikanan Sorong (APSOR), KKD-BP Kesehatan Ikan dan Lingkungan, Jl. K. Pattimura,
Tanjung Kasuari,PO Box 109, Sorong 98410, Papua Barat, Indonesia.
Published
2013-08-01
How to Cite
SUHERMANTO, Achmad. PENGARUH TOTAL FENOLTERIPANG PASIR (Holothuria scabra) TERHADAP RESPON IMUN NON SPESIFIK IKAN MAS (Cyprinus carpio). Bumi Lestari, [S.l.], v. 13, n. 2, aug. 2013. ISSN 2527-6158. Available at: <https://ojs.unud.ac.id/index.php/blje/article/view/6639>. Date accessed: 20 apr. 2024.
Citation Formats
Issue
Vol 13 No 2 (2013)
Section
Original Research Articles

Keywords

Key Words :Holothuria scabra, Cyprinus carpio, Aeromonas hydrophila, nonspecific immune response

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